Understanding the Dynamic Behavior of an Anticancer Drug, Doxorubicin, on a Lipid Membrane Using Multiple Spectroscopic Techniques

2019 ◽  
Vol 123 (17) ◽  
pp. 3756-3762 ◽  
Author(s):  
Yi Hou ◽  
Shun-Li Chen ◽  
Wei Gan ◽  
Xing Ma ◽  
Qunhui Yuan
Keyword(s):  
Dynamic Behavior ◽  
Anticancer Drug ◽  
Lipid Membrane ◽  
Spectroscopic Techniques

scholarly journals Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Partha Sarathi Guin ◽  
Saurabh Das
Keyword(s):  
Cyclic Voltammetry ◽  
Anticancer Drug ◽  
Dna Interaction ◽  
Calf Thymus Dna ◽  
Potential Range ◽  
Spectroscopic Techniques ◽  
Doxorubicin Hydrochloride ◽  
Cathodic Peak ◽  
Peak Current ◽  
Calf Thymus

Electrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction withE1/2value −665±5 mV (versus Ag/AgCl, saturated KCl). Depending on scan rates, processes were either quasireversible (at low scan rates) or near perfect reversible (at high scan rates). This difference in behavior of doxorubicin hydrochloride with scan rate studied over the same potential range speaks of differences in electron transfer processes in doxorubicin hydrochloride. Attempt was made to identify and understand the species involved using simulation. The information obtained was used to study the interaction of doxorubicin hydrochloride with calf thymus DNA. Cathodic peak current gradually decreased as more calf thymus DNA was added. The decrease in cathodic peak current was used to estimate the interaction of the drug with calf thymus DNA. Nonlinear curve fit analysis was applied to evaluate the intrinsic binding constant and site size of interaction that was compared with previous results on doxorubicin hydrochloride-DNA interaction monitored by cyclic voltammetry or spectroscopic techniques.

Investigation of anticancer drug lapatinib and its interaction with dsDNA by electrochemical and spectroscopic techniques

2014 ◽  
Vol 194 ◽  
pp. 185-194 ◽  
Author(s):  
Burcu Dogan-Topal ◽  
Burcin Bozal-Palabiyik ◽  
Sibel A. Ozkan ◽  
Bengi Uslu
Keyword(s):  
Anticancer Drug ◽  
Spectroscopic Techniques

Dynamic behavior analysis of ion transport through a bilayer lipid membrane by an electrochemical method combined with fluorometry

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3839-3845
Author(s):  
Terumasa Omatsu ◽  
Kisho Hori ◽  
Yasuhiro Naka ◽  
Megumi Shimazaki ◽  
Kazushige Sakai ◽  
...  
Keyword(s):  
Ion Transport ◽  
Behavior Analysis ◽  
Dynamic Behavior ◽  
Electrochemical Method ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Membrane Conductivity ◽  
Bilayer Lipid

The ion transport through a bilayer lipid membrane was analyzed by an electrochemical method combined with fluorometry. The distribution of a cation and an anion predominantly determines membrane conductivity.

Excited state proton transfer dynamics of an eminent anticancer drug, ellipticine, in octyl glucoside micelle

2014 ◽  
Vol 16 (28) ◽  
pp. 14953-14960 ◽  
Author(s):  
Krishna Gavvala ◽  
Raj Kumar Koninti ◽  
Abhigyan Sengupta ◽  
Partha Hazra
Keyword(s):  
Excited State ◽  
Steady State ◽  
Proton Transfer ◽  
Anticancer Drug ◽  
Photophysical Properties ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Octyl Glucoside ◽  
Excited State Proton Transfer

Prototropical and photophysical properties of an anticancer drug, ellipticine, are explored inside the octyl-β-d-glucoside micelles using steady state and time-resolved fluorescence spectroscopic techniques.

Differential polarization imaging of sickled cells

1988 ◽  
Vol 46 ◽  
pp. 62-63
Author(s):  
Marcos F. Maestre
Keyword(s):  
Optical Properties ◽  
Theoretical Approach ◽  
Polarized Light ◽  
Polarization Microscopy ◽  
Spectroscopic Techniques ◽  
Polarization Imaging ◽  
Circularly Polarized Light ◽  
Circularly Polarized ◽  
Microscopic Scale ◽  
Chiral Structures

Recently we have developed a form of polarization microscopy that forms images using optical properties that have previously been limited to macroscopic samples. This has given us a new window into the distribution of structure on a microscopic scale. We have coined the name differential polarization microscopy to identify the images obtained that are due to certain polarization dependent effects. Differential polarization microscopy has its origins in various spectroscopic techniques that have been used to study longer range structures in solution as well as solids. The differential scattering of circularly polarized light has been shown to be dependent on the long range chiral order, both theoretically and experimentally. The same theoretical approach was used to show that images due to differential scattering of circularly polarized light will give images dependent on chiral structures. With large helices (greater than the wavelength of light) the pitch and radius of the helix could be measured directly from these images.

Characterization of intergranular cuprous oxide in polycrystalline YBa2Cu3O7-x

1988 ◽  
Vol 46 ◽  
pp. 880-881
Author(s):  
Bradley L. Thiel ◽  
Chan Han R. P. ◽  
Kurosky L. C. Hutter ◽  
I. A. Aksay ◽  
Mehmet Sarikaya
Keyword(s):  
Grain Boundary ◽  
Cuprous Oxide ◽  
Room Temperature ◽  
Boundary Phase ◽  
Spectroscopic Techniques ◽  
Transition Width ◽  
Practical Applications ◽  
Thin Foils ◽  
Superconducting Oxides

The identification of extraneous phases is important in understanding of high Tc superconducting oxides. The spectroscopic techniques commonly used in determining the origin of superconductivity (such as RAMAN, XPS, AES, and EXAFS) are surface-sensitive. Hence a grain boundary phase several nanometers thick could produce irrelevant spectroscopic results and cause erroneous conclusions. The intergranular phases present a major technological consideration for practical applications. In this communication we report the identification of a Cu2O grain boundary phase which forms during the sintering of YBa2Cu3O7-x (1:2:3 compound).Samples are prepared using a mixture of Y2O3. CuO, and BaO2 powders dispersed in ethanol for complete mixing. The pellets pressed at 20,000 psi are heated to 950°C at a rate of 5°C per min, held for 1 hr, and cooled at 1°C per min to room temperature. The samples show a Tc of 91K with a transition width of 2K. In order to prevent damage, a low temperature stage is used in milling to prepare thin foils which are then observed, using a liquid nitrogen holder, in a Philips 430T at 300 kV.

Sign in / Sign up

Export Citation Format

Share Document