Characterization of Mechanisms of Glutathione Conjugation with Halobenzoquinones in Solution and HepG2 Cells

2018 ◽  
Vol 52 (5) ◽  
pp. 2898-2908 ◽  
Author(s):  
Wei Wang ◽  
Yichao Qian ◽  
Jinhua Li ◽  
Naif Aljuhani ◽  
Arno G. Siraki ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Glutathione Conjugation

scholarly journals Synthesis, Characterization of Liposomes Modified with Biosurfactant MEL-A Loading Betulinic Acid and Its Anticancer Effect in HepG2 Cell

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3939 ◽  
Author(s):  
Qin Shu ◽  
Jianan Wu ◽  
Qihe Chen
Keyword(s):  
Betulinic Acid ◽  
Hepg2 Cells ◽  
Lipid A ◽  
Clinical Applications ◽  
Dose Effect ◽  
Anticancer Effect ◽  
Mannosylerythritol Lipid ◽  
Anti Cancer ◽  
Effect Relation

As a novel natural compound delivery system, liposomes are capable of incorporating lipophilic bioactive compounds with enhanced compound solubility, stability and bioavailability, and have been successfully translated into real-time clinical applications. To construct the soy phosphatidylcholine (SPC)–cholesterol (Chol) liposome system, the optimal formulation was investigated as 3:1 of SPC to Chol, 10% mannosylerythritol lipid-A (MEL-A) and 1% betulinic acid. Results show that liposomes with or without betulinic acid or MEL-A are able to inhibit the proliferation of HepG2 cells with a dose-effect relation remarkably. In addition, the modification of MEL-A in liposomes can significantly promote cell apoptosis and strengthen the destruction of mitochondrial membrane potential in HepG2 cells. Liposomes containing MEL-A and betulinic acid have exhibited excellent anticancer activity, which provide factual basis for the development of MEL-A in the anti-cancer applications. These results provide a design thought to develop delivery liposome systems carrying betulinic acid with enhanced functional and pharmaceutical attributes.

scholarly journals Characterization of hepcidin response to holotransferrin in novel recombinant TfR1 HepG2 cells

2016 ◽  
Vol 61 ◽  
pp. 37-45 ◽  
Author(s):  
Kosha Mehta ◽  
Mark Busbridge ◽  
Derek Renshaw ◽  
Robert W. Evans ◽  
Sebastien Farnaud ◽  
...  
Keyword(s):  
Hepg2 Cells

scholarly journals Characterization of Purified Red Cabbage Anthocyanins: Improvement in HPLC Separation and Protective Effect against H2O2-Induced Oxidative Stress in HepG2 Cells

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 124 ◽  
Author(s):  
Sheng Fang ◽  
Fubin Lin ◽  
Daofeng Qu ◽  
Xianrui Liang ◽  
Liping Wang
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Hplc Separation ◽  
Column Temperature ◽  
Red Cabbage ◽  
Chemical Profiles ◽  
Derivatives Of ◽  
Intracellular Oxidative Stress

In this study, the chemical profiles and antioxidant activities of red cabbage anthocyanin (RCA)-enriched extract are evaluated. The effects of column temperature on the HPLC resolution of the RCAs are studied. The HPLC resolutions became better as the column temperature increased from 20 °C–45 °C. An optimized HPLC condition was achieved at 45 °C and used for the quantification and qualification of the RCAs. The anthocyanins in the enriched powder are all derivatives of cyanidin (268 ± 2 μg/mg), mainly with 19% nonacylated, 51% monoacylated, and 31% diacylated structures with ferulic, sinapic, p-coumaric, and caffeic acids characterized by HPLC-MS. The RCA extracts markedly reduced intracellular oxidative stress production by H2O2 on HepG2 cells and consequently ameliorated cell apoptosis and improved viability. The analytical method and cellular antioxidant activity demonstration of the RCAs will greatly facilitate their functional applications.

scholarly journals Characterization of the contribution of spliced RNAs of hepatitis B virus to DNA synthesis in transfected cultures of Huh7 and HepG2 cells

Virology ◽  
2008 ◽  
Vol 379 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Teresa M. Abraham ◽  
Eric B. Lewellyn ◽  
Kathleen M. Haines ◽  
Daniel D. Loeb
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Synthesis ◽  
Hepg2 Cells ◽  
B Virus
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