scholarly journals Nitrate Reverses Severe Nitrite Inhibition of Anaerobic Ammonium Oxidation (Anammox) Activity in Continuously-Fed Bioreactors

2016 ◽  
Vol 50 (19) ◽  
pp. 10518-10526 ◽  
Author(s):  
Guangbin Li ◽  
Reyes Sierra-Alvarez ◽  
David Vilcherrez ◽  
Stefan Weiss ◽  
Callie Gill ◽  
...  
Keyword(s):  
Anaerobic Ammonium Oxidation ◽  
Ammonium Oxidation ◽  
Anammox Activity ◽  
Nitrite Inhibition

scholarly journals Correction to Nitrate Reverses Severe Nitrite Inhibition of Anaerobic Ammonium Oxidation (Anammox) Activity in Continuously-Fed Bioreactors

2016 ◽  
Vol 50 (22) ◽  
pp. 12526-12526
Author(s):  
Guangbin Li ◽  
Reyes Sierra-Alvarez ◽  
David Vilcherrez ◽  
Stefan Weiss ◽  
Callie Gill ◽  
...  
Keyword(s):  
Anaerobic Ammonium Oxidation ◽  
Ammonium Oxidation ◽  
Anammox Activity ◽  
Nitrite Inhibition

Anaerobic ammonium oxidation of ammonium-rich waste streams in fixed-bed reactors

2004 ◽  
Vol 49 (11-12) ◽  
pp. 77-82 ◽  
Author(s):  
C. Fux ◽  
V. Marchesi ◽  
I. Brunner ◽  
H. Siegrist
Keyword(s):  
Removal Rate ◽  
Elimination Rate ◽  
Fixed Bed ◽  
Treatment Plant ◽  
Experimental Period ◽  
Anaerobic Ammonium Oxidation ◽  
Synthetic Wastewater ◽  
Ammonium Oxidation ◽  
Fixed Bed Reactors ◽  
Anammox Activity

The feasibility of anaerobic ammonium oxidation (Anammox) in fixed-bed reactors was evaluated on laboratory and pilot scales. Using synthetic wastewater, the specific nitrogen removal rate was increased from 0.05-0.1 kgNm-3reactord-1 to 0.35-0.38 kgNm-3reactord-1 within a year (T = 22-27°C) in all applications. However, the anammox activity was seriously and repeatedly inhibited at prolonged high nitrite concentrations (e.g. six days at 30-50 gNO2-Nm-3) and recovery was always a lengthy process. But even at a moderate nitrite concentration (11 ± 10 gNO2-Nm-3), the observed specific growth rate was only 0.018 d-1 at 26.4 ± 0.8°C, which corresponds to approximately 0.025 d-1 at 30°C (doubling time: 28 days). In a second experimental period for another 250 days, one of the laboratory reactors was fed with partially nitrified sludge liquors from a domestic wastewater treatment plant (WWTP). In this case, the specific elimination rate was as high as 3.5 kgNm-3reactord-1 at 26-27°C. Independently of the feed, the average nitrogen elimination rate lay between 80-85% in all applications. An appropriate hydraulic design is essential to prevent clogging and local nitrite inhibition in fixed-bed reactors.

Effects of nitrite inhibition on anaerobic ammonium oxidation

2009 ◽  
Vol 86 (1) ◽  
pp. 359-365 ◽  
Author(s):  
Yuya Kimura ◽  
Kazuichi Isaka ◽  
Futaba Kazama ◽  
Tatsuo Sumino
Keyword(s):  
Anaerobic Ammonium Oxidation ◽  
Ammonium Oxidation ◽  
Nitrite Inhibition

Enrichment of Anaerobic Ammonium Oxidation (Anammox) Bacteria for Biological Nitrogen Removal of Wastewater

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Norjan Yusof ◽  
Hanisom Abdullah ◽  
Syakirah Samsudin ◽  
Mohd Ali Hassan
Keyword(s):  
Batch Reactor ◽  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Leachate Treatment ◽  
16S Rrna Sequence ◽  
Bottom Part ◽  
Enrichment Cultures ◽  
Anammox Activity ◽  
Biological Nitrogen

Anaerobic ammonium oxidation (anammox) bacteria enrichment was explored for the potential application of ammonium rich wastewater removal. Samples of sludge from mature and young landfill leachate treatment plants were screened and used as inocula for anammox enrichment cultures. Enrichments were monitored for N-NH3, N-NO2- and N-NO3- to detect anammox potential activity. Six of the twelve enrichment cultures showed anammox activity after more than five months of enrichment period. All enrichment cultures that gave positive results were obtained from bottom part of sequencing batch reactor (SBR) lagoon indicating localization of anammox bacteria in anaerobic condition.  Polymerase Chain Reaction (PCR) with specific primers targeting anammox and planctomycete were able to amplify the 16S rRNA sequence for anammox bacteria under PCR optimum condition. However, only three of six positive samples were successfully sequenced. DNA sequence analysis using NCBI (BLAST) and RDP showed that the anammox bacterial sequences of the investigated samples were identified as Candidatus Kuenenia stuttgartiensis with similarity of 100% (NCBI) and 99.3% (RDP).

Kinetics and thermodynamics of anaerobic ammonium oxidation process using Brocadia spp. dominated mixed cultures

2014 ◽  
Vol 69 (8) ◽  
pp. 1682-1688 ◽  
Author(s):  
D. Puyol ◽  
J. M. Carvajal-Arroyo ◽  
B. Garcia ◽  
R. Sierra-Alvarez ◽  
J. A. Field
Keyword(s):  
Growth Parameters ◽  
Correlation Coefficients ◽  
Granular Sludge ◽  
Mixed Cultures ◽  
Anaerobic Ammonium Oxidation ◽  
Energy Of Activation ◽  
Organic Carbon Content ◽  
Ammonium Oxidation ◽  
Anammox Activity ◽  
Content Modeling

Anaerobic ammonium oxidation (anammox) is a recently discovered microbial process commonly applied to treat ammonium pollution in effluents with low organic carbon content. Modeling anammox processes is important for simulating and controlling full-scale plants. In this study, the anammox process was simulated using three models, and substrate and growth parameters obtained by different research groups. Two Brocadia spp.-dominated mixed cultures, one granular and the other flocculent, were used for this purpose. A very good correlation between experimental data using both sludges and model predictions was achieved by one of the models, obtaining correlation coefficients higher than 0.997. Other models and stoichiometric equations tested were unable to predict the anammox kinetics and stoichiometry. Furthermore, the thermodynamic behavior of the two mixed cultures was compared through the determination of the energy of activation of the anammox conversion at temperatures ranging from 9 to 40 °C. Optimum temperature for anammox activity was established at 30–35 °C in both cases. The energy of activation values calculated for granular sludge and flocculent sludge were 64 and 124 kJ mol−1, respectively.

Synergistic inhibition of anaerobic ammonium oxidation (anammox) activity by phenol and thiocyanate

Chemosphere ◽  
2018 ◽  
Vol 213 ◽  
pp. 498-506 ◽  
Author(s):  
Mamoru Oshiki ◽  
Yoshiko Masuda ◽  
Takashi Yamaguchi ◽  
Nobuo Araki
Keyword(s):  
Anaerobic Ammonium Oxidation ◽  
Ammonium Oxidation ◽  
Synergistic Inhibition ◽  
Anammox Activity

scholarly journals Nitrate-Dependent Ferrous Iron Oxidation by Anaerobic Ammonium Oxidation (Anammox) Bacteria

2013 ◽  
Vol 79 (13) ◽  
pp. 4087-4093 ◽  
Author(s):  
M. Oshiki ◽  
S. Ishii ◽  
K. Yoshida ◽  
N. Fujii ◽  
M. Ishiguro ◽  
...  
Keyword(s):  
Iron Oxidation ◽  
Reduction Rate ◽  
Tracer Experiment ◽  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Nitrogen Gas ◽  
Content Type ◽  
Ferrous Iron Oxidation ◽  
Anammox Activity

ABSTRACTWe examined nitrate-dependent Fe2+oxidation mediated by anaerobic ammonium oxidation (anammox) bacteria. Enrichment cultures of “CandidatusBrocadia sinica” anaerobically oxidized Fe2+and reduced NO3−to nitrogen gas at rates of 3.7 ± 0.2 and 1.3 ± 0.1 (mean ± standard deviation [SD]) nmol mg protein−1min−1, respectively (37°C and pH 7.3). This nitrate reduction rate is an order of magnitude lower than the anammox activity of “Ca. Brocadia sinica” (10 to 75 nmol NH4+mg protein−1min−1). A15N tracer experiment demonstrated that coupling of nitrate-dependent Fe2+oxidation and the anammox reaction was responsible for producing nitrogen gas from NO3−by “Ca. Brocadia sinica.” The activities of nitrate-dependent Fe2+oxidation were dependent on temperature and pH, and the highest activities were seen at temperatures of 30 to 45°C and pHs ranging from 5.9 to 9.8. The mean half-saturation constant for NO3−± SD of “Ca. Brocadia sinica” was determined to be 51 ± 21 μM. Nitrate-dependent Fe2+oxidation was further demonstrated by another anammox bacterium, “CandidatusScalindua sp.,” whose rates of Fe2+oxidation and NO3−reduction were 4.7 ± 0.59 and 1.45 ± 0.05 nmol mg protein−1min−1, respectively (20°C and pH 7.3). Co-occurrence of nitrate-dependent Fe2+oxidation and the anammox reaction decreased the molar ratios of consumed NO2−to consumed NH4+(ΔNO2−/ΔNH4+) and produced NO3−to consumed NH4+(ΔNO3−/ΔNH4+). These reactions are preferable to the application of anammox processes for wastewater treatment.

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