Elevated β-Carotene Synthesis by the Engineered Rhodobacter sphaeroides with Enhanced CrtY Expression

2019 ◽  
Vol 67 (34) ◽  
pp. 9560-9568 ◽  
Author(s):  
Shan Qiang ◽  
An Ping Su ◽  
Ying Li ◽  
Zhi Chen ◽  
Ching Yuan Hu ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Β Carotene ◽  
Carotene Synthesis

Effect of Aeration on the Production of Carotenoid Pigments by Rhodotorula rubra-lactobacillus casei Subsp. casei Co-Cultures in Whey Ultrafiltrate

2003 ◽  
Vol 58 (3-4) ◽  
pp. 225-229 ◽  
Author(s):  
Emilina D. Simova ◽  
Ginka I. Frengova ◽  
Dora M. Beshkova
Keyword(s):  
Maximum Concentration ◽  
Lactobacillus Casei ◽  
Cheese Whey ◽  
Culture Fluid ◽  
Carotenoid Pigments ◽  
Air Flow Rate ◽  
Rhodotorula Rubra ◽  
Total Carotenoids ◽  
Β Carotene ◽  
Carotene Synthesis

Under intensive aeration (1.3 l/l min) the associated growth of Rhodotorula rubra GED2 and Lactobacillus casei subsp. casei in cheese whey ultrafiltrate (55 g lactose/l) proceeded effectively for both cultures with production of maximum carotenoids (12.4 mg/l culture fluid). For maximum amount of carotenoids synthesized in the cell, the yeast required more intensive aeration than the aeration needed for synthesis of maximum concentration of dry cells. Maximum concentration of carotenoids in the cell (0.49 mg/g dry cells) was registered with air flow rate at 1.3 l/l min, and of dry cells (27.0 g/l) at 1.0 l/l min. An important characteristic of carotenogenesis by Rhodotorula rubra GED2 + Lactobacillus casei subsp. casei was established - the intensive aeration (above 1.0 l/l min) stimulated β-carotene synthesis (60% of total carotenoids).

Biosynthese von Carotinoiden bei Chlorella

1957 ◽  
Vol 12 (6) ◽  
pp. 401-407 ◽  
Author(s):  
Hedwig Claes
Keyword(s):  
Chlorophyll Synthesis ◽  
Mutant Strains ◽  
Carotenoid Synthesis ◽  
Β Carotene ◽  
Carotene Synthesis

One of the recently described mutant strains of chlorella (strain 5/520) accumulates -in the dark phytoene, phytofluene, ζ-carotene, protetrahydrolycopene and prolycopene and forms α- and β-carotene, xanthophylls and appreciable amounts of chlorophyll in light only 1, 2.The experiments described below indicate that1. O2 and light are indispensable for xanthophyll and appreciable chlorophyll synthesis. Both syntheses appear to be linked somehow.2. Carotene synthesis depends upon light but not on O2 and is not linked to xanthophyll synthesis.3. Carotenoid synthesis in light is always accompanied by a decrease of polyenes of the Porter-Lincoln sequence, which have been accumulated in the cells during previous cultivation in the dark. Evidence seems strong that these polyenes are direct precursors of the normal carotenoids.4. An as yet unidentified carotene is formed in appreciable amounts along with the normal carotenes in the light, probably as byproduct, not a precursor.

scholarly journals Genetic Stability Developed for β-Carotene Synthesis in BR29 Rice Line Using Dihaploid Homozygosity

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100212 ◽  
Author(s):  
Karabi Datta ◽  
Gayetri Sahoo ◽  
Sellappan Krishnan ◽  
Moumita Ganguly ◽  
Swapan K. Datta
Keyword(s):  
Genetic Stability ◽  
Rice Line ◽  
Β Carotene ◽  
Carotene Synthesis

scholarly journals Enhanced β-carotene production by promoting the multivesicular body (MVB) pathway in Yarrowia lipolytica

2020 ◽  
Author(s):  
Fan Yang ◽  
Liang Liu ◽  
Shan Qiang ◽  
Ching Yuan Hu ◽  
Ying Li ◽  
...  
Keyword(s):  
Protein Level ◽  
Metabolic Pathways ◽  
Multivesicular Body ◽  
Carotene Content ◽  
Key Enzymes ◽  
New Strategy ◽  
Key Genes ◽  
Complex Regulatory Network ◽  
Β Carotene ◽  
Carotene Synthesis

Abstract Background: β-carotene is a precursor of vitamin A and has great commercial value as an additive in foods and feeds. Many pathways not directly related to the β-carotene synthesis affect β-carotene production since the interactions among metabolic fluxes of cells confer a complex regulatory network. Engineered Y. lipolytica strain has excellent potential for β-carotene production as oleaginous yeast. Optimizing indirectly metabolic pathways in Y. lipolytica may offer a new strategy for making the β-carotene production achieve a commercially viable yield.Results: In this study, we found that the proper promotion of the multivesicular body (MVB) sorting pathway elevated the production of β-carotene by 1.58 fold when overexpressing one copy of the Did2 gene in Y. lipolytica. Through the measurement of ATP, NADPH, the mRNA, and protein level of key genes in the β-carotene synthesis pathway, the reason for β-carotene elevated was deuced that the protein level of the key enzymes (tHMG and CarA) was increased. When overexpressing two copies of the Did2 gene, the transcription level of the key genes was all elevated. However, the protein level of key enzymes in the β-carotene synthesis pathway was reduced compared with the overexpressing one copy of the Did2 gene, which resulted in reduced β-carotene content.Conclusion: This study suggests that the MVB sorting pathway is not responsible for sorting protein but has a crucial regulating effect on protein abundance in cells. Engineering the MVB sorting pathway could potentially increase the production of other high-value products. Moreover, manipulation of indirectly related metabolic pathways also is a critical strategy in synthetic biology research.

scholarly journals Enhancement of β-Carotene Synthesis by Citrus Products

1963 ◽  
Vol 11 (2) ◽  
pp. 128-131
Author(s):  
Alex Ciegler ◽  
George E. N. Nelson ◽  
Harlow H. Hall
Keyword(s):  
Β Carotene ◽  
Carotene Synthesis

scholarly journals Enhancement of β-Carotene Synthesis by Citrus Products

1963 ◽  
Vol 11 (2) ◽  
pp. 128-131 ◽  
Author(s):  
Alex Ciegler ◽  
George E. N. Nelson ◽  
Harlow H. Hall
Keyword(s):  
Β Carotene ◽  
Carotene Synthesis

Metabolic regulation of α-linolenic acid on β-carotene synthesis in Blakeslea trispora revealed by a GC-MS-based metabolomic approach

RSC Advances ◽  
2015 ◽  
Vol 5 (78) ◽  
pp. 63193-63201 ◽  
Author(s):  
Jing Hu ◽  
Hao Li ◽  
Yumeng Yang ◽  
Shizeng Wang ◽  
Pingwah Tang ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Linolenic Acid ◽  
Metabolic Regulation ◽  
Blakeslea Trispora ◽  
Acetyl Coa ◽  
Β Carotene ◽  
Metabolomic Approach ◽  
Carotene Synthesis

ALA promoted β-carotene production in two ways: increasing the dissolved oxygen and decreasing the consumption of acetyl-CoA.

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