Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification

2018 ◽  
Vol 66 (19) ◽  
pp. 4941-4947 ◽  
Author(s):  
Seong U. Kim ◽  
Eun-Jung Jo ◽  
Hyoyoung Mun ◽  
Yuseon Noh ◽  
Min-Gon Kim
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Immunomagnetic Separation ◽  
Nitroblue Tetrazolium ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157 ◽  
Selective Filtration

The PCR-HCR dual signal amplification strategy for ultrasensitive detection of Escherichia coli O157:H7 in milk

LWT ◽  
2020 ◽  
Vol 130 ◽  
pp. 109642 ◽  
Author(s):  
Taobo Liang ◽  
Xin Wu ◽  
Bolu Chen ◽  
Ju Liu ◽  
Zoraida P. Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157 ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

Food Control ◽  
2009 ◽  
Vol 20 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Belgin Sarimehmetoglu ◽  
Mihriban Hatun Aksoy ◽  
Naim Deniz Ayaz ◽  
Yildiz Ayaz ◽  
Ozlem Kuplulu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Ground Beef ◽  
Immunomagnetic Separation ◽  
Escherichia Coli O157

Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62300-62305 ◽  
Author(s):  
Jing-Yun Wang ◽  
Ming-Hui Chen ◽  
Zhi-Chao Sheng ◽  
Dao-Feng Liu ◽  
Song-Song Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Test Strip ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7.

Comparison of Rapid Enzyme-Linked Immunosorbent Assay and Immunomagnetic Separation Methods for Detection of Escherichia coli O157 in Fecal, Hide, Carcass, and Ground Beef Samples

2007 ◽  
Vol 70 (10) ◽  
pp. 2230-2234 ◽  
Author(s):  
T. W. THOMPSON ◽  
T. P. STEPHENS ◽  
G. H. LONERAGAN ◽  
M. F. MILLER ◽  
M. M. BRASHEARS
Keyword(s):  
Escherichia Coli ◽  
Ground Beef ◽  
Immunomagnetic Separation ◽  
Separation Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Escherichia Coli O157 ◽  
Perfect Agreement ◽  
Fecal Samples ◽  
E Coli ◽  
Beef Products

Rapid enzyme-linked immunosorbent assays (ELISAs) are approved for detection of Escherichia coli O157 in beef products. However, these kits have also been used in the industry to detect this pathogen on hides or in feces of cattle, although this use has not been validated. The objective of this study was to compare commercially available ELISAs (E. coli Now, Reveal, and VIP) with immunomagnetic separation along with selective media to detect E. coli O157 on hides, in feces, and in medium- and low-level-inoculated ground beef and carcasses (simulated by using briskets) samples. Naturally infected hide and fecal samples were subjected to both the immunomagnetic separation method and ELISAs for the detection of E. coli O157. Additionally, E. coli O157 inoculated and noninoculated ground beef and beef briskets were used to simulate meat and carcass samples. When comparing the detection results from the ELISAs (E. coli Now, Reveal, and VIP) to the immunomagnetic separation method, poor agreement was observed for fecal samples (kappa = 0.10, 0.02, and 0.03 for E. coli Now, Reveal, and VIP, respectively), and fair-to-moderate agreement was observed for hide samples (kappa = 0.30, 0.51, and 0.29 for E. coli Now, Reveal, and VIP, respectively). However, there was near-perfect agreement between the immunomagnetic separation method and ELISAs for ground beef (kappa = 1, 1, and 0.80 for E. coli Now, Reveal, and VIP, respectively) and brisket (kappa = 1, 1, and 1 for E. coli Now, Reveal, and VIP, respectively) samples. Assuming immunomagnetic separation is the best available method, these data suggest that the ELISAs are not useful in detecting E. coli O157 from hide or fecal samples. However, when ELISAs are used on ground beef and beef brisket samples they can be used with a high degree of confidence.

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

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