Gomisin J Inhibits Oleic Acid-Induced Hepatic Lipogenesis by Activation of the AMPK-Dependent Pathway and Inhibition of the Hepatokine Fetuin-A in HepG2 Cells

2015 ◽  
Vol 63 (44) ◽  
pp. 9729-9739 ◽  
Author(s):  
Myungsuk Kim ◽  
Sue Ji Lim ◽  
Hee-Ju Lee ◽  
Sun Young Kim ◽  
Chu Won Nho
Keyword(s):  
Oleic Acid ◽  
Hepg2 Cells ◽  
Hepatic Lipogenesis ◽  
Fetuin A ◽  
Dependent Pathway

Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD

2021 ◽  
Author(s):  
Jingda Li ◽  
Tianqi Wang ◽  
Panpan Liu ◽  
Fuyuan Yang ◽  
Xudong Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oleic Acid ◽  
Rat Model ◽  
High Fat Diet ◽  
Hepg2 Cells ◽  
Citrus Fruits ◽  
High Fat ◽  
Hepatic Oxidative Stress

Hesperetin as a major bioflavonoid in citrus fruits improves NAFLD by suppressing hepatic oxidative stress and inflammation.

Ellipticine induces apoptosis through p53-dependent pathway in human hepatocellular carcinoma HepG2 cells

Life Sciences ◽  
2006 ◽  
Vol 78 (22) ◽  
pp. 2550-2557 ◽  
Author(s):  
Yu-Chun Kuo ◽  
Po-Lin Kuo ◽  
Ya-Ling Hsu ◽  
Chien-Yu Cho ◽  
Chun-Ching Lin
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Human Hepatocellular Carcinoma ◽  
Dependent Pathway

scholarly journals Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

2021 ◽  
Vol 22 (16) ◽  
pp. 8847
Author(s):  
Fangfang Tie ◽  
Jin Ding ◽  
Na Hu ◽  
Qi Dong ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oleic Acid ◽  
Lipid Metabolism ◽  
Western Blot ◽  
Blot Analysis ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Western Blot Analysis ◽  
Heme Oxygenase 1 ◽  
And Oxidative Stress

Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases which lacks ideal treatment options. Kaempferol and kaempferide, two natural flavonol compounds isolated from Hippophae rhamnoides L., were reported to exhibit a strong regulatory effect on lipid metabolism, for which the mechanism is largely unknown. In the present study, we investigated the effects of kaempferol and kaempferide on oleic acid (OA)-treated HepG2 cells, a widely used in vitro model of NAFLD. The results indicated an increased accumulation of lipid droplets and triacylglycerol (TG) by OA, which was attenuated by kaempferol and kaempferide (5, 10 and 20 μM). Western blot analysis demonstrated that kaempferol and kaempferide reduced expression of lipogenesis-related proteins, including sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD-1). Expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer binding proteins β (C/EBPβ), two adipogenic transcription factors, was also decreased by kaempferol and kaempferide treatment. In addition, western blot analysis also demonstrated that kaempferol and kaempferide reduced expression of heme oxygenase-1 (HO-1) and nuclear transcription factor-erythroid 2-related factor 2 (Nrf2). Molecular docking was performed to identify the direct molecular targets of kaempferol and kaempferide, and their binding to SCD-1, a critical regulator in lipid metabolism, was revealed. Taken together, our findings demonstrate that kaempferol and kaempferide could attenuate OA-induced lipid accumulation and oxidative stress in HepG2 cells, which might benefit the treatment of NAFLD.

scholarly journals Antioxidant Capacity of Proteins and Hydrolysates from the Liver of Newborn Piglets, and Their Inhibitory Effects on Steatosis in vitro

2020 ◽  
Vol 58 (4) ◽  
Author(s):  
Ruilin Zhang ◽  
Lasheng Yin ◽  
Jian Chen ◽  
Xuewu Zhang
Keyword(s):  
Oleic Acid ◽  
Hepg2 Cells ◽  
Early Stage ◽  
Lipid Lowering ◽  
Inhibitory Effects ◽  
Hepatic Disorder ◽  
Newborn Piglets ◽  
End Stage ◽  
Lipid Lowering Effect

Research background. Non-alcoholic steatohepatitis is a potentially progressive hepatic disorder that can lead to end-stage liver disease and hepatocellular carcinoma. The inhibitory effects of proteins and hydrolysates from the liver of newborn piglets on hepatic steatosis in oleic-acid-induced HepG2 cells were investigated in vitro. Experimental approach. The extracted proteins from the liver of newborn piglets (NPLP) were hydrolysed with papain, pepsin, trypsin and Alcalase. Based on comparison of different enzyme digestion condition, a protein hydrolysis protocol was established to obtain hydrolysates with lipid-lowering effect. Results and conclusions. NPLH-trypsin (trypsin-digested NPLP hydrolysate) exhibited strong antioxidant activity and possessed good inhibitory effects on lipogenesis and cholesterol accumulation in HepG2 cells at 150 μg/mL, with a triglyceride decrease of (43±3) % and cholesterol decrease of (31±5) %, comparing with 0.75 mM oleic acid induced model. The addition of NPLH-trypsin (300 μg/mL) decreased alanine aminotransferase and aspartate aminotransferase activities and increased superoxide dismutase activity. Novelty and scientific contribution. This study demonstrated that NPLH-trypsin have a potential preventive effect on NAFLD in its early stage, and NPLH-trypsin has potential use as the modulator of lipid overaccumulation disease in food supplements.

scholarly journals Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Na Li ◽  
Yi-ran Sun ◽  
Li-bo He ◽  
Lei Huang ◽  
Ting-ting Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oleic Acid ◽  
Lipid Metabolism ◽  
Fatty Liver ◽  
Hepg2 Cells ◽  
Lipid Lowering ◽  
Stress System ◽  
Signal Pathways ◽  
Antioxidant Enzyme Activities ◽  
Nonalcoholic Fatty Liver

Idesia polycarpa Maxim. var. vestita Diels (I. polycarpa) is well known as an edible oil plant which contains abundant linoleic acid and polyphenols. The objective of this study was to maximize the by-product of defatted fruit of I. polycarpa. We found that the fraction D of ethyl acetate extract (EF-D) contained more polyphenols, which contribute to its strong antioxidant activity by antioxidant assays (DPPH, ABTS, and FRAP). Meanwhile, EF-D showed a significant lipid-lowering effect on oleic acid- (OA-) induced hepatic steatosis in HepG2 cells through enhancing antioxidant activity, reducing liver damage, and regulating lipid metabolism, antioxidant, and inflammation-related gene expression. The SOD and T-AOC levels significantly increased, but the levels of MDA, AST, and ALT decreased obviously when treated with EF-D. In general, EF-D improved the antioxidant enzyme activities and decreased the hepatic injury activities. Besides, treatment with EF-D for NAFLD influenced lipid metabolism and inflammation by activating PPARα which was associated with the increased expression of CPT1 and decreased expression of SCD, NF-κB, and IL-1. Moreover, EF-D improved the oxidative stress system through activation of the Nrf2 antioxidant signal pathways and upregulated its target genes of HO-1, NQO1, and GSTA2. The results highlighted the EF-D from the defatted fruit of I. polycarpa regarding lipid-lowering, proving it to be a potential drug resource of natural products for treating the nonalcoholic fatty liver disease (NAFLD).

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