scholarly journals Advances in the Structural Design of Polyelectrolyte Complex Micelles

2021 ◽  
Author(s):  
Alexander E. Marras ◽  
Jeffrey M. Ting ◽  
Kaden C. Stevens ◽  
Matthew V. Tirrell
Keyword(s):  
Structural Design ◽  
Polyelectrolyte Complex ◽  
Complex Micelles

scholarly journals Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2553
Author(s):  
Jeffrey M. Ting ◽  
Alexander E. Marras ◽  
Joseph D. Mitchell ◽  
Trinity R. Campagna ◽  
Matthew V. Tirrell
Keyword(s):  
Ethylene Glycol ◽  
Polyelectrolyte Complex ◽  
Serum Proteins ◽  
Raft Polymerization ◽  
Sodium Acrylate ◽  
Structure Property ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Complex Micelles

A series of model polyelectrolyte complex micelles (PCMs) was prepared to investigate the consequences of neutral and zwitterionic chemistries and distinct charged cores on the size and stability of nanocarriers. Using aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization, we synthesized a well-defined diblock polyelectrolyte system, poly(2-methacryloyloxyethyl phosphorylcholine methacrylate)-block-poly((vinylbenzyl) trimethylammonium) (PMPC-PVBTMA), at various neutral and charged block lengths to compare directly against PCM structure–property relationships centered on poly(ethylene glycol)-block-poly((vinylbenzyl) trimethylammonium) (PEG-PVBTMA) and poly(ethylene glycol)-block-poly(l-lysine) (PEG-PLK). After complexation with a common polyanion, poly(sodium acrylate), the resulting PCMs were characterized by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). We observed uniform assemblies of spherical micelles with a diameter ~1.5–2× larger when PMPC-PVBTMA was used compared to PEG-PLK and PEG-PVBTMA via SAXS and DLS. In addition, PEG-PLK PCMs proved most resistant to dissolution by both monovalent and divalent salt, followed by PEG-PVBTMA then PMPC-PVBTMA. All micelle systems were serum stable in 100% fetal bovine serum over the course of 8 h by time-resolved DLS, demonstrating minimal interactions with serum proteins and potential as in vivo drug delivery vehicles. This thorough study of the synthesis, assembly, and characterization of zwitterionic polymers in PCMs advances the design space for charge-driven micelle assemblies.

scholarly journals Structure–Property Relationships of Oligonucleotide Polyelectrolyte Complex Micelles

Nano Letters ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 7111-7117 ◽  
Author(s):  
Michael Lueckheide ◽  
Jeffrey R. Vieregg ◽  
Alex J. Bologna ◽  
Lorraine Leon ◽  
Matthew V. Tirrell
Keyword(s):  
Polyelectrolyte Complex ◽  
Structure Property ◽  
Structure Property Relationships ◽  
Complex Micelles

scholarly journals Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles

2014 ◽  
Vol 2 (46) ◽  
pp. 8142-8153 ◽  
Author(s):  
Cheng-Hsiang Kuo ◽  
Lorraine Leon ◽  
Eun Ji Chung ◽  
Ru-Ting Huang ◽  
Timothy J. Sontag ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nucleic Acids ◽  
Enzymatic Degradation ◽  
Polyelectrolyte Complex ◽  
Specific Interactions ◽  
Delivery Vehicles ◽  
Complex Micelles ◽  
Gene Delivery Vehicles

Polyelectrolyte complex micelles have great potential as gene delivery vehicles because of their ability to encapsulate charged nucleic acids forming a core by neutralizing their charge, while simultaneously protecting the nucleic acids from non-specific interactions and enzymatic degradation.

Effects of Polyelectrolyte Complex Micelles and Their Components on the Enzymatic Activity of Lipase

Langmuir ◽  
2010 ◽  
Vol 26 (12) ◽  
pp. 9802-9808 ◽  
Author(s):  
Saskia Lindhoud ◽  
Willem Norde ◽  
Martien A. Cohen Stuart
Keyword(s):  
Enzymatic Activity ◽  
Polyelectrolyte Complex ◽  
Complex Micelles

Cationic drug-based self-assembled polyelectrolyte complex micelles: Physicochemical, pharmacokinetic, and anticancer activity analysis

2016 ◽  
Vol 146 ◽  
pp. 152-160 ◽  
Author(s):  
Thiruganesh Ramasamy ◽  
Bijay Kumar Poudel ◽  
Himabindu Ruttala ◽  
Ju Yeon Choi ◽  
Truong Duy Hieu ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Polyelectrolyte Complex ◽  
Activity Analysis ◽  
Self Assembled ◽  
Complex Micelles

scholarly journals Mechanism of Dissociation Kinetics in Polyelectrolyte Complex Micelles

2019 ◽  
Author(s):  
Hao Wu ◽  
Jeffrey Ting ◽  
Matthew Tirrell
Keyword(s):  
Light Scattering ◽  
Polyelectrolyte Complex ◽  
Environmental Changes ◽  
Environmental Parameters ◽  
Underlying Mechanism ◽  
Scattering Data ◽  
Relaxation Rates ◽  
Dissociation Kinetics ◽  
Complex Micelles ◽  
Kinetics Of

<div> <div> <p>Polyelectrolyte-based nanoscale self-assemblies, such as micelles, possess diverse desirable attributes such as capability for sequestering and protecting biomacromolecules against inhospitable environments, responsiveness to external stimuli, and tunability of physical behavior. However, little is known on the mechanisms of dissociation when micelles encounter and respond to environmental changes. Using salt-jump, time-dependent, light scattering, the pathway of dissociation is observed in polyelectrolyte complex micelles that have complex cores and neutral coronas. The micelle dissociation kinetics appear to be a three-staged process, in good agreement with the scattering data. Using kinetic models of amphiphilic block copolymer micelles in polyelectrolyte complexation-driven micelles, we derive an analytical expression for dissociation relaxation rates as a function of solvent temperature, salt concentration, and the length of the charged polymer blocks. The theoretical predictions are compatible with the experimental data from light scattering experiments. This study demonstrates experimentally the relaxation kinetics of polyelectrolyte complex micelle dissociation and illustrates the underlying mechanism governing the dissociation kinetics. It is anticipated that these findings can be generalized to other electrostatic interaction-driven self-assemblies to better understand the relationship among the kinetics of dissociation, constituent polymer properties, and environmental parameters.<br></p> </div> </div>

scholarly journals Assembly and Characterization of Polyelectrolyte Complex Micelles

2019 ◽  
Author(s):  
Alexander Marras ◽  
Jeffrey Vieregg ◽  
Matthew Tirrell
Keyword(s):  
Block Copolymers ◽  
Polyelectrolyte Complex ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Representative Data ◽  
Core Shell Nanoparticles ◽  
Complex Micelles

We provide protocols and representative data for designing, assembling, and characterizing polyelectrolyte complex micelles (core-shell nanoparticles formed by polyelectrolytes and hydrophilic charged-uncharged block copolymers).

scholarly journals Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

2020 ◽  
Author(s):  
Jeffrey Ting ◽  
Alexander Marras ◽  
Joseph Mitchell ◽  
Trinity Campagna ◽  
Matthew Tirrell
Keyword(s):  
Ethylene Glycol ◽  
Polyelectrolyte Complex ◽  
Serum Proteins ◽  
Sodium Acrylate ◽  
Structure Property ◽  
Poly Ethylene Glycol ◽  
Core Diameter ◽  
Poly Ethylene ◽  
Complex Micelles

<p>A series of model polyelectrolyte complex micelles (PCMs) was prepared to investigate the consequences of neutral and zwitterionic chemistries and distinct charged cores on the size and stability of nanocarriers. Using aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization, we synthesized a well-defined diblock polyelectrolyte system, poly(2-methacryloyloxyethyl phosphorylcholine methacrylate)-<i>block</i>-poly((vinylbenzyl) trimethylammonium) (PMPC-PVBTMA), at various neutral and charged block lengths to compare directly against PCM structure-property relationships centered on poly(ethylene glycol)-<i>block</i>-poly((vinylbenzyl) trimethylammonium) (PEG-PVBTMA) and poly(ethylene glycol)-<i>block</i>-poly(lysine) (PEG-PLK). After complexation with a common polyanion, poly(sodium acrylate), the resulting PCMs were characterized by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). We observed uniform assemblies of spherical micelles with a core diameter of ~40 nm when PMPC-PVBTMA was used, and smaller particles between 20-30 nm for PEG-PLK and PEG-PVBTMA via SAXS analysis. Additionally, PEG-PLK PCMs proved most resistant to dissolution by both monovalent and divalent salt, followed by PEG-PVBTMA then PMPC-PVBTMA. All micelle systems were serum stable in 100% fetal bovine serum over the course of 8 h by time-resolved DLS, demonstrating minimal interactions with serum proteins and potential as in vivo drug delivery vehicles. This thorough study of the synthesis, assembly, and characterization of zwitterionic polymers in PCMs advances the design space for charge-driven micelle assemblies.</p>

pH sensitive polyelectrolyte complex micelles for highly effective combination chemotherapy

2014 ◽  
Vol 2 (37) ◽  
pp. 6324 ◽  
Author(s):  
Thiruganesh Ramasamy ◽  
Jeong Hwan Kim ◽  
Ju Yeon Choi ◽  
Tuan Hiep Tran ◽  
Han-Gon Choi ◽  
...  
Keyword(s):  
Combination Chemotherapy ◽  
Polyelectrolyte Complex ◽  
Ph Sensitive ◽  
Effective Combination ◽  
Highly Effective ◽  
Complex Micelles
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