scholarly journals Mechanisms of Action of Compounds That Enhance Storage Lipid Accumulation in Daphnia magna

2016 ◽  
Vol 50 (24) ◽  
pp. 13565-13573 ◽  
Author(s):  
Rita Jordão ◽  
Bruno Campos ◽  
Benjamín Piña ◽  
Romà Tauler ◽  
Amadeu M. V. M. Soares ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Lipid Accumulation ◽  
Mechanisms Of Action ◽  
Storage Lipid

Storage lipid accumulation and acyltransferase action in developing flaxseed

Lipids ◽  
2005 ◽  
Vol 40 (10) ◽  
pp. 1043-1049 ◽  
Author(s):  
Brent M. Sørensen ◽  
Tara L. Furukawa-Stoffer ◽  
Kris S. Marshall ◽  
Erin K. Page ◽  
Zahir Mir ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Storage Lipid

scholarly journals Mono(2-ethylhexyl) Phthalate Modulates the Lipid Accumulation and Reproductive Signaling in Daphnia Magna

2021 ◽  
Author(s):  
Hyunki Cho ◽  
Yohan Seol ◽  
Seungyun Baik ◽  
Baeckkyoung Sung ◽  
Chang Seon Ryu ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Exposure Time ◽  
Lipid Accumulation ◽  
Aquatic Organisms ◽  
Treated Group ◽  
Ecdysone Receptor ◽  
Female Adult ◽  
Primary Metabolite ◽  
Lipid Metabolites ◽  
Ethylhexyl Phthalate

Abstract Mono(2-ethylhexyl)-phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl)-phthalate (DEHP), which is widely used in industry as a plasticizer. Previous studies showed that both DEHP and MEHP have been found in not only human urine samples but also natural aquatic environments, and well documented as toxicants for reproduction and endocrine disruptors, However, the effects of MEHP exposure on aquatic organisms, including invertebrates such as Daphnia magna (D. magna), are still scarce. In the present study, the lipid alterations caused by MEHP in D. magna have been identified by analyzing lipid accumulation and nontarget metabolomics. Additionally, the expressions of reproduction were investigated. Ecotoxicologically relevant concentrations (1 to 2 mg/L) and exposure time ranges (24 h to 21 days) have been tested. MEHP resulted in no mortality to D. magna with all exposure conditions, but the number of lipid droplets increased after 96 h of exposure and enhanced reproduction of female adult daphnids was observed in the 21-day of exposure. Besides, we observed that MEHP enhanced lipid metabolism with the identification of 283 potential lipid metabolites, including glycerolipids, glycerophospholipids, and sphingolipids, following 48 h of exposure. MEHP treated group exhibited significantly higher expression of ecdysone receptor (EcR) and vitellogenin 2 (Vtg2) at 6 h and 24 h. Meanwhile at 48 h, EcR and Vtg2 were downregulated in 1 and 2 mg/L MEHP exposure. Our data show that the changes in EcR pathway with different exposure time could be associated with the lipid accumulation due to lipids increment subsequently to increased reproduction in MEHP-exposed D. magna.

scholarly journals Storage lipid accumulation is controlled by photoperiodic signal acting via regulators of growth cessation and dormancy in hybrid aspen

2018 ◽  
Vol 219 (2) ◽  
pp. 619-630 ◽  
Author(s):  
Åsa Grimberg ◽  
Ida Lager ◽  
Nathaniel R. Street ◽  
Kathryn M. Robinson ◽  
Salla Marttila ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Hybrid Aspen ◽  
Growth Cessation ◽  
Storage Lipid

Abiotic factors influence plant storage lipid accumulation and composition

Plant Science ◽  
2016 ◽  
Vol 243 ◽  
pp. 1-9 ◽  
Author(s):  
Stacy D. Singer ◽  
Jitao Zou ◽  
Randall J. Weselake
Keyword(s):  
Lipid Accumulation ◽  
Abiotic Factors ◽  
Storage Lipid ◽  
Plant Storage

scholarly journals Analysis of Storage Lipid Accumulation in Alcanivorax borkumensis: Evidence for Alternative Triacylglycerol Biosynthesis Routes in Bacteria

2006 ◽  
Vol 189 (3) ◽  
pp. 918-928 ◽  
Author(s):  
Rainer Kalscheuer ◽  
Tim Stöveken ◽  
Ursula Malkus ◽  
Rudolf Reichelt ◽  
Peter N. Golyshin ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Bacterial Species ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Wax Ester ◽  
Hydrocarbonoclastic Bacteria ◽  
Storage Lipids ◽  
Marine Habitats ◽  
Storage Lipid ◽  
Tag Biosynthesis

ABSTRACT Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor challenging those bacteria in their natural marine habitats. Here we report on the analysis of storage lipid biosynthesis in A. borkumensis strain SK2. Triacylglycerols (TAGs) and wax esters (WEs), but not poly(hydroxyalkanoic acids), are the principal storage lipids present in this and other hydrocarbonoclastic bacterial species. Although so far assumed to be a characteristic restricted to gram-positive actinomycetes, substantial accumulation of TAGs corresponding to a fatty acid content of more than 23% of the cellular dry weight is the first characteristic of large-scale de novo TAG biosynthesis in a gram-negative bacterium. The acyltransferase AtfA1 (ABO_2742) exhibiting wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) activity plays a key role in both TAG and WE biosynthesis, whereas AtfA2 (ABO_1804) was dispensable for storage lipid formation. However, reduced but still substantial residual TAG levels in atfA1 and atfA2 knockout mutants compellingly indicate the existence of a yet unknown WS/DGAT-independent alternative TAG biosynthesis route. Storage lipids of A. borkumensis were enriched in saturated fatty acids and accumulated as insoluble intracytoplasmic inclusions exhibiting great structural variety. Storage lipid accumulation provided only a slight growth advantage during short-term starvation periods but was not required for maintaining viability and long-term persistence during extended starvation phases.

Membrane-bound vesicles associated with the microvilli in the midgut of the freshwater microcrustacean, Daphnia magna

1983 ◽  
Vol 41 ◽  
pp. 480-481
Author(s):  
Patricia L. Jansma
Keyword(s):  
Daphnia Magna ◽  
Intestinal Epithelial Cells ◽  
Uranyl Acetate ◽  
Intestinal Epithelial ◽  
Chlamydomonas Reinhardii ◽  
Thin Sections ◽  
Saturated Water ◽  
Cacodylate Buffer ◽  
Membrane Bound ◽  
Ethanol Series

The presence of the membrane bound vesicles or blebs on the intestinal epithelial cells has been demonstrated in a variety of vertebrates such as chicks, piglets, hamsters, and humans. The only invertebrates shown to have these microvillar blebs are two species of f1ies. While investigating the digestive processes of the freshwater microcrustacean, Daphnia magna, the presence of these microvillar blebs was noticed.Daphnia magna fed in a suspension of axenically grown green alga, Chlamydomonas reinhardii for one hour were narcotized with CO2 saturated water. The intestinal tracts were excised in 2% glutaraldehyde in 0.2 M cacodyl ate buffer and then placed in fresh 2% glutaraldehyde for one hour. After rinsing in 0.1 M cacodylate buffer, the sample was postfixed in 2% OsO4, dehydrated with a graded ethanol series, infiltrated and embedded with Epon-Araldite. Thin sections were stained with uranyl acetate and Reynolds lead citrate before viewing with the Philips EM 200.

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