scholarly journals Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213

Biochemistry ◽  
2019 ◽  
Vol 58 (10) ◽  
pp. 1411-1422 ◽  
Author(s):  
Ilia G. Denisov ◽  
Yelena V. Grinkova ◽  
Prithviraj Nandigrami ◽  
Mrinal Shekhar ◽  
Emad Tajkhorshid ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Allosteric Interactions ◽  
Human Cytochrome

scholarly journals Correction to “Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213”

Biochemistry ◽  
2019 ◽  
Vol 58 (24) ◽  
pp. 2796-2796
Author(s):  
Ilia G. Denisov ◽  
Yelena V. Grinkova ◽  
Prithviraj Nandigrami ◽  
Mrinal Shekhar ◽  
Emad Tajkhorshid ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Allosteric Interactions ◽  
Human Cytochrome

scholarly journals Pathways of Carbamazepine Bioactivation in Vitro. III. The Role of Human Cytochrome P450 Enzymes in the Formation of 2,3-Dihydroxycarbamazepine

2008 ◽  
Vol 36 (8) ◽  
pp. 1637-1649 ◽  
Author(s):  
Robin E. Pearce ◽  
Wei Lu ◽  
YongQiang Wang ◽  
Jack P. Uetrecht ◽  
Maria Almira Correia ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

Role of individual human cytochrome P450 enzymes in thein vitrometabolism of hydromorphone

Xenobiotica ◽  
2004 ◽  
Vol 34 (4) ◽  
pp. 335-344 ◽  
Author(s):  
S. A. Benetton ◽  
V. M. Borges ◽  
T. K. H. Chang ◽  
K. M. Mcerlane
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome ◽  
Individual Human

The role of human cytochrome P450 enzymes in metabolism of acrylamidein vitro

2013 ◽  
Vol 23 (5) ◽  
pp. 346-351 ◽  
Author(s):  
Daria Kraus ◽  
Dennis Rokitta ◽  
Uwe Fuhr ◽  
Dorota Tomalik-Scharte
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

scholarly journals Mechanism of 17α,20-Lyase and New Hydroxylation Reactions of Human Cytochrome P450 17A1

2016 ◽  
Vol 291 (33) ◽  
pp. 17143-17164 ◽  
Author(s):  
Francis K. Yoshimoto ◽  
Eric Gonzalez ◽  
Richard J. Auchus ◽  
F. Peter Guengerich
Keyword(s):  
Acetic Acid ◽  
Cytochrome P450 ◽  
Bond Cleavage ◽  
Active Species ◽  
Sterol Biosynthesis ◽  
Compound I ◽  
Human Cytochrome ◽  
Acetyl Radical

Cytochrome P450 (P450) reactions can involve C–C bond cleavage, and several of these are critical in steroid and sterol biosynthesis. The mechanisms of P450s 11A1, 17A1, 19A1, and 51A1 have been controversial, in the context of the role of ferric peroxide (FeO2−) versus perferryl (FeO3+, compound I) chemistry. We reinvestigated the 17α-hydroxyprogesterone and 17α-hydroxypregnenolone 17α,20-lyase reactions of human P450 17A1 and found incorporation of one 18O atom (from 18O2) into acetic acid, consonant with proposals for a ferric peroxide mechanism (Akhtar, M., Lee-Robichaud, P., Akhtar, M. E., and Wright, J. N. (1997) J. Steroid Biochem. Mol. Biol. 61, 127–132; Akhtar, M., Wright, J. N., and Lee-Robichaud, P. (2011) J. Steroid Biochem. Mol. Biol. 125, 2–12). However, the reactions were supported by iodosylbenzene (a precursor of the FeO3+ species) but not by H2O2. We propose three mechanisms that can involve the FeO3+ entity and that explain the 18O label in the acetic acid, two involving the intermediacy of an acetyl radical and one a steroid 17,20-dioxetane. P450 17A1 was found to perform 16-hydroxylation reactions on its 17α-hydroxylated products to yield 16,17α-dihydroxypregnenolone and progesterone, suggesting the presence of an active perferryloxo active species of P450 17A1 when its lyase substrate is bound. The 6β-hydroxylation of 16α,17α-dihydroxyprogesterone and the oxidation of both 16α,17α-dihydroxyprogesterone and 16α,17α-dihydroxypregnenolone to 16-hydroxy lyase products were also observed. We provide evidence for the contribution of a compound I mechanism, although contribution of a ferric peroxide pathway in the 17α,20-lyase reaction cannot be excluded.

The Role of Ile476 in the Structural Stability and Substrate Binding of Human Cytochrome P450 2C8

2009 ◽  
Vol 29 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Lu Sun ◽  
Zhong-Hua Wang ◽  
Feng-Yun Ni ◽  
Xiang-Shi Tan ◽  
Zhong-Xian Huang
Keyword(s):  
Cytochrome P450 ◽  
Structural Stability ◽  
Substrate Binding ◽  
Cytochrome P450 2C8 ◽  
Human Cytochrome
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