Metabolic regulation of α-linolenic acid on β-carotene synthesis in Blakeslea trispora revealed by a GC-MS-based metabolomic approach

RSC Advances ◽  
2015 ◽  
Vol 5 (78) ◽  
pp. 63193-63201 ◽  
Author(s):  
Jing Hu ◽  
Hao Li ◽  
Yumeng Yang ◽  
Shizeng Wang ◽  
Pingwah Tang ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Linolenic Acid ◽  
Metabolic Regulation ◽  
Blakeslea Trispora ◽  
Acetyl Coa ◽  
Β Carotene ◽  
Metabolomic Approach ◽  
Carotene Synthesis

ALA promoted β-carotene production in two ways: increasing the dissolved oxygen and decreasing the consumption of acetyl-CoA.

scholarly journals Natural β-Carotene Production by Blakeslea trispora Cultivated in Spanish-Style Green Olive Processing Wastewaters

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 327
Author(s):  
Eugenia Papadaki ◽  
Fani Th Mantzouridou
Keyword(s):  
Production Costs ◽  
Carotenoid Content ◽  
Blakeslea Trispora ◽  
Total Carotenoid ◽  
Total Carotenoid Content ◽  
Reference Medium ◽  
Green Olive ◽  
First Time ◽  
Β Carotene ◽  
Carotene Synthesis

In the current research, the potential of Spanish-style green olive processing wastewaters (lye and washing waters) exploitation toward natural β-carotene production by Blakeslea trispora was tested for the first time. Mating culture generated by the joint cultivation of the heterothallic fungal strains ATCC 14271 and 14272 in the non-sterile lye and washing waters was able to grow, achieving the phytotoxic hydroxytyrosol degradation by 57.3% and 66.8%, respectively. However, the low sugar and nitrogen content of the streams did not favor carotenogenesis. Alternatively, in the nutrient-enriched effluents, a notable quantity of β-carotene was produced, accounted for 61.2 mg/L (lye) and 64.1 mg/L (washing waters) (82–88% of total carotenoid content). Above all, enriched streams had a noteworthy stimulating effect on the β-carotene synthesis, because both the maximum β-carotene yield per volume of enriched effluents and specific β-carotene production rate were higher when compared with the respective values obtained from trials with synthetic reference medium without added effluents. Hydroxytyrosol and tyrosol showed high stability during the non-sterile process for β-carotene production by B. trispora grown in the enriched effluents. This finding strengthens the potential toward the generation of multiple high-value products, which could lower the natural β-carotene production costs.

Abstract 3560: Use of Hyperpolarized Magnetic Resonance for Non-Invasive Observation of Metabolic Regulation

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Marie A Schroeder ◽  
Lisa C Heather ◽  
Helen J Atherton ◽  
Kieran Clarke ◽  
George K Radda ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Metabolic Regulation ◽  
Krebs Cycle ◽  
Enzyme Regulation ◽  
Product Inhibition ◽  
Acetyl Coa ◽  
Fed State ◽  
Fasted Rats ◽  
Pyruvate Ratio

Hyperpolarized magnetic resonance (HP MR) has enabled real time visualization of in vivo metabolism. In this study, we postulated that HP MR could also non-invasively provide a measure of metabolic regulation. We focused on regulation of pyruvate dehydrogenase (PDH), a highly controlled enzyme that catalyzes the oxidation of pyruvate to acetyl CoA and CO2/HCO3-. We compared PDH flux in conditions of normal and attenuated enzyme activity, and in the presence of normal and augmented Krebs cycle flux, to determine the contributions of PDH activity and end product inhibition to enzyme regulation. Six rats were examined in the fed and fasted states (to modulate PDH activity), with 40 μmol HP 13C1-pyruvate alone and 40 μmol HP pyruvate co-infused with 40 μmol malate (to manipulate Krebs cycle flux/acetyl CoA uptake). HP tracer was infused into the rats in an MR scanner and cardiac spectra were acquired every second for 1 min. Conversion of pyruvate to 13HCO3-was monitored and the 13HCO3-/pyruvate ratio was used as a marker of PDH flux. Infusion of malate increased PDH flux by 31% compared with pyruvate alone, indicating that removal of acetyl CoA by incorporation into the Krebs cycle increased PDH flux. PDH flux was 57% lower in fasted rats injected with pyruvate alone compared with fed rats, and did not change with malate co-infusion. Here, low PDH activity prevented additional enzyme flux. These results suggest that end product inhibition limits fed state PDH flux, whereas PDH activity regulates pyruvate oxidation in the fasted state. In conclusion, this study has provided evidence that HP MR may be useful to obtain details of metabolic regulation, rather than just reflecting metabolic state. Figure 1 Bicarbonate/pyruvate ratio in fed and fasted rats, following an injection of pyruate or pyruate plus malate. In fed rats, co-infusion of malate increased PDH flux by 31% compared with injection of pyruvate alone (*p=0.02). Fasting reduced PDH flux by 57% (**p=0.002) following injection of pyruvate alone. Co-infusion with malate did not affect PDH flux in fasted rats.

Effect of Aeration on the Production of Carotenoid Pigments by Rhodotorula rubra-lactobacillus casei Subsp. casei Co-Cultures in Whey Ultrafiltrate

2003 ◽  
Vol 58 (3-4) ◽  
pp. 225-229 ◽  
Author(s):  
Emilina D. Simova ◽  
Ginka I. Frengova ◽  
Dora M. Beshkova
Keyword(s):  
Maximum Concentration ◽  
Lactobacillus Casei ◽  
Cheese Whey ◽  
Culture Fluid ◽  
Carotenoid Pigments ◽  
Air Flow Rate ◽  
Rhodotorula Rubra ◽  
Total Carotenoids ◽  
Β Carotene ◽  
Carotene Synthesis

Under intensive aeration (1.3 l/l min) the associated growth of Rhodotorula rubra GED2 and Lactobacillus casei subsp. casei in cheese whey ultrafiltrate (55 g lactose/l) proceeded effectively for both cultures with production of maximum carotenoids (12.4 mg/l culture fluid). For maximum amount of carotenoids synthesized in the cell, the yeast required more intensive aeration than the aeration needed for synthesis of maximum concentration of dry cells. Maximum concentration of carotenoids in the cell (0.49 mg/g dry cells) was registered with air flow rate at 1.3 l/l min, and of dry cells (27.0 g/l) at 1.0 l/l min. An important characteristic of carotenogenesis by Rhodotorula rubra GED2 + Lactobacillus casei subsp. casei was established - the intensive aeration (above 1.0 l/l min) stimulated β-carotene synthesis (60% of total carotenoids).

Biosynthese von Carotinoiden bei Chlorella

1957 ◽  
Vol 12 (6) ◽  
pp. 401-407 ◽  
Author(s):  
Hedwig Claes
Keyword(s):  
Chlorophyll Synthesis ◽  
Mutant Strains ◽  
Carotenoid Synthesis ◽  
Β Carotene ◽  
Carotene Synthesis

One of the recently described mutant strains of chlorella (strain 5/520) accumulates -in the dark phytoene, phytofluene, ζ-carotene, protetrahydrolycopene and prolycopene and forms α- and β-carotene, xanthophylls and appreciable amounts of chlorophyll in light only 1, 2.The experiments described below indicate that1. O2 and light are indispensable for xanthophyll and appreciable chlorophyll synthesis. Both syntheses appear to be linked somehow.2. Carotene synthesis depends upon light but not on O2 and is not linked to xanthophyll synthesis.3. Carotenoid synthesis in light is always accompanied by a decrease of polyenes of the Porter-Lincoln sequence, which have been accumulated in the cells during previous cultivation in the dark. Evidence seems strong that these polyenes are direct precursors of the normal carotenoids.4. An as yet unidentified carotene is formed in appreciable amounts along with the normal carotenes in the light, probably as byproduct, not a precursor.

scholarly journals Genetic Stability Developed for β-Carotene Synthesis in BR29 Rice Line Using Dihaploid Homozygosity

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100212 ◽  
Author(s):  
Karabi Datta ◽  
Gayetri Sahoo ◽  
Sellappan Krishnan ◽  
Moumita Ganguly ◽  
Swapan K. Datta
Keyword(s):  
Genetic Stability ◽  
Rice Line ◽  
Β Carotene ◽  
Carotene Synthesis
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