A Multimodal Spectroscopic Imaging Method To Characterize the Metal and Macromolecular Content of Proteinaceous Aggregates (“Amyloid Plaques”)

Biochemistry ◽  
2017 ◽  
Vol 56 (32) ◽  
pp. 4107-4116 ◽  
Author(s):  
Kelly L. Summers ◽  
Nicholas Fimognari ◽  
Ashley Hollings ◽  
Mitchell Kiernan ◽  
Virginie Lam ◽  
...  
Keyword(s):  
Spectroscopic Imaging ◽  
Amyloid Plaques ◽  
Imaging Method ◽  
Macromolecular Content

4797616 High speed NMR spectroscopic imaging method

1990 ◽  
Vol 8 (1) ◽  
pp. V
Author(s):  
Shigeru Matsui ◽  
Takash Onodera ◽  
Kensuke Sekihara ◽  
Hidemi Shiono ◽  
Hideki Kohno
Keyword(s):  
High Speed ◽  
Spectroscopic Imaging ◽  
Imaging Method

A fast spectroscopic imaging method using a blipped phase encode gradient

1989 ◽  
Vol 12 (3) ◽  
pp. 306-315 ◽  
Author(s):  
Peter Webb ◽  
Daniel Spielman ◽  
Albert Macovski
Keyword(s):  
Spectroscopic Imaging ◽  
Imaging Method ◽  
Phase Encode Gradient ◽  
Phase Encode

scholarly journals Tissue Dynamics Spectroscopic Imaging: Functional Imaging of Heterogeneous Cancer Tissue

2020 ◽  
Author(s):  
Zhe Li ◽  
Bihe Hu ◽  
Guang Li ◽  
Sharon E. Fox ◽  
Shadia Jalal ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Tumor Heterogeneity ◽  
Drug Response ◽  
Ex Vivo ◽  
Spectroscopic Imaging ◽  
Cancer Tissue ◽  
Imaging Method ◽  
Spatial Response ◽  
Chemotherapeutic Treatment ◽  
Spatial Maps

AbstractSignificanceTumor heterogeneity poses a challenge for the chemotherapeutic treatment of cancer. Tissue dynamics spectroscopy (TDS) captures dynamic contrast and can capture the response of living tissue to applied therapeutics, but the current analysis averages over the complicated spatial response of living biopsy samples.AimTo develop tissue dynamics spectroscopic imaging (TDSI) to map the heterogeneous spatial response of tumor tissue to anticancer drugs.ApproachTDSI is applied to tumor spheroids grown from cell lines and to ex vivo living esophageal biopsy samples. Doppler fluctuation spectroscopy is performed on a voxel basis to extract spatial maps of biodynamic biomarkers. Functional images and bivariate spatial maps are produced using a bivariate color merge to represent the spatial distribution of pairs of signed drug-response biodynamic biomarkers.ResultsWe have mapped the spatial variability of drug responses within biopsies and have tracked sample-to-sample variability. Sample heterogeneity observed in the biodynamic maps is associated with histological heterogeneity observed using inverted Selective-Plane Illumination Microscopy (iSPIM).ConclusionWe have demonstrated the utility of TDSI as a functional imaging method to measure tumor heterogeneity and its potential for use in drug-response profiling.

Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

1976 ◽  
Vol 34 ◽  
pp. 590-591
Author(s):  
T. Y. Tan ◽  
W. K. Tice
Keyword(s):  
Fast Neutron ◽  
Rapid Determination ◽  
Stacking Faults ◽  
Imaging Method ◽  
Large Reduction ◽  
Dislocation Loops ◽  
Fringe Spacing ◽  
Weak Beam ◽  
Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

Visualisation of E. coli ribosomal RNA in situ by electron spectroscopic imaging and image averaging

1992 ◽  
Vol 50 (1) ◽  
pp. 466-467
Author(s):  
Daniel Beniac ◽  
George Harauz
Keyword(s):  
Ribosomal Rna ◽  
Three Dimensional ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
E Coli ◽  
Microscopical Technique ◽  
Quantitative Image ◽  
Dimensional Reconstruction ◽  
Image Averaging ◽  
Protein Components

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.

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