All-Atom Simulations Reveal Protein Charge Decoration in the Folded and Unfolded Ensemble Is Key in Thermophilic Adaptation

2017 ◽  
Vol 13 (10) ◽  
pp. 5065-5075 ◽  
Author(s):  
Lucas Sawle ◽  
Jonathan Huihui ◽  
Kingshuk Ghosh
Keyword(s):  
Protein Charge ◽  
Thermophilic Adaptation

The Effects of Protein Charge Patterning on Complex Coacervation

2021 ◽  
Author(s):  
Nicholas Zervoudis ◽  
Allie Obermeyer
Keyword(s):  
Phase Boundary ◽  
Phase Behavior ◽  
Predictive Models ◽  
Phase Portraits ◽  
Complex Coacervation ◽  
Protein Encapsulation ◽  
Protein Charge ◽  
Polypeptide Sequence ◽  
The Impact ◽  
Coacervate Phase

The complex coacervation of proteins with other macromolecules has applications in protein encapsulation and delivery and for determining the function of cellular coacervates. Theoretical or empirical predictions for protein coacervates would enable the design of these coacervates with tunable and predictable structure-function relationships; unfortunately, no such theories exist. To help establish predictive models, the impact of protein-specific parameters on complex coacervation were probed in this study. The complex coacervation of sequence-specific, polypeptide-tagged, GFP variants and a strong synthetic polyelectrolyte was used to evaluate the effects of protein charge patterning on phase behavior. Phase portraits for the protein coacervates demonstrated that charge patterning dictates the protein’s binodal phase boundary. Protein concentrations over 100 mg mL<sup>-1</sup> were achieved in the coacervate phase, with concentrations dependent on the polypeptide sequence. In addition to shifting the binodal phase boundary, polypeptide charge patterning provided entropic advantages over isotropically patterned proteins. Together, these results show that modest changes of only a few amino acids alter the coacervation thermodynamics and can be used to tune the phase behavior of polypeptides or proteins of interest.

Protein Purification by Polyelectrolyte Coacervation: Influence of Protein Charge Anisotropy on Selectivity

2011 ◽  
Vol 12 (5) ◽  
pp. 1512-1522 ◽  
Author(s):  
Yisheng Xu ◽  
Malek Mazzawi ◽  
Kaimin Chen ◽  
Lianhong Sun ◽  
Paul L. Dubin
Keyword(s):  
Protein Purification ◽  
Protein Charge

scholarly journals Protein and DNA sequence determinants of thermophilic adaptation

2005 ◽  
Vol preprint (2006) ◽  
pp. e5
Author(s):  
Konstantin B Zeldovich ◽  
Igor N Berezovsky ◽  
Eugene I. Shakhnovich
Keyword(s):  
Dna Sequence ◽  
Thermophilic Adaptation

scholarly journals Protein charge distribution in proteomes and its impact on translation

2017 ◽  
Vol 13 (5) ◽  
pp. e1005549 ◽  
Author(s):  
Rodrigo D. Requião ◽  
Luiza Fernandes ◽  
Henrique José Araujo de Souza ◽  
Silvana Rossetto ◽  
Tatiana Domitrovic ◽  
...  
Keyword(s):  
Charge Distribution ◽  
Protein Charge

scholarly journals TKSA-MC: A web server for rational mutation through the optimization of protein charge interactions

2018 ◽  
Vol 86 (11) ◽  
pp. 1184-1188 ◽  
Author(s):  
Vinícius G. Contessoto ◽  
Vinícius M. de Oliveira ◽  
Bruno R. Fernandes ◽  
Gabriel G. Slade ◽  
Vitor B. P. Leite
Keyword(s):  
Web Server ◽  
Protein Charge ◽  
Charge Interactions

scholarly journals Protein Charge and Ion Concentration Affect Protein Diffusion in the Prokaryotic Cytoplasm

2017 ◽  
Vol 112 (3) ◽  
pp. 240a
Author(s):  
Wojciech M. Smigiel ◽  
Paul E. Schavemaker ◽  
Bert Poolman
Keyword(s):  
Ion Concentration ◽  
Protein Diffusion ◽  
Protein Charge
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