A Highly Sensitive and Specific Chemiluminescent Enzyme Immunoassay for Placental Alkaline Phosphatase in the Cerebrospinal Fluid of Patients with Intracranial Germinomas

2012 ◽  
Vol 48 (3) ◽  
pp. 141-145 ◽  
Author(s):  
Shinichiro Watanabe ◽  
Yasuo Aihara ◽  
Akira Kikuno ◽  
Toyoji Sato ◽  
Tsugikazu Komoda ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cerebrospinal Fluid ◽  
Enzyme Immunoassay ◽  
Placental Alkaline Phosphatase ◽  
Highly Sensitive ◽  
Chemiluminescent Enzyme Immunoassay

Chemiluminescent enzyme immunoassay of alpha-fetoprotein based on an adamantyl dioxetane phenyl phosphate substrate.

1989 ◽  
Vol 35 (12) ◽  
pp. 2319-2321 ◽  
Author(s):  
G H Thorpe ◽  
I Bronstein ◽  
L J Kricka ◽  
B Edwards ◽  
J C Voyta
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Immunoassay ◽  
Detection Limits ◽  
Alpha Fetoprotein ◽  
Phenyl Phosphate ◽  
Comparison Of Results ◽  
Enzyme Detection ◽  
Chemiluminescent Enzyme Immunoassay

Abstract We have evaluated a new chemiluminescent substrate for the alkaline phosphatase (EC 3.1.3.1) label used in a Hybritech Tandem-E immunoassay of alpha-fetoprotein (AFP). The new substrate, adamantyl 1,2-dioxetane phenyl phosphate (AMPPD), emits light at 477 nm when acted upon by the enzyme. Detection limits for AFP with this method were 33 ng/L (mean of 20 replicates of the zero standard + 2 SD) and 470 ng/L (twice background). Between-batch CVs ranged from 4.31% to 9.60% for AFP in the range 29.1-132.0 micrograms/L. Comparison of results for 49 specimens assayed with use of the chemiluminescent kit and a colorimetric version of the AFP assay gave statistical values as follows: slope = 0.88, intercept = 4.19, and r = 0.94.

scholarly journals Chemiluminescent Enzyme Immunoassay Using an Alkaline Phosphatase Label and an Indolyl Phosphate Substrate.

1994 ◽  
Vol 10 (5) ◽  
pp. 831-834 ◽  
Author(s):  
Takashi IKEGAMI ◽  
Hidetoshi ARAKAWA ◽  
Masako MAEDA ◽  
Akio TSUJI
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Precise detection of the germinomatous component of intracranial germ cell tumors of the basal ganglia and thalamus using placental alkaline phosphatase in cerebrospinal fluid

2021 ◽  
Author(s):  
Kentaro Chiba ◽  
Yasuo Aihara ◽  
Takakazu Kawamata
Keyword(s):  
Alkaline Phosphatase ◽  
Cerebrospinal Fluid ◽  
Basal Ganglia ◽  
Germ Cell ◽  
Sensitivity And Specificity ◽  
Germ Cell Tumors ◽  
Decisive Role ◽  
Target Tissue ◽  
Placental Alkaline Phosphatase ◽  
Intracranial Germ Cell Tumors

Abstract Purpose: The disadvantages of biopsy for lesions in the basal ganglia and thalamus include a risk of various complications, difficulty in selecting the target tissue in some cases due to indistinct neuroimaging findings and limited availability of sample tissue. Placental alkaline phosphatase (PLAP) plays a decisive role in the diagnosis and management of intracranial germ cell tumors (IGCTs) in the basal ganglia and thalamus. The present study aimed to demonstrate the ability, specificity, and optimal use of PLAP values obtained from cerebrospinal fluid (CSF).Methods: Twenty patients with lesions in the basal ganglia and thalamus were enrolled in this study: 11 had IGCTs and 9 had non-IGCTs. The values of PLAP and other established tumor markers in the CSF were measured in all patients before treatment.Results: The mean follow-up period was 76.0 months (range, 3–168) for all lesions. PLAP was elevated in all 11 patients with IGCTs in the basal ganglia or thalamus, whereas none of the patients with non-IGCT exhibited elevated PLAP. Thus, the sensitivity and specificity of PLAP were both 100%.Conclusion: Our data demonstrated that the PLAP value can specifically identify the germinomatous component even in cases of IGCTs in the basal ganglia or thalamus with high sensitivity and specificity. PLAP is undoubtedly beneficial for the safe and timely detection of the germinomatous component of IGCTs in the basal ganglia and thalamus, because reliance on PLAP measurement enables us to avoid invasive surgical procedures and facilitates the prompt initiation of chemoradiation therapy.

scholarly journals Excess EDTA interferes with cortisol measurement using a solid-phase, chemiluminescent enzyme immunoassay

2018 ◽  
Vol 30 (3) ◽  
pp. 438-441 ◽  
Author(s):  
Robert J. Kemppainen ◽  
Ellen N. Behrend ◽  
Stephanie F. Carter ◽  
Janeva E. Cole
Keyword(s):  
Alkaline Phosphatase ◽  
Plasma Concentration ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Serum Samples ◽  
Edta Plasma ◽  
Edta Concentration ◽  
Canine Serum ◽  
Chemiluminescent Enzyme Immunoassay

Hormone assays that use a solid-phase, automated, chemiluminescent enzyme immunoassay (CEIA) with an alkaline phosphatase–tagged hormone or antibody as a reporter are performed on serum or EDTA plasma in our laboratory. CEIA cortisol results appeared to increase in the presence of excess EDTA. We investigated the effect of the addition of different amounts of EDTA on cortisol concentrations in pooled canine serum samples. The recommended EDTA plasma concentration of 4.1 mmol/L (1.8 mg/mL) did not alter cortisol concentrations when added to serum pools; however, the addition of ≥5.1 mmol/L (2.25 mg/mL) of EDTA increased apparent concentrations of cortisol. Supplementation of serum samples with MgCl2 to 5 mmol/L reversed the effect of EDTA up to a concentration of ~8.1 mmol/L (3.6 mg/mL). Our findings show that CEIA cortisol results on EDTA plasma can be artificially increased if the EDTA concentration exceeds 5.1 mmol/L.

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