Effect of Poly(γ-glutamic acid) on the Physiological Responses and Calcium Signaling of Rape Seedlings (Brassica napusL.) under Cold Stress

2015 ◽  
Vol 63 (48) ◽  
pp. 10399-10406 ◽  
Author(s):  
Peng Lei ◽  
Zongqi Xu ◽  
Yan Ding ◽  
Bao Tang ◽  
Yunxia Zhang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Glutamic Acid ◽  
Calcium Signaling ◽  
Physiological Responses ◽  
Rape Seedlings

scholarly journals Investigating the physiological responses of Pacific white shrimp Litopenaeus vannamei to acute cold-stress

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7381 ◽  
Author(s):  
Zhenlu Wang ◽  
Yuexin Qu ◽  
Xiaolei Zhuo ◽  
Junyi Li ◽  
Jixing Zou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Litopenaeus Vannamei ◽  
Physiological Responses ◽  
Pacific White Shrimp ◽  
Basement Membranes ◽  
White Shrimp ◽  
Unfolded Protein ◽  
Activating Transcription Factor ◽  
The Unfolded Protein Response

Background Litopenaeus vannamei is one of the most important aquaculture shrimps in the world and low temperatures present a serious challenge to its survival, growth, and distribution. Methods To investigate their physiological responses during acute cold-stress, L. vannamei were treated under acute cooling conditions from 28 to 13 °C with a cooling rate of 2.5 °C/2 h and were maintained at 13 °C for 12 h. Plasma metabolite concentrations, histological changes, and relative gene expression related to the unfolded protein response (UPR) pathway and apoptosis in the hepatopancreas and the hemocytes of L. vannamei were investigated. Results The results revealed that the concentrations of triglycerides, total cholesterol, and total protein in plasma reached their peaks at 23 °C, and then decreased to their minimum values at 13 °C for 12 h. The activity of alkaline phosphatase in the plasma decreased to its lowest level while the activity of alanine aminotransferase increased to its highest level at 13 °C for 12 h. The hepatic tubules became necrotic and the basement membranes were ruptured at 13 °C for 12 h. The gene expression related to UPR and apoptosis in the hepatopancreas and hemocytes was significantly altered by the decrease in the temperature. Discussion The results revealed that acute cold-stress caused histological damage in the hepatopancreas of L. vannamei, reducing its immunity. The three UPR pathways were involved in the process of acute cold-stress and the response of activating transcription factor 6 to UPR may be faster and more directthan the IRE1 and PERK pathways.

scholarly journals Physiological Responses and Proteomic Analysis on the Cold Stress Responses of Annual Pitaya (Hylocereus spp.) Branches

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Junliang Zhou ◽  
Lijuan Wang ◽  
Tujian Xiao ◽  
Zhuang Wang ◽  
Yongya Mao ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Cold Resistance ◽  
Physiological Responses ◽  
Soluble Sugar ◽  
Multivariate Statistical ◽  
Chlorophyll Contents ◽  
Proteomic Approach ◽  
Related Proteins

In this study, the physiological response of the annual branches of three varieties of pitaya (Xianmi, Fulong, and Zihonglong) in cold stress was investigated using a multivariate statistical method. Physiological change results showed that cold stress could decrease the moisture and chlorophyll contents, on the contrary, increase the relative electric conductivity, the contents of malonadehyde, soluble protein, soluble sugar, and free proline, and enhance the enzyme activities of peroxidase, superoxide dismutase, and catalase. Meanwhile, a comparative proteomic approach was also conducted to clarify the cold resistance-related proteins and pathways in annual pitaya branches. Proteomics results concluded that the cold tolerance of annual pitaya branches could be improved by modulating autophagy. Therefore, we hypothesized that an increased autophagy ability may be an important characteristic of the annual pitaya branches in response to cold stress conditions. Our results provide a good understanding of the physiological responses and molecular mechanisms of the annual pitaya branches in response to cold stress.

scholarly journals Factors affecting resistance of winter rapeseed (Brassica napus L.) shoots to cold stress in vitro

2017 ◽  
Vol 24 (2) ◽  
Author(s):  
Remigijus Peleckis ◽  
Natalija Burbulis ◽  
Aušra Blinstrubienė ◽  
Vaida Jonytienė
Keyword(s):  
Cold Stress ◽  
Glutamic Acid ◽  
Electrolyte Leakage ◽  
Plant Biotechnology ◽  
Research Centre ◽  
Brassica Napus L ◽  
Joint Research ◽  
Factors Affecting ◽  
Winter Rapeseed

Research was carried out at the Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and at the Laboratory of Agrobiotechnology of the  Joint Research Centre. The effect of cultivar, acclimation duration and exogenous L-glutamic acid on the changes of proline level and electrolyte leakage in shoots of winter rapeseed was evaluated. The shoots were cultivated in the MS medium supplemented with 0, 1, 2, 3 and 4.0 mM l–1 L-glutamic acid. The resistance of shoots to cold stress (at –8 °C for 24 h) was estimated by an electrolyte leakage test. It was found that the exogenous L-glutamic acid stimulates the synthesis of endogenous proline in non-acclimated as well as acclimated shoots. The highest increasing in cold resistance of winter rapeseed shoots has been obtained under the  synergic effect of L-glutamic acid and acclimation. The shoots of cultivar ‘SY Kolumb’ were most cold-resistant independent of the acclimation duration and the concentration of L-glutamic acid in the medium.

scholarly journals Oxytocin Signaling Regulates the Homeostatic Response to Cold Stress in Poikilothermic Vertebrates

2021 ◽  
Author(s):  
Adi Segev ◽  
Shany Krispin ◽  
Anouk M Olthof ◽  
Katery Hyatt ◽  
Liran Haller ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Cold Stress ◽  
Physiological Responses ◽  
Metabolic Physiology ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Evolutionarily Conserved ◽  
Neuroendocrine Responses ◽  
Altered Metabolism ◽  
The Brain ◽  
Homeostatic Response

When exposed to low temperature, homeothermic vertebrates maintain internal body temperature by activating thermogenesis and by altered metabolism, synchronized by neuroendocrine responses. Although such physiological responses also occur in poikilothermic vertebrates, the prevailing notion is that their reactions are passive. Here, we explored molecular hypothalamic and physiological responses to cold stress in the tropical poikilotherm Nile tilapia (Oreochromis niloticus). We show that cold exposed tilapia exhibit complex homeostatic responses, including increased hypothalamic oxytocin, plasma glucose and cortisol concomitant with reduced plasma lactate and metabolic rate. Pharmacological or genetic blockage of oxytocin signaling further affected metabolic rate in two cold-exposed poikilothermic models. This indicates that oxytocin, a known thermoregulator in homeotherms, actively regulates temperature-related homeostasis in poikilotherms. Overall, our findings show that the brain of poikilotherms actively responds to cold temperature by regulating metabolic physiology. Moreover, we identify oxytocin signaling as an adaptive and evolutionarily conserved metabolic regulator of temperature-related homeostasis.

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