Three-State DNA Hairpin Conformational Dynamics Revealed by Higher-Order Fluorescence Correlation Spectroscopy

2019 ◽  
Vol 123 (7) ◽  
pp. 1491-1504 ◽  
Author(s):  
Farshad Abdollah-Nia ◽  
Martin P. Gelfand ◽  
Alan Van Orden
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Conformational Dynamics ◽  
Higher Order ◽  
Correlation Spectroscopy ◽  
Dna Hairpin ◽  
Fluorescence Correlation

Conformational dynamics of Calcium Green 1 by fluorescence correlation spectroscopy

1999 ◽  
Vol 1 (24) ◽  
pp. 5767-5769 ◽  
Author(s):  
Adeline Tessier ◽  
Rachel Me´allet-Renault ◽  
Patrick Denjean ◽  
Dominique Miller ◽  
Robert B. Pansu
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Calcium Green

scholarly journals Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2972 ◽  
Author(s):  
Takuhiro Otosu ◽  
Shoichi Yamaguchi
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Single Molecule Level ◽  
Fluorescence Correlation ◽  
Promising Tool ◽  
Molecular Ruler

We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime is sensitive to the microenvironment and can be a “molecular ruler” when combined with FRET. Utilization of fluorescence lifetime in 2D FLCS thus enables us to quantify the inhomogeneity of the system and the interconversion dynamics among different species with a higher time resolution than other single-molecule techniques. Recent applications of 2D FLCS to various biological systems demonstrate that 2D FLCS is a unique and promising tool to quantitatively analyze the microsecond conformational dynamics of macromolecules at the single-molecule level.

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