Bioactive Sesquiterpenes from the Edible Mushroom Flammulina velutipes and Their Biosynthetic Pathway Confirmed by Genome Analysis and Chemical Evidence

2016 ◽  
Vol 81 (20) ◽  
pp. 9867-9877 ◽  
Author(s):  
Qiaoqiao Tao ◽  
Ke Ma ◽  
Yanlong Yang ◽  
Kai Wang ◽  
Baosong Chen ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Biosynthetic Pathway ◽  
Edible Mushroom ◽  
Flammulina Velutipes ◽  
Chemical Evidence

scholarly journals Draft Genome Sequence of the Basidiomycetous Fungus Flammulina velutipes TR19

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Atsushi Kurata ◽  
Yasuhisa Fukuta ◽  
Miho Mori ◽  
Noriaki Kishimoto ◽  
Norifumi Shirasaka
Keyword(s):  
Genome Sequence ◽  
Genome Analysis ◽  
Draft Genome ◽  
Flammulina Velutipes ◽  
Draft Genome Sequence ◽  
Whole Genome ◽  
Fruiting Body Formation ◽  
Whole Genome Analysis ◽  
Body Formation ◽  
Basidiomycetous Fungus

Here, we report the draft genome sequence of Flammulina velutipes TR19, which was newly isolated from commercial strains in Japan. The genes related to fruiting body formation in the basidiomycete were identified by whole-genome analysis.

Some properties of flammutoxin from the edible mushroom Flammulina velutipes

Toxicon ◽  
1987 ◽  
Vol 25 (11) ◽  
pp. 1145-1152 ◽  
Author(s):  
Alan W. Bernheimer ◽  
Joel D. Oppenheim
Keyword(s):  
Edible Mushroom ◽  
Flammulina Velutipes

scholarly journals Multiplex PCR for the Identification of Pathogenic Listeria in Flammulina velutipes Plant Based on Novel Specific Targets Revealed by Pan-Genome Analysis

2021 ◽  
Vol 11 ◽  
Author(s):  
Fan Li ◽  
Qinghua Ye ◽  
Moutong Chen ◽  
Jumei Zhang ◽  
Liang Xue ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genome Analysis ◽  
Detection Limits ◽  
Rapid Identification ◽  
Flammulina Velutipes ◽  
The Novel ◽  
Production Environment ◽  
Pan Genome ◽  
Listeria Ivanovii ◽  
Mpcr Assay

Listeria spp. is an important foodborne disease agent, often found in the fresh mushroom (Flammulina velutipes) and its production environment. The aim of this study was to develop multiplex PCR for rapid identification of Listeria monocytogenes and Listeria ivanovii, and nonpathogenic Listeria in F. velutipes plants. Pan-genome analysis was first used to identify five novel Listeria-specific targets: one for the Listeria genus, one for L. monocytogenes, and three for L. ivanovii. Primers for the novel targets were highly specific in individual reactions. The detection limits were 103–104 CFU/mL, meeting the requirements of molecular detection. A mPCR assay for the identification of pathogenic Listeria, with primers targeting the novel genes specific for Listeria genus (LMOSLCC2755_0944), L. monocytogenes (LMOSLCC2755_0090), and L. ivanovii (queT_1) was then designed. The assay specificity was robustly verified by analyzing nonpathogenic Listeria and non-Listeria spp. strains. The determined detection limits were 2.0 × 103 CFU/mL for L. monocytogenes and 3.4 × 103 CFU/mL for L. ivanovii, for pure culture analysis. Further, the assay detected 7.6 × 104 to 7.6 × 100 CFU/10 g of pathogenic Listeria spiked into F. velutipes samples following 4–12 h enrichment. The assay feasibility was evaluated by comparing with a traditional culture-based method, by analyzing 129 samples collected from different F. velutipes plants. The prevalence of Listeria spp. and L. monocytogenes was 58.1% and 41.1%, respectively. The calculated κ factors for Listeria spp., L. monocytogenes, and L. ivanovii were 0.97, 0.97, and 1, respectively. The results of the novel mPCR assay were highly consistent with those of the culture-based method. The new assay thus will allow rapid, specific, and accurate detection and monitoring of pathogenic Listeria in food and its production environment.

Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei

2012 ◽  
Vol 45 (1) ◽  
pp. 232-237 ◽  
Author(s):  
Angel B. Encarnacion ◽  
Fernand Fagutao ◽  
Orapint Jintasataporn ◽  
Wanchai Worawattanamateekul ◽  
Ikuo Hirono ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Penaeus Monodon ◽  
Edible Mushroom ◽  
Flammulina Velutipes

Two new sesquiterpenes and six norsesquiterpenes from the solid culture of the edible mushroom Flammulina velutipes

Tetrahedron ◽  
2012 ◽  
Vol 68 (14) ◽  
pp. 3012-3018 ◽  
Author(s):  
Yaqi Wang ◽  
Li Bao ◽  
Dailin Liu ◽  
Xiaoli Yang ◽  
Shaifei Li ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Flammulina Velutipes ◽  
Solid Culture
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