3-Caffeoyl, 4-dihydrocaffeoylquinic acid from Salicornia herbacea attenuates high glucose-induced hepatic lipogenesis in human HepG2 cells through activation of the liver kinase B1 and silent information regulator T1/AMPK-dependent pathway

2013 ◽  
Vol 57 (3) ◽  
pp. 471-482 ◽  
Author(s):  
Yong Pil Hwang ◽  
Hyung Gyun Kim ◽  
Jae Ho Choi ◽  
Minh Truong Do ◽  
Thu Phuong Tran ◽  
...  
Keyword(s):  
High Glucose ◽  
Hepg2 Cells ◽  
Hepatic Lipogenesis ◽  
Liver Kinase B1 ◽  
Dependent Pathway ◽  
Salicornia Herbacea

scholarly journals Nobiletin Inhibits Hepatic Lipogenesis via Activation of AMP-Activated Protein Kinase

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Taewon Yuk ◽  
Younghwa Kim ◽  
Jinwoo Yang ◽  
Jeehye Sung ◽  
Heon Sang Jeong ◽  
...  
Keyword(s):  
Protein Kinase ◽  
High Glucose ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Hepatic Lipogenesis ◽  
Nonalcoholic Fatty Liver ◽  
Compound C ◽  
Amp Activated Protein Kinase

We aimed to investigate the effects of nobiletin on hepatic lipogenesis in high glucose-induced lipid accumulation in HepG2 cells. Nobiletin, a citrus polymethoxyflavonoid with six methoxy groups, is present abundantly in the peels of citrus fruits. HepG2 cells were incubated in Dulbecco’s modified Eagle’s medium containing high glucose (25 mM) and subsequently treated with nobiletin at different concentrations (5, 25, and 50 μM). Results showed that nobiletin markedly inhibited high glucose-induced hepatic lipid accumulation in HepG2 cells. In addition, it reduced the protein expression of lipogenic factors, including sterol regulatory element-binding protein 1c (SREBP-1c) and fatty acid synthase (FAS). Nobiletin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Pretreatment with compound C, an AMPK inhibitor, abolished the inhibitory effects of nobiletin on SREBP-1c and FAS expression. These results suggested that nobiletin might attenuate high glucose-induced lipid accumulation in HepG2 hepatocytes via modulation of AMPK signaling pathway. Therefore, nobiletin might be useful for the prevention and treatment of nonalcoholic fatty liver diseases.

scholarly journals Pyrroloquinoline Quinone Alleviates Oxidative Damage Induced by High Glucose in HepG2 Cells

2021 ◽  
Author(s):  
Saad Alkahtani ◽  
Saud Alarifi ◽  
Abdullah A. Alkahtane ◽  
Gadah Albasher ◽  
Mohammed AL-Zharani ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
High Glucose ◽  
Hepg2 Cells ◽  
Pyrroloquinoline Quinone

Ellipticine induces apoptosis through p53-dependent pathway in human hepatocellular carcinoma HepG2 cells

Life Sciences ◽  
2006 ◽  
Vol 78 (22) ◽  
pp. 2550-2557 ◽  
Author(s):  
Yu-Chun Kuo ◽  
Po-Lin Kuo ◽  
Ya-Ling Hsu ◽  
Chien-Yu Cho ◽  
Chun-Ching Lin
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Human Hepatocellular Carcinoma ◽  
Dependent Pathway

Unsaponifiable Matter from Rice Bran Attenuates High Glucose-Induced Lipid Accumulation by Activating AMPK in HepG2 Cells

2016 ◽  
Vol 41 (2) ◽  
pp. e12313
Author(s):  
Hyeonmi Ham ◽  
Koan Sik Woo ◽  
Yu Young Lee ◽  
Byongwon Lee ◽  
In-Hwan Kim ◽  
...  
Keyword(s):  
Unsaponifiable Matter ◽  
Rice Bran ◽  
High Glucose ◽  
Lipid Accumulation ◽  
Hepg2 Cells

Morin Exerts Anti-Diabetic Effects in Human HepG2 Cells Via Down-Regulation of miR-29a

2018 ◽  
Vol 127 (09) ◽  
pp. 615-622 ◽  
Author(s):  
Toktam Razavi ◽  
Shideh Montasser Kouhsari ◽  
Khalil Abnous
Keyword(s):  
Insulin Signaling ◽  
High Glucose ◽  
Hepg2 Cells ◽  
Target Genes ◽  
Expression Level ◽  
Inhibitory Effects ◽  
Down Regulation ◽  
Insulin Mimetic ◽  
Important Regulator ◽  
First Time

Abstract Diabetes mellitus is a complex metabolic disease around the world that is characterized by hyperglycemia resulting from impaired insulin secretion, insulin action, or both. MicroRNA-29a is an important regulator of insulin signaling and gluconeogenesis pathways through IRS2, PI3K and PEPCK expressions which up regulates in Diabetes. Morin is a substantial bioflavonoid which has insulin mimetic effect, and interacting with nucleic acids and proteins. In this study HepG2 cells, were exposed to high glucose to induce diabetic condition. We have determined whether high glucose stimulation might promotes miR-29a expression level in HepG2 cells and subsequently evaluated the Morin treatment effects on this state. In HepG2 cells, high glucose increases miR-29a expression level and decreases its target genes, IRS2 and PI3K expression, and increases associated downstream gene in gluconeogenic pathway, PEPCK. Morin treatment down regulates miR-29a expression level and improves insulin signaling and glucose metabolism. To confirm the inhibitory effects of Morin on miR-29a, we have transfected cells with mimic and inhibitor-miR-29a. This study for the first time identifies that Morin improves diabetic condition through down regulation of the miR-29a level, and suggest that this new inhibitor of miR-29a may be a useful biomedicine to treat diabetes.

scholarly journals The effects of naringenin and naringin on the glucose uptake and AMPK phosphorylation in high glucose treated HepG2 cells

2021 ◽  
Vol 22 ◽  
Author(s):  
Lakshi A. Dayarathne ◽  
Sachithra S. Ranaweera ◽  
Premkumar Natraj ◽  
Priyanka Rajan ◽  
Young Jae Lee ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
High Glucose ◽  
Hepg2 Cells ◽  
Ampk Phosphorylation

Development of in vitro model of insulin receptor cleavage induced by high glucose in HepG2 cells

2014 ◽  
Vol 445 (1) ◽  
pp. 236-243 ◽  
Author(s):  
Tomoyuki Yuasa ◽  
Kikuko Amo ◽  
Shuhei Ishikura ◽  
Hisao Nagaya ◽  
Keiji Uchiyama ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
High Glucose ◽  
Hepg2 Cells ◽  
In Vitro Model ◽  
Vitro Model
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