Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk

2020 ◽  
Vol 68 (4) ◽  
pp. 1118-1125 ◽  
Author(s):  
Jinmei Fu ◽  
Yaofeng Zhou ◽  
Xiaolin Huang ◽  
Wenjing Zhang ◽  
Yuhao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Immunochromatographic Assay ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

The PCR-HCR dual signal amplification strategy for ultrasensitive detection of Escherichia coli O157:H7 in milk

LWT ◽  
2020 ◽  
Vol 130 ◽  
pp. 109642 ◽  
Author(s):  
Taobo Liang ◽  
Xin Wu ◽  
Bolu Chen ◽  
Ju Liu ◽  
Zoraida P. Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157 ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62300-62305 ◽  
Author(s):  
Jing-Yun Wang ◽  
Ming-Hui Chen ◽  
Zhi-Chao Sheng ◽  
Dao-Feng Liu ◽  
Song-Song Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Test Strip ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7.

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

Rapid and Sensitive Detection of Escherichia coli O157:H7 in Milk and Ground Beef Using Magnetic Bead–Based Immunoassay Coupled with Tyramide Signal Amplification

2014 ◽  
Vol 77 (1) ◽  
pp. 100-105 ◽  
Author(s):  
MUHSIN AYDIN ◽  
GENE P. D. HERZIG ◽  
KWANG CHEOL JEONG ◽  
SAMANTHA DUNIGAN ◽  
PARTH SHAH ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Food Safety ◽  
Foodborne Pathogens ◽  
Signal Amplification ◽  
Magnetic Bead ◽  
Escherichia Coli O157 ◽  
Tyramide Signal Amplification ◽  
E Coli ◽  
Enrichment Step

Escherichia coli O157:H7 is a major foodborne pathogen that has posed serious problems for food safety and public health. Recent outbreaks and recalls associated with various foods contaminated by E. coli O157:H7 clearly indicate its deleterious effect on food safety. A rapid and sensitive detection assay is needed for this harmful organism to prevent foodborne illnesses and control outbreaks in a timely manner. We developed a magnetic bead–based immunoassay for detection of E. coli O157:H7 (the most well-known Shiga toxigenic E. coli strain) with a 96-well microplate as an assay platform. Immunomagnetic separation (IMS) and tyramide signal amplification were coupled to the assay to increase its sensitivity and specificity. This immunoassay was able to detect E. coli O157:H7 in pure culture with a detection limit of 50 CFU/ml in less than 3 h without an enrichment step. The detection limit was decreased 10-fold to 5 CFU/ml with addition of a 3-h enrichment step. When this assay was tested with other nontarget foodborne pathogens and common enteric bacteria, no cross-reactivity was found. When tested with artificially contaminated ground beef and milk samples, the assay sensitivity decreased two- to fivefold, with detection limits of 250 and 100 CFU/ml, respectively, probably because of the food matrix effect. The assay results also were compared with those of a sandwich-type enzyme-linked immunosorbent assay (ELISA) and an ELISA coupled with IMS; the developed assay was 25 times and 4 times more sensitive than the standard ELISA and the IMS-ELISA, respectively. Tyramide signal amplification combined with IMS can improve sensitivity and specificity for detection of E. coli O157:H7. The developed assay could be easily adapted for other foodborne pathogens and will contribute to improved food safety and public health.

Poly-L-lysine brushes on magnetic nanoparticles for ultrasensitive detection of Escherichia coli O157: H7

Talanta ◽  
2017 ◽  
Vol 172 ◽  
pp. 53-60 ◽  
Author(s):  
Jing Wu ◽  
Yan Chen ◽  
Yu Wang ◽  
Hong Yin ◽  
Zhitao Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Magnetic Nanoparticles ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157
Sign in / Sign up

Export Citation Format

Share Document