Thermal and Photochemical Stability of Anthocyanins from Black Carrot, Grape Juice, and Purple Sweet Potato in Model Beverages in the Presence of Ascorbic Acid

2019 ◽  
Vol 67 (19) ◽  
pp. 5647-5660 ◽  
Author(s):  
Violaine Gérard ◽  
Emel Ay ◽  
Fabrice Morlet-Savary ◽  
Bernadette Graff ◽  
Christophe Galopin ◽  
...  
2019 ◽  
Vol 87 (3) ◽  
pp. 16
Author(s):  
Yudi Her Oktaviono ◽  
Makhyan Jibril Al-Farabi ◽  
Luh Oliva Saraswati Suastika ◽  
Febriyanti Hartono ◽  
Yanni Dirgantara ◽  
...  

Impairment of the endothelial progenitor cells (EPCs) ability to proliferate and migrate in the patients with coronary heart disease (CHD) is partly caused by oxidative stress. This research evaluates the effect of treatment with Ipomoea batatas L./purple sweet potato (PSP) extract and l-ascorbic acid on the proliferation and migration of impaired EPCs. EPCs were isolated from CHD patient’s peripheral blood. EPCs culture were cultivated and divided into control (untreated), PSP extract treatment (dose 1 and 25 μg/mL), and l-ascorbic acid treatment (dose 10 and 250 μg/mL) groups for 48 h. EPCs proliferation was analyzed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay, and migration was evaluated with the cell migration assay kit. Statistical tests were evaluated using SPSS 25.0. This research showed that EPCs proliferation and migration was significantly higher in all PSP extract and l-ascorbic acid treatment compared to the control (p < 0.001). EPCs migration on treatment with a PSP extract dose of 25 μg/mL was significantly higher compared to the treatment with l-ascorbic acid dose of 250 μg/mL (303,000 ± 1000 compared to 215,000 ± 3000 cells, p< 0.001). In conclusion, both treatments with PSP extract and l-ascorbic acid can improve the proliferation and migration of impaired EPCs. At the dose of 25 μg/mL, PSP extract seems to be superior to the l-ascorbic acid dose of 250 μg/mL to improve EPCs migration.


Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1536 ◽  
Author(s):  
Alexandra D. Frond ◽  
Cristian I. Iuhas ◽  
Ioana Stirbu ◽  
Loredana Leopold ◽  
Sonia Socaci ◽  
...  

Vegetables comprise a significant portion of our daily diet with their high content in nutrients including fiber, vitamins, minerals, as well as phenolic compounds. Vegetable consumption has been shown to be positively associated with the prevention of several degenerative diseases thanks to their bioactive compounds. Accordingly, five selected vegetables, namely, red chicory, red onion, eggplant, purple sweet potato, and black carrot were thoroughly assessed for their phenolic content in this study. For this purpose, the total phenolic and flavonoid content of these five vegetables and their antioxidant activities that are based on three common methods ABTS radical cation decolorization assay (ABTS), Cupric Ion Reducing Antioxidant Capacity (CUPRAC), and DPPH scavenging activity assay were determined. Additionally, HPLC-PDA/Electrospray ionization coupled with mass spectrometry (HPLC-PDA/-ESI+-MS)-based identification and quantification of the members belonging to polyphenols present in each vegetable were determined. Statistical correlations between antioxidant activities and the specific type of phenolic compounds, such as anthocyanins, flavonoids, anthocyanins, and phenolic acids were further elucidated. Phenolic acids (chlorogenic and syringic acids) were found to be the most abundant compounds that are present in all samples. Among the anthocyanins, cyaniding derivatives were present in all vegetables. In terms of their antioxidant activities, the analyzed vegetables were ranked as red chicory > purple sweet potato > black carrot > eggplant > red onion, in descending order. Superior antioxidant activities exhibited by red chicory and purple sweet potato were attributed to the high content of phenolic compounds, especially flavonols (quercetin-3,4-O-diglucoside) in red chicory and anthocyanins (peonidin-3-caffeoyl p-hydroxybenzoylsophoroside-5-glucoside) in purple sweet potato.


2014 ◽  
Vol 680 ◽  
pp. 32-37
Author(s):  
Ayende ◽  
Andi Rustandi ◽  
Johny Wahyuadi Soedarsono ◽  
Dedi Priadi ◽  
Sulistijono ◽  
...  

Utilization of ascorbic acid as corrosion inhibitor has several weaknesses. These weaknesses are easy to oxidize in solution form and reduction of its antioxidant properties due to heat, light, oxidizing agent, dissolve oxygen, and heavy metals. Purple sweet potato extract is an alternative green corrosion inhibitor with major contains of anthocyanin compound. Anthocyanin will withstand enzymatic reaction and oxidation process of ascorbic acid. Addition of purple sweet potato extract in ascorbic acid will enhance inhibition efficiency of steel compare to the used of ascorbic acid alone. Interaction of purple sweet potato extract with 0.01M ascorbic acid in 0.1M FeCl3environment was analyzed using FTIR spectroscopy. The result shows that interaction of purple sweet potato extract with ascorbic acid in 0.1M FeCl3build ability to form iron chelate.


2019 ◽  
Vol 86 ◽  
pp. 116-123 ◽  
Author(s):  
Xue-Jiao Xu ◽  
Sheng Fang ◽  
Yan-Hua Li ◽  
Fan Zhang ◽  
Zhi-Peng Shao ◽  
...  

2014 ◽  
Vol 709 ◽  
pp. 384-389 ◽  
Author(s):  
Ayende ◽  
Andi Rustandi ◽  
Johny Wahyuadi Soedarsono ◽  
Dedi Priadi ◽  
Sulistijono ◽  
...  

Using vitamin C or ascorbic acid as corrosion inhibitor has several weakness. In its liquid form ascorbic acid is easily oxidized and its anti oxidant properties unstable due to heat, light, oxidizer, dissolved oxygen and heavy metals. Purple sweet potato (ipomoea batatas L) with its high anthocyanin is one of alternative for green corrosion inhibitor. Extract of purple sweet potato has the ability to hold the enzyme reaction and oxidation proses of ascorbic acid. Purple sweet potato extract mixed with ascorbid acid is used as inhibitor of API 5L steel in 3.5%NaCl. This compound is classifid as mixed type inhibitor. The adding of purple sweet potato extract will assist ascorbic acid to maintance its inhibition efficiency and increases surface layer corrosion resistant of steel. Polarization methods and electrocehmical impedance spectroscopy (EIS) are used to investigate corrosion rate and mechanism of the steel. Appliaction of 4 ml purple sweet potato mixed with 10-4M ascorbic acid inhibitor increases inhibition ability of API 5L steel from 23.5 to 57.52%. Purple sweet potato extract moves polarization curve to lower potential (below 82 mVolt) with tends to be more negative.


Author(s):  
Amran Laga ◽  
Tuflika Primi Putri ◽  
Adiansyah Syarifuddin ◽  
Nuril Hidayah ◽  
Muhpidah Muhpidah

Purple sweet potato starch is known for its anthocyanin content and has a high carbohydrate content, especially starch. Starch extraction in purple sweet potato can cause browning due to the presence of PPO enzymes which results in decreased anthocyanin content in the resulting starch. Ascorbic acid can be used as an anti-browning agent. The analysis results obtained are the drying of purple sweet potato starch can be done for 4 hours because at that time the water content has been constant for a long time of further drying. The anthocyanin content of purple sweet potato starch decreases with increasing concentration of the addition of ascorbic acid, but the addition of ascorbic acid causes an increase in antioxidants of purple sweet potato starch because ascorbic acid is one type of antioxidant


2013 ◽  
Vol 333-335 ◽  
pp. 1921-1925
Author(s):  
Lu Gao ◽  
Ke Da Li ◽  
Ying Chang Li

With catechol as a substrate, some kinetic parameters, including Michaelis constant (Km), maximum reaction velocity (Vmax) and substrate inhibition constant (KI) for the reaction catalyzed by polyphenol oxidase (PPO) from purple sweet potato (PSP) were mainly studied here by spectrophotometry. Kmand Vmaxwere determined depending on bi-reciprocal diagram of Lineweaver-Burk and Hanes-Woolf diagram respectively, with Kmof 12.06 mM and Vmaxof 43.66 mM·min. The effects of four various inhibitors on PPO activity were different. Ascorbic acid (AA) and phytic acid (PA) showed strong inhibitory effects, with AA of the highest effect and citric acid (CA) the lowest. Among these inhibitors, AA was a reversible competitive inhibitor with KIof 15.26 mM, which was significant and instructive to the quality and benefit improvement of processed PSP products.


2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins


2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.


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