Tetrahydrobenzofuran-6(2H)-one Neolignans from Ocotea heterochroma: Their Platelet Activating Factor (PAF) Antagonistic Activity and in Silico Insights into the PAF Receptor Binding Mode

2018 ◽  
Vol 81 (9) ◽  
pp. 1968-1975
Author(s):  
Catalina Rozo-Lugo ◽  
Luis Enrique Cuca-Suárez ◽  
Thomas J. Schmidt ◽  
Ericsson Coy-Barrera
Keyword(s):  
Receptor Binding ◽  
In Silico ◽  
Platelet Activating Factor ◽  
Binding Mode ◽  
Antagonistic Activity ◽  
Paf Receptor

scholarly journals An Antagonistic Activity of Etizolam on Platelet-Activating Factor (PAF) In Vitro Effects on Platelet Aggregation and PAF Receptor Binding

1987 ◽  
Vol 44 (4) ◽  
pp. 387-391
Author(s):  
Hiroshi MIKASHIMA ◽  
Shüzö TAKEHARA ◽  
Yoshito MURAMOTO ◽  
Takako KHOMARU ◽  
Michio TERASAWA ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Receptor Binding ◽  
Platelet Activating Factor ◽  
Antagonistic Activity ◽  
Paf Receptor ◽  
In Vitro Effects

scholarly journals An antagonistic activity of etizolam on platelet-activating factor(PAF). In vitro effects on platelet aggregation and PAF receptor binding.

1987 ◽  
Vol 44 (4) ◽  
pp. 387-391 ◽  
Author(s):  
Hiroshi MIKASHIMA ◽  
Shuzo TAKEHARA ◽  
Yoshito MURAMOTO ◽  
Takako KHOMARU ◽  
Michio TERASAWA ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Receptor Binding ◽  
Platelet Activating Factor ◽  
Antagonistic Activity ◽  
Paf Receptor ◽  
In Vitro Effects

Platelet-activating factor (PAF) receptor binding activity of the roots ofEnicosanthellum pulchrum

2011 ◽  
Vol 50 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Noraziah Nordin ◽  
Juriyati Jalil ◽  
Ibrahim Jantan ◽  
Shahnaz Murad
Keyword(s):  
Receptor Binding ◽  
Platelet Activating Factor ◽  
Binding Activity ◽  
Receptor Binding Activity ◽  
Paf Receptor

Platelet-activating factor (PAF) receptor binding antagonists from Alpinia officinarum

2007 ◽  
Vol 17 (24) ◽  
pp. 6720-6722 ◽  
Author(s):  
Gao-jun Fan ◽  
Young-Hwa Kang ◽  
Yong Nam Han ◽  
Byung Hoon Han
Keyword(s):  
Receptor Binding ◽  
Platelet Activating Factor ◽  
Alpinia Officinarum ◽  
Paf Receptor

Platelet-activating factor receptors in lamb lungs are downregulated immediately after birth

2000 ◽  
Vol 278 (4) ◽  
pp. H1168-H1176 ◽  
Author(s):  
Basil O. Ibe ◽  
Fred C. Sander ◽  
J. Usha Raj
Keyword(s):  
Receptor Binding ◽  
Dissociation Constants ◽  
Receptor Gene ◽  
Choline Chloride ◽  
Platelet Activating Factor ◽  
Scatchard Analysis ◽  
Newborn Period ◽  
Paf Receptor ◽  
G Protein Coupled ◽  
Receptor Gene Expression

Platelet-activating factor (PAF) is a phospholipid with diverse biological functions mediated by a G protein-coupled receptor. We determined PAF receptor binding in lung membranes of four groups of perinatal lambs. Membrane protein (100 μg/ml) was incubated for 60 min at 30°C with 0.5–24 nM of acetyl-[3H]PAF in 30 mM Tris buffer, pH 7.2, containing 0.25% BSA, 10 mM MgCl2, and 125 mM choline chloride. PAF bound to membrane was isolated and quantified by scintillation spectrometry, followed with Scatchard analysis for receptor density (Bmax). The Bmax (means ± SE, fmol/mg protein) were 445.8 ± 12.3, 244.2 ± 3.3, 250.6 ± 3.6, and 419.9 ± 8.6 for the fetal, 90-min-old, <1-day-old, and 6- to 12-day-old lambs, respectively. The Bmax for the 90-min-old and <1-day-old lambs were not different but were significantly lower than those of either the term fetal or 6- to 12-day-old lambs. These data show a significant decrease in PAF binding to its receptor and in PAF Bmax in lung membranes of immediate newborn lambs. The dissociation constants ( K D, nM) were 7.7 ± 0.52, 11.5 ± 0.34, 6.9 ± 0.48, and 5.0 ± 0.53 for fetal, 90-min-old, <1-day-old, and 6- to 12-day-old newborn lamb lungs, respectively. The K D of the 90-min-old lamb was the highest of all. PAF receptor gene measured by RT-PCR showed a significant downregulation of PAF receptor gene mRNA in lungs of lambs <1 day old, suggesting a transcriptional regulation of PAF receptor gene expression in the immediate newborn period. We speculate that decreased PAF receptor binding immediately after birth will facilitate the fall in pulmonary vascular resistance in the immediate newborn period.

ChemInform Abstract: Platelet-Activating Factor (PAF) Receptor Binding Antagonists from Alpinia officinarum.

ChemInform ◽  
2008 ◽  
Vol 39 (15) ◽  
Author(s):  
Gao-jun Fan ◽  
Young-Hwa Kang ◽  
Yong Nam Han ◽  
Byung Hoon Han
Keyword(s):  
Receptor Binding ◽  
Platelet Activating Factor ◽  
Alpinia Officinarum ◽  
Paf Receptor
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