Small Differences in SUC Gene Sequences Impact Saccharomyces cerevisiae Invertase Activity and Specificity toward Fructans with Different Chain Lengths

2021 ◽  
Vol 69 (6) ◽  
pp. 1925-1935
Author(s):  
Jitka Laurent ◽  
Anouk Aerts ◽  
Jonathan Gordon ◽  
Purvi Gupta ◽  
Arnout R. D. Voet ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Invertase Activity ◽  
Gene Sequences ◽  
Chain Lengths

scholarly journals Comparison of the Glycolytic and Alcoholic Fermentation Pathways of Hanseniaspora vineae with Saccharomyces cerevisiae Wine Yeasts

Fermentation ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 78 ◽  
Author(s):  
María José Valera ◽  
Eduardo Boido ◽  
Eduardo Dellacassa ◽  
Francisco Carrau
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcoholic Fermentation ◽  
Invertase Activity ◽  
Wine Industry ◽  
Wine Yeasts ◽  
Spontaneous Fermentation ◽  
Fermentative Capacity ◽  
Definition Of ◽  
Concept Framework ◽  
Fast Evolution

Hanseniaspora species can be isolated from grapes and grape musts, but after the initiation of spontaneous fermentation, they are displaced by Saccharomyces cerevisiae. Hanseniaspora vineae is particularly valuable since this species improves the flavour of wines and has an increased capacity to ferment relative to other apiculate yeasts. Genomic, transcriptomic, and metabolomic studies in H. vineae have enhanced our understanding of its potential utility within the wine industry. Here, we compared gene sequences of 12 glycolytic and fermentation pathway enzymes from five sequenced Hanseniaspora species and S. cerevisiae with the corresponding enzymes encoded within the two sequenced H. vineae genomes. Increased levels of protein similarity were observed for enzymes of H. vineae and S. cerevisiae, relative to the remaining Hanseniaspora species. Key differences between H. vineae and H. uvarum pyruvate kinase enzymes might explain observed differences in fermentative capacity. Further, the presence of eight putative alcohol dehydrogenases, invertase activity, and sulfite tolerance are distinctive characteristics of H. vineae, compared to other Hanseniaspora species. The definition of two clear technological groups within the Hanseniaspora genus is discussed within the slow and fast evolution concept framework previously discovered in these apiculate yeasts.

ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae

Nature ◽  
1983 ◽  
Vol 306 (5944) ◽  
pp. 707-709 ◽  
Author(s):  
D. DeFeo-Jones ◽  
E. M. Scolnick ◽  
R. Koller ◽  
R. Dhar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Related Gene ◽  
Gene Sequences

The residual enzymatic phosphorylation activity of hexokinase II mutants is correlated with glucose repression in Saccharomyces cerevisiae

1989 ◽  
Vol 9 (12) ◽  
pp. 5643-5649
Author(s):  
H Ma ◽  
L M Bloom ◽  
C T Walsh ◽  
D Botstein
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Repression ◽  
Point Mutations ◽  
Inverse Correlation ◽  
Invertase Activity ◽  
Yeast Cells ◽  
Hexokinase Ii ◽  
Mutant Proteins ◽  
Hexokinase Activity

Saccharomyces cerevisiae mutants containing different point mutations in the HXK2 gene were used to study the relationship between phosphorylation by hexokinase II and glucose repression in yeast cells. Mutants showing different levels of hexokinase activity were examined for the degree of glucose repression as indicated by the levels of invertase activity. The levels of hexokinase activity and invertase activity showed a strong inverse correlation, with a few exceptions attributable to very unstable hexokinase II proteins. The in vivo hexokinase II activity was determined by measuring growth rates, using fructose as a carbon source. This in vivo hexokinase II activity was similarly inversely correlated with invertase activity. Several hxk2 alleles were transferred to multicopy plasmids to study the effects of increasing the amounts of mutant proteins. The cells that contained the multicopy plasmids exhibited less invertase and more hexokinase activity, further strengthening the correlation. These results strongly support the hypothesis that the phosphorylation activity of hexokinase II is correlated with glucose repression.

scholarly journals Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge

2006 ◽  
Vol 49 (6) ◽  
pp. 873-880 ◽  
Author(s):  
Leila Larisa Medeiros Marques ◽  
João Batista Buzato ◽  
Maria Antonia Pedrine Colabone Celligoi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Culture ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Immobilized Cells ◽  
Invertase Activity ◽  
Free Cell ◽  
Immobilized Cell ◽  
Luffa Cylindrica ◽  
Ultrasound Application

This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These results showed that ultrasound application in continuous culture could have great potential for application in biotechnological techniques.

scholarly journals Regulation of alpha-factor production in Saccharomyces cerevisiae: a-factor pheromone-induced expression of the MF alpha 1 and STE13 genes.

1989 ◽  
Vol 9 (10) ◽  
pp. 4507-4514 ◽  
Author(s):  
T Achstetter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fusion Gene ◽  
Invertase Activity ◽  
Transcriptional Level ◽  
Alpha Cells ◽  
Mating Pheromone ◽  
Induced Expression ◽  
Alpha Factor ◽  
Suc2 Gene ◽  
Proteolytic Activities

Production of the mating pheromone alpha-factor was examined in Saccharomyces cerevisiae MAT alpha cells that had been exposed to the mating pheromone a-factor. A 2-h treatment with a-factor caused a significant increase in alpha-factor concentration in the medium as demonstrated by a halo assay. MF alpha 1 is one of the two genes coding for a precursor of alpha-factor. A Northern (RNA) analysis of total RNA from a-factor-treated MAT alpha cells revealed a rapid two- to threefold increase in MF alpha 1 transcript levels, reaching maximum within 60 min of exposure to the pheromone. Pheromone induction did not require ongoing protein synthesis. a-Factor-induced MF alpha 1 expression was quantitated by analysis of an MF alpha 1::SUC2 fusion gene whose product was assayed for invertase activity. Expression of the MF alpha 1::SUC2 gene in MAT alpha cells responded to the a-factor signal like the chromosomal version of MF alpha 1. Maturation of the alpha-factor precursor involves three proteolytic activities which are encoded by the KEX1, KEX2, and STE13 genes, respectively. Two of these genes, namely, KEX2 and STE13, were examined for pheromone-induced expression. Only the STE13 gene exhibited pheromone induction at the transcriptional level.

scholarly journals Detection of Invertase Activity Produced by Saccharomyces cerevisiae as Source of Sucrose Degradation in Sugarcane Juice

2019 ◽  
pp. 1-7
Author(s):  
Blei Sika Hortense ◽  
Adje Hoba Kevin ◽  
Angaman Djédoux Maxime ◽  
Amiepo N’cho Patrice
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Food Processing ◽  
Dry Matter ◽  
Saccharum Officinarum ◽  
Invertase Activity ◽  
Sugarcane Juice ◽  
Sugar Production ◽  
Good Source ◽  
Favorable Conditions

The decline in purity of sugarcane juice used for the production of sucrose leads to huge losses in food processing chains. However, this sugar is much consumed by the population. Knowledge of the degradation origin of sucrose in the process of transformation is therefore necessary. It was more precisely a matter of assessing the quality of four sugarcane (Saccharum officinarum) juice of different varieties (R 570, R 590, Co 997 and SP 711406) by determining their physicochemical parameters and by highlighting the presence of Saccharomyces cerevisiae and invertase activity produced. These analyses were conducted in the Agrovalorization laboratory of the UFR Agroforesterie; University Jean Lorougnon Guédé, between November 2018 and February 2019. Thus, all juices are acidic (pH 5.05 ± 0.13) with average dry matter and sugar contents of 16.70 ± 1.04 and 59.03 ± 5.02, respectively, with favorable conditions for yeasts proliferation. In addition, S. cerevisiae was found in three juices (R 590, Co 997 and SP 711406) with a specific invertase activity of 0.42 10-2 IU / mg protein. This activity would be the basis of the degradation of sucrose in crude sugarcane juice. On the other hand, the juice resulting from the variety R 570 contained germ deprived of invertase activity (1.57 10-5 IU / mg of proteins). Its use would be a good source of crude materials for optimizing sugar production.

Regulation of alpha-factor production in Saccharomyces cerevisiae: a-factor pheromone-induced expression of the MF alpha 1 and STE13 genes

1989 ◽  
Vol 9 (10) ◽  
pp. 4507-4514
Author(s):  
T Achstetter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fusion Gene ◽  
Invertase Activity ◽  
Transcriptional Level ◽  
Alpha Cells ◽  
Mating Pheromone ◽  
Induced Expression ◽  
Alpha Factor ◽  
Suc2 Gene ◽  
Proteolytic Activities

Production of the mating pheromone alpha-factor was examined in Saccharomyces cerevisiae MAT alpha cells that had been exposed to the mating pheromone a-factor. A 2-h treatment with a-factor caused a significant increase in alpha-factor concentration in the medium as demonstrated by a halo assay. MF alpha 1 is one of the two genes coding for a precursor of alpha-factor. A Northern (RNA) analysis of total RNA from a-factor-treated MAT alpha cells revealed a rapid two- to threefold increase in MF alpha 1 transcript levels, reaching maximum within 60 min of exposure to the pheromone. Pheromone induction did not require ongoing protein synthesis. a-Factor-induced MF alpha 1 expression was quantitated by analysis of an MF alpha 1::SUC2 fusion gene whose product was assayed for invertase activity. Expression of the MF alpha 1::SUC2 gene in MAT alpha cells responded to the a-factor signal like the chromosomal version of MF alpha 1. Maturation of the alpha-factor precursor involves three proteolytic activities which are encoded by the KEX1, KEX2, and STE13 genes, respectively. Two of these genes, namely, KEX2 and STE13, were examined for pheromone-induced expression. Only the STE13 gene exhibited pheromone induction at the transcriptional level.

scholarly journals The Saccharomyces cerevisiae Weak-Acid-Inducible ABC Transporter Pdr12 Transports Fluorescein and Preservative Anions from the Cytosol by an Energy-Dependent Mechanism

1999 ◽  
Vol 181 (15) ◽  
pp. 4644-4652 ◽  
Author(s):  
Caroline D. Holyoak ◽  
Danielle Bracey ◽  
Peter W. Piper ◽  
Karl Kuchler ◽  
Peter J. Coote
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Abc Transporter ◽  
Sorbic Acid ◽  
Surrounding Medium ◽  
Weak Acid ◽  
Water Soluble ◽  
Aliphatic Chain ◽  
Chain Lengths ◽  
Energy Dependent ◽  
Mutant Cells

ABSTRACT Growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid results in the induction of the ATP-binding cassette (ABC) transporter Pdr12 in the plasma membrane (P. Piper, Y. Mahe, S. Thompson, R. Pandjaitan, C. Holyoak, R. Egner, M. Muhlbauer, P. Coote, and K. Kuchler, EMBO J. 17:4257–4265, 1998). Pdr12 appears to mediate resistance to water-soluble, monocarboxylic acids with chain lengths of from C1 to C7. Exposure to acids with aliphatic chain lengths greater than C7 resulted in no observable sensitivity ofΔpdr12 mutant cells compared to the parent. Parent andΔpdr12 mutant cells were grown in the presence of sorbic acid and subsequently loaded with fluorescein. Upon addition of an energy source in the form of glucose, parent cells immediately effluxed fluorescein from the cytosol into the surrounding medium. In contrast, under the same conditions, cells of the Δpdr12 mutant were unable to efflux any of the dye. When both parent andΔpdr12 mutant cells were grown without sorbic acid and subsequently loaded with fluorescein, upon the addition of glucose no efflux of fluorescein was detected from either strain. Thus, we have shown that Pdr12 catalyzes the energy-dependent extrusion of fluorescein from the cytosol. Lineweaver-Burk analysis revealed that sorbic and benzoic acids competitively inhibited ATP-dependent fluorescein efflux. Thus, these data provide strong evidence that sorbate and benzoate anions compete with fluorescein for a putative monocarboxylate binding site on the Pdr12 transporter.

scholarly journals Effects of the carbon source on the physiology and invertase activity of the yeast Saccharomyces cerevisiae FT858

3 Biotech ◽  
2020 ◽  
Vol 10 (8) ◽  
Author(s):  
Valkirea Matos Nascimento ◽  
Gabriela Totino Ulian Antoniolli ◽  
Rodrigo Simões Ribeiro Leite ◽  
Gustavo Graciano Fonseca
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Invertase Activity ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals The residual enzymatic phosphorylation activity of hexokinase II mutants is correlated with glucose repression in Saccharomyces cerevisiae.

1989 ◽  
Vol 9 (12) ◽  
pp. 5643-5649 ◽  
Author(s):  
H Ma ◽  
L M Bloom ◽  
C T Walsh ◽  
D Botstein
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Repression ◽  
Point Mutations ◽  
Inverse Correlation ◽  
Invertase Activity ◽  
Yeast Cells ◽  
Hexokinase Ii ◽  
Mutant Proteins ◽  
Hexokinase Activity

Saccharomyces cerevisiae mutants containing different point mutations in the HXK2 gene were used to study the relationship between phosphorylation by hexokinase II and glucose repression in yeast cells. Mutants showing different levels of hexokinase activity were examined for the degree of glucose repression as indicated by the levels of invertase activity. The levels of hexokinase activity and invertase activity showed a strong inverse correlation, with a few exceptions attributable to very unstable hexokinase II proteins. The in vivo hexokinase II activity was determined by measuring growth rates, using fructose as a carbon source. This in vivo hexokinase II activity was similarly inversely correlated with invertase activity. Several hxk2 alleles were transferred to multicopy plasmids to study the effects of increasing the amounts of mutant proteins. The cells that contained the multicopy plasmids exhibited less invertase and more hexokinase activity, further strengthening the correlation. These results strongly support the hypothesis that the phosphorylation activity of hexokinase II is correlated with glucose repression.

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