scholarly journals Hapten Synthesis, Antibody Development, and a Highly Sensitive Indirect Competitive Chemiluminescent Enzyme Immunoassay for Detection of Dicamba

2019 ◽  
Author(s):  
Jingqian Huo ◽  
Bogdan Barnych ◽  
Zhenfeng Li ◽  
Debin Wan ◽  
Dongyang Li ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Highly Sensitive ◽  
Hapten Synthesis ◽  
Chemiluminescent Enzyme Immunoassay

Highly Sensitive Chemiluminescent Enzyme Immunoassay with Gelatin-Coated Ferrite Solid Phase

1994 ◽  
Vol 40 (9) ◽  
pp. 1830-1831 ◽  
Author(s):  
Mitsuo Isomura ◽  
Masayoshi Ueno ◽  
Kazuya Shimada ◽  
Hiroyuki Kogaki ◽  
Yoshihiro Ashihara
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Highly Sensitive ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Evaluation of the highly sensitive chemiluminescent enzyme immunoassay “Lumipulse HBsAg-HQ” for hepatitis B virus screening

2017 ◽  
Vol 32 (4) ◽  
pp. e22334 ◽  
Author(s):  
Matsuo Deguchi ◽  
Masanori Kagita ◽  
Nori Yoshioka ◽  
Hiroko Tsukamoto ◽  
Miyuki Takao ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Highly Sensitive ◽  
B Virus ◽  
Chemiluminescent Enzyme Immunoassay ◽  
Virus Screening

Highly sensitive phage-magnetic-chemiluminescent enzyme immunoassay for determination of zearalenone

2020 ◽  
Vol 325 ◽  
pp. 126905 ◽  
Author(s):  
Ruxia Liu ◽  
Ruirui Shi ◽  
Wenting Zou ◽  
Wenhua Chen ◽  
Xianchao Yin ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Highly Sensitive ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodies

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Minori Yamada ◽  
Akiko Eguchi ◽  
Koji Okuno ◽  
Koji Sakaguchi ◽  
Tetsuji Yamaguchi
Keyword(s):  
Enzyme Immunoassay ◽  
Detection Sensitivity ◽  
Amino Acid Residues ◽  
Cytokeratin 18 ◽  
E Coli ◽  
Coefficients Of Variation ◽  
Highly Sensitive ◽  
Clinical Diagnoses ◽  
Analysis Of Performance ◽  
Chemiluminescent Enzyme Immunoassay

AbstractFragmented cytokeratin 18 (fCK18) released from epithelial cells undergoing apoptosis is widely studied in various diseases. However, fCK18 measurement is not utilized in clinical practice due to imprecise disease-state cutoff values. Therefore, we set out to generate new monoclonal antibodies (mAbs) and a recombinant fCK18 (rfCK18) calibrator in an effort to develop a highly sensitive chemiluminescent enzyme immunoassay (CLEIA). New capture mAb (K18-624) had a high binding ability compared to the current commercial antibody. New detection mAb (K18-328) recognized 323S-340G of CK18. A rfCK18 was expressed in the soluble fraction of E. coli when the N-terminal region (260 amino acid residues) of CK18 was truncated. Analysis of performance and measurement of human fCK18 were evaluated using K18-624 and K18-328 in a highly sensitive CLEIA. The coefficients of variation (CV) for within-run and between-day repeatability were below 10% and the recoveries were in the range of 15%. The detection sensitivity was 0.056 ng/mL. Serum fCK18 levels were significantly increased in non-alcoholic steatohepatitis (NASH) patients when compared to healthy individuals. Our new fCK18 mAbs showed high affinity and sensitivity. CLEIA using our new antibodies will be useful in measuring fCK18 in human blood thereby generating accurate clinical diagnoses of human liver diseases.

A highly sensitive sandwich enzyme immunoassay of urinary growth hormone in children with short stature, Turner's syndrome, and simple obesity

1989 ◽  
Vol 121 (4) ◽  
pp. 513-519 ◽  
Author(s):  
Hiroshi Tomita ◽  
Masamichi Ogawa ◽  
Takashi Kamijo ◽  
Osamu Mori ◽  
Eiji Ishikawa ◽  
...  
Keyword(s):  
Short Stature ◽  
Enzyme Immunoassay ◽  
Gh Deficiency ◽  
Urine Samples ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Highly Sensitive ◽  
Significant Difference ◽  
Simple Obesity ◽  
Sandwich Enzyme Immunoassay

Abstract. GH values were determined by a highly sensitive sandwich enzyme immunoassay in the 1st morning and/or 24-h accumulated urine samples in 94 children (short stature 70, including 14 with complete GH deficiency, 9 with partial GH deficiency, and 47 with GH-normal short stature; Turner's syndrome, 10, and simple obesity, 14). GH values were also determined in the 2nd to 4th urine samples taken on the same day together with the 1st morning urine in 5 of them. GH values in the 1st morning urine correlated significantly with those of the 24-h urine and with serum peak and mean GH values during nocturnal sleep as a physiological GH secretion test. The 2nd to 4th urines had lower GH concentrations than the 1st morning urine. The GH value of the 1st morning urine in complete GH deficiency was significantly lower than those in GH-normal short stature, partial GH deficiency and Turner's syndrome. However, no significant difference was detected in urinary GH values between complete GH deficiency and simple obesity. We conclude that 1st morning urinary GH estimation may be useful for differentiation of complete GH deficiency from other causes of short stature, but may be difficult for the distinction between complete GH deficiency and obesity with normal GH secretory ability.

scholarly journals Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis

2021 ◽  
Vol 27 (6) ◽  
pp. 915-918 ◽  
Author(s):  
Toshiaki Ishii ◽  
Masakazu Sasaki ◽  
Kageto Yamada ◽  
Daiki Kato ◽  
Hiroyoshi Osuka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay
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