scholarly journals Real-Time XANES Measurement of Se Reduction by Zerovalent Iron in a Flow-through Cell, and Accompanying Se Isotope Measurements

2018 ◽  
Vol 52 (16) ◽  
pp. 9304-9310 ◽  
Author(s):  
Heather K. Shrimpton ◽  
Julia H. Jamieson-Hanes ◽  
Carol J. Ptacek ◽  
David W. Blowes
Keyword(s):  
Real Time ◽  
Zerovalent Iron ◽  
Flow Through ◽  
Isotope Measurements

Perchlorate Reduction by Autotrophic Bacteria Attached to Zerovalent Iron in a Flow-Through Reactor

2007 ◽  
Vol 41 (3) ◽  
pp. 990-997 ◽  
Author(s):  
Xueyuan Yu ◽  
Christopher Amrhein ◽  
Marc A. Deshusses ◽  
Mark R. Matsumoto
Keyword(s):  
Zerovalent Iron ◽  
Perchlorate Reduction ◽  
Autotrophic Bacteria ◽  
Flow Through

scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Con A ◽  
Quartz Crystals ◽  
Ulex Europaeus ◽  
Novel Approach ◽  
Ulex Europaeus Agglutinin I ◽  
Flow Through ◽  
Time Determination ◽  
Kinetics Of

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

scholarly journals Real-time optical sensing of exhaled acetone concentration utilizing non-Fickian Nafion diffusion inside a flow-through sample chamber

2020 ◽  
Vol 29 ◽  
pp. 100373 ◽  
Author(s):  
Ulzii-Orshikh Badmaarag ◽  
Jonathan A. Bernstein ◽  
Reza Shekarriz ◽  
Anastasios P. Angelopoulos
Keyword(s):  
Real Time ◽  
Optical Sensing ◽  
Acetone Concentration ◽  
Sample Chamber ◽  
Flow Through

Detection of Salmonella by Flow-Through Immunocapture Real-Time PCR in Selected Foods within 8 Hours

2007 ◽  
Vol 70 (4) ◽  
pp. 1002-1006 ◽  
Author(s):  
BENJAMIN R. WARREN ◽  
HYUN-GYUN YUK ◽  
KEITH R. SCHNEIDER
Keyword(s):  
Real Time ◽  
Drug Administration ◽  
Real Time Pcr ◽  
Food And Drug Administration ◽  
Ground Beef ◽  
Culture Method ◽  
Food Samples ◽  
Flow Through ◽  
Pcr Method

This study investigated flow-through immunocapture (FTI), using the Pathatrix device, followed by plating on xylose lysine desoxycholate (XLD) agar (FTI-XLD) or analysis by real-time PCR (FTI-PCR) for the detection of Salmonella on smooth tomato surfaces and in potato salad and ground beef within 8 h. Food samples were inoculated with an appropriate dilution of a five-serovar Salmonella cocktail and enriched for 5 h. Following enrichment, samples were analyzed by the FTIXLD and FTI-PCR methods. Food samples were also analyzed by a modified U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM) Salmonella culture method for comparison. Salmonella inoculated at 100 CFU per tomato or 100 CFU/25 g was detected by the FTI-XLD method in 6, 8, and 4 of 10 samples for tomatoes, potato salad, and ground beef, respectively. Salmonella inoculated at 100 CFU per tomato or 100 CFU/25 g was detected by the FTI-PCR method in 8, 9, and 9 of 10 samples for tomatoes, potato salad, and ground beef, respectively. The FTI-PCR method achieved significantly higher (P < 0.05) detection of Salmonella on tomatoes, whereas the FTI-XLD method achieved significantly lower (P < 0.05) detection of Salmonella in ground beef when compared with the modified BAM Salmonella culture method; however, all other comparisons to the modified BAM method were not significantly different. The FTI-XLD method demonstrated the ability to isolate presumptive Salmonella colonies up to 48 h faster than did the modified BAM Salmonella culture method.

Hacking Together Globally

2017 ◽  
Vol 3 (1) ◽  
pp. 95-108 ◽  
Author(s):  
Jeremy Hunsinger
Keyword(s):  
Real Time ◽  
Video Analysis ◽  
Physical Space ◽  
Creative Activity ◽  
Video Streams ◽  
The People ◽  
Flow Through ◽  
Global Movements ◽  
Digital Spaces

Abstract This paper examines events that occur synchronously around the globe at hackerspaces: during Global Synchronous Hackathons, participants use video streams to share experiences, work and interact in real time. This paper analyses synchronous hackathons through video repositories of these events. It aims at discerning what norms are enacted in presented hacking experiences and how those norms are communicated across the video streams. Hacking in these cases should be thought of as the creative activity of using technology to build something that solves a problem or challenge. Hackerspaces are social workshops and communities renting a physical space and usually interacting in digital spaces. In these environments, individuals are involved in hacking as combined social as well as solitary activities which, to some extent, embody certain norms. Individuals also create the “technological drama”; that is they create the discourse around the objects that inform their use and embed them in cultures. These cultures and their discourses possess norms which flow through them and exist around the objects. Members of hackerspaces commonly participate in the aforementioned “Synchronous Hackathons.” By comparing videos of these hackathons, I stress the relevance of norms which are not usually listed in reflections on hacker ethics such as those of Steven Levy or Pekka Himmanen: the awareness of the global other or the awareness of what might be termed “the cosmopolitical.” These norms seek to care for and attend to the people who exist at a distance. This transformation of local to global “hacker ethics” demonstrates the growth of the recognition, at least internally, that hackerspaces embody more than their local concerns: they are part of global movements with global interests and globalising norms. The video analysis is used to demonstrate the globalising norms of these communities as the norms surrounding cosmopolitics become more prevalent in their discourses.

scholarly journals Flow-Through Porous Silicon Membranes for Real-Time Label-Free Biosensing

2016 ◽  
Vol 88 (22) ◽  
pp. 10940-10948 ◽  
Author(s):  
Yiliang Zhao ◽  
Girija Gaur ◽  
Scott T. Retterer ◽  
Paul E. Laibinis ◽  
Sharon M. Weiss
Keyword(s):  
Porous Silicon ◽  
Real Time ◽  
Label Free ◽  
Flow Through ◽  
Silicon Membranes

Improved Chamber Systems for Rapid, Real-Time Nitrous Oxide Emissions from Manure and Soil

2017 ◽  
Vol 60 (4) ◽  
pp. 1235-1258 ◽  
Author(s):  
David B. Parker ◽  
Kenneth D. Casey ◽  
Richard W. Todd ◽  
Heidi M. Waldrip ◽  
Gary M. Marek ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Greenhouse Gas ◽  
Real Time ◽  
Nitrous Oxide Emissions ◽  
Chamber System ◽  
Flux Chamber ◽  
Total N ◽  
Recirculating Flow ◽  
Flux Measurements ◽  
Flow Through

Abstract. Nitrous oxide (N2O) emission rates have traditionally been measured using non-flow-through (NFT), non-steady-state (NSS) chambers, which rely on measuring the increase in N2O concentration in the sealed chamber headspace over time. These flux measurements are very labor- and time-intensive, requiring three to four gas samples collected over a 30 to 60 min period, followed by laboratory N2O measurement with a gas chromatograph (GC) and subsequent flux rate calculation. The objective of this research was to develop and evaluate improved, real-time flux chamber designs that rapidly quantify N2O emissions from manure and soil. The first chamber system consisted of six square 0.95 m2 chamber pans. The chamber pans were mounted on a rail system to facilitate controlled indoor/outdoor laboratory research at a pilot scale. An aluminum lid was moved among the chamber pans. A second portable chamber system with a circular footprint (0.49 m internal dia.) was designed for use in field measurements. With both systems, N2O concentrations were measured each second with 0.1 ppb resolution by recirculating sample air through a real-time continuous N2O analyzer with return flow into the recirculating-flow-through (RFT-NSS) chamber. Performance and observational data are presented for different chamber vent designs, sealing mechanisms between the chamber pan and lid, recirculation pumps, and presence/absence of an internal fan that mixes headspace air within the sealed chamber. As examples of the repeatability and precision of the methodology, ten consecutive flux measurements were obtained using moist manure (32.6% wet basis water content, WCWB) within a 15 min period in which chamber pans were fitted with lids for 60 s and removed for 30 s. The mean calculated N2O flux was 43.08 ±0.89 mg N2O m-2 h-1. Using dry manure (WCWB = 10.8%), five consecutive flux measurements showed a very low, but consistent, flux that averaged 0.025 ±0.0016 mg N2O m-2 h-1. Five case study experiments demonstrate the usefulness of these chamber systems and highlight discoveries and lessons learned to enhance future research efforts. Major discoveries and observations include: (1) installation of a small internal fan within the chamber lids decreased N2O fluctuation over small time periods, allowing precise measurement of manure N2O fluxes as low as 0.0073 mg N2O m-2 h-1 during a 60 s measurement period; (2) two distinct N2O peaks were observed at 1 and 21 d following the addition of water to manure (initial WCWB = 32.6%), with the second peak accounting for 83% of the total N2O emitted over 45 d; and (3) there was notable diurnal variation in N2O fluxes due to temperature variation, even when the manure was dry (WCWB = 10.8%). These flux chamber systems proved to be more rapid, precise, and repeatable than traditional flux chamber methods and offer promise for future greenhouse gas emissions research on manure and soil. Keywords: Cattle, Chamber, Diurnal, Fan, Feedlot, Greenhouse gas, Manure, Precision.

sbRIO Lab–On–Chip Gas Sensing and Monitoring for Multisensory Array

2013 ◽  
Vol 61 (2) ◽  
Author(s):  
Siti Noradhlia Mohamad Tukijan ◽  
Mohd Azhar Abdul Razak ◽  
Fauzan Khairi Che Harun
Keyword(s):  
Response Time ◽  
Real Time ◽  
Virtual Instrument ◽  
Room Temperature ◽  
Gas Sensing ◽  
Fast Response ◽  
Lab On Chip ◽  
Flow Through ◽  
On Chip ◽  
Early Preparation

It is utmost to create a system at which can monitor and indicate the gas level exist in certain area, especially for hazardous gas, as early preparation and protection before something worst happen. The gas sensing and monitoring system composes of hardware and software elements. A spiral chamber which is simplified on chip, known as Lab–On–Chip (LOC), plays an important role in this system. The examined gases will be analysed by 16 sensors during the flow through the spiral chamber. The responses of these sensors are obtained via analogue input channels from single board RIO (sbRIO) and are displayed on a computer using LabVIEW virtual instrument software. The system offers portable, real–time monitoring and fast response time even in room temperature.

Sign in / Sign up

Export Citation Format

Share Document