Azetidine-Bearing Non-Ribosomal Peptides, Bonnevillamides D and E, Isolated from a Carrion Beetle-Associated Actinomycete

2021 ◽  
Author(s):  
Yern-Hyerk Shin ◽  
Yeon Hee Ban ◽  
Jisu Shin ◽  
In Wook Park ◽  
Soljee Yoon ◽  
...  
Keyword(s):  
Carrion Beetle ◽  
Non Ribosomal Peptides

scholarly journals PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization

2021 ◽  
Author(s):  
Kenichi Matsuda ◽  
Kei Fujita ◽  
Toshiyuki Wakimoto
Keyword(s):  
Enzymatic Activity ◽  
Computational Model ◽  
Binding Protein ◽  
Penicillin Binding Protein ◽  
Small Peptide ◽  
Peptide Cyclization ◽  
Chain Lengths ◽  
Non Ribosomal Peptides

Abstract Penicillin binding protein-type thioesterases (PBP-type TEs) are a recently identified group of peptide cyclases that catalyze head-to-tail macrolactamization of non-ribosomal peptides. PenA, a new member of this group, is involved in the biosyntheses of cyclic pentapeptides. In this study, we demonstrated the enzymatic activity of PenA in vitro, and analyzed its substrate scope with a series of synthetic substrates. A comparison of the reaction profiles between PenA and SurE, a representative PBP-type TE, showed that PenA is more specialized for small peptide cyclization. A computational model provided a possible structural rationale for the altered specificity for substrate chain lengths.

Engineering Heterologous Hosts for the Enhanced Production of Non-ribosomal Peptides

2020 ◽  
Vol 25 (6) ◽  
pp. 795-809
Author(s):  
Komal Sharma ◽  
Mohammad Rifqi Ghiffary ◽  
Hyun Uk Kim ◽  
Sang Yup Lee
Keyword(s):  
Enhanced Production ◽  
Non Ribosomal Peptides

scholarly journals The tridecaptins: non-ribosomal peptides that selectively target Gram-negative bacteria

2021 ◽  
Author(s):  
Samantha J. Bann ◽  
Ross D. Ballantine ◽  
Stephen A. Cochrane
Keyword(s):  
Gram Negative Bacteria ◽  
Antibacterial Peptides ◽  
Gram Negative ◽  
Non Ribosomal Peptides

Tridecaptins are a re-emerging class of non-ribosomal antibacterial peptides (NRAPs) with potent activity against highly problematic strains of Gram-negative bacteria.

scholarly journals Effect of Granulometric Composition of the Soil on the Occurrence of Carrion Beetles (Coleoptera: Silphidae)

2021 ◽  
Vol 11 (3) ◽  
pp. 1017
Author(s):  
Karolina Konieczna ◽  
Zbigniew W. Czerniakowski ◽  
Małgorzata Szostek
Keyword(s):  
Significant Relationship ◽  
Granulometric Composition ◽  
Clay Fraction ◽  
Total Abundance ◽  
Habitat Types ◽  
Sand Fraction ◽  
Positive Correlation ◽  
Nicrophorus Vespilloides ◽  
Carrion Beetle ◽  
Carrion Beetles

The entomological material was collected in the years 2009–2012 and 2014 from 13 different habitat types from three localities in south-eastern Poland. In total, 11,095 Silphidae were collected. This study examined whether the percentage of individual soil granulometric fractions was significantly related to the total abundance of collected Silphidae and individual carrion beetle species. A positive correlation and a statistically significant correlation were found between the total number of specimens collected and the share of the mechanical fraction with a diameter of 0.05–0.002 mm (silt fraction). In three species, a statistically significant correlation was demonstrated between the number of collected Silphidae and the share of the mechanical fraction with a diameter of 2–0.05 mm (sand fraction). The two species Phosphuga atrata atrata and Nicrophorus vespilloides the correlation was positive. A statistically significant relationship was also observed for Thanatophilus sinuatus, but the correlation was negative. With regard to the mechanical fraction with a diameter <0.002 mm (clay fraction), a statistically significant relationship was demonstrated for Oiceoptoma thoracicum and for Nicrophorus vespilloides for which the correlation was negative. However, a positive correlation was found for T. sinuatus in this case.

Structures and Biosynthetic Pathway of Coprisamides C and D, 2-Alkenylcinnamic Acid-Containing Peptides from the Gut Bacterium of the Carrion Beetle Silpha perforata

2021 ◽  
Vol 84 (2) ◽  
pp. 239-246
Author(s):  
Yern-Hyerk Shin ◽  
Yeon Hee Ban ◽  
Tae Ho Kim ◽  
Eun Seo Bae ◽  
Jongheon Shin ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Carrion Beetle

scholarly journals Pentaminomycins F and G, First Non-Ribosomal Peptides Containing 2-Pyridylalanine

2020 ◽  
Author(s):  
Daniel Carretero Molina ◽  
Francisco Javier Ortiz-Lopez ◽  
Jesús Martín ◽  
Ignacio González ◽  
Marina Sánchez-Hidalgo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Peptide Synthetase ◽  
Non Ribosomal Peptides

Pentaminomycins F-H, a group of three new hydroxyarginine-containing cyclic pentapeptides, were isolated from cultures of a <i>Streptomyces cacaoi</i> subsp. <i>cacaoi</i> strain along with the known pentaminomycins A-E. The structures of the new peptides were determined by a combination of mass spectrometry and NMR and Marfey's analyses. Among them, pentaminomycins F and G were shown to contain in their structures the rare amino acid 3-(2-pyridyl)-alanine. This finding represents the first reported example of non-ribosomal peptides containing this residue. The LDLLD chiral sequence found for the three compounds was in agreement with that reported for previously isolated pentaminomycins and consistent with the epimerization domains present in the putative non-robosomal peptide synthetase (NRPS) biosynthetic gene cluster.<br>

scholarly journals Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Mark J. Calcott ◽  
Jeremy G. Owen ◽  
David F. Ackerley
Keyword(s):  
Amide Bond ◽  
Assembly Lines ◽  
Modular Assembly ◽  
Catalytic Domains ◽  
Peptide Modification ◽  
Proof Reading ◽  
Peptide Synthetase ◽  
A Domains ◽  
High Yields ◽  
Non Ribosomal Peptides

Abstract Non-ribosomal peptide synthetase (NRPS) enzymes form modular assembly-lines, wherein each module governs the incorporation of a specific monomer into a short peptide product. Modules are comprised of one or more key domains, including adenylation (A) domains, which recognise and activate the monomer substrate; condensation (C) domains, which catalyse amide bond formation; and thiolation (T) domains, which shuttle reaction intermediates between catalytic domains. This arrangement offers prospects for rational peptide modification via substitution of substrate-specifying domains. For over 20 years, it has been considered that C domains play key roles in proof-reading the substrate; a presumption that has greatly complicated rational NRPS redesign. Here we present evidence from both directed and natural evolution studies that any substrate-specifying role for C domains is likely to be the exception rather than the rule, and that novel non-ribosomal peptides can be generated by substitution of A domains alone. We identify permissive A domain recombination boundaries and show that these allow us to efficiently generate modified pyoverdine peptides at high yields. We further demonstrate the transferability of our approach in the PheATE-ProCAT model system originally used to infer C domain substrate specificity, generating modified dipeptide products at yields that are inconsistent with the prevailing dogma.

scholarly journals The complete mitochondrial genome of a potentially forensic related carrion beetle, Diamesus osculans (Vigors, 1825)

2020 ◽  
Vol 5 (2) ◽  
pp. 1423-1424
Author(s):  
Xiaoning Zhang ◽  
Qingbai Hou ◽  
Lei Zhang ◽  
Jifeng Cai ◽  
Fanming Meng
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Carrion Beetle

scholarly journals Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products inR. toruloidesto include non-ribosomal peptides

2019 ◽  
Vol 21 (12) ◽  
pp. 3394-3406 ◽  
Author(s):  
Maren Wehrs ◽  
John M. Gladden ◽  
Yuzhong Liu ◽  
Lukas Platz ◽  
Jan-Philip Prahl ◽  
...  
Keyword(s):  
Sustainable Production ◽  
Blue Pigment ◽  
Production Host ◽  
Non Ribosomal Peptides

Development ofR. toruloidesas a production host for the sustainable production of the NRP indigoidine.

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