High-Throughput Manipulation of Circulating Tumor Cells Using a Multiple Single-Cell Encapsulation System with a Digital Micromirror Device

2018 ◽  
Vol 90 (16) ◽  
pp. 9734-9741 ◽  
Author(s):  
Ryo Negishi ◽  
Kaori Takai ◽  
Tsuyoshi Tanaka ◽  
Tadashi Matsunaga ◽  
Tomoko Yoshino
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Digital Micromirror Device ◽  
Single Cell Encapsulation

High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device

2013 ◽  
Vol 35 (2-3) ◽  
pp. 385-392 ◽  
Author(s):  
Rogier M. Schoeman ◽  
Evelien W.M. Kemna ◽  
Floor Wolbers ◽  
Albert van den Berg
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

scholarly journals Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

2020 ◽  
Vol 92 (19) ◽  
pp. 13262-13270
Author(s):  
Kara K. Brower ◽  
Margarita Khariton ◽  
Peter H. Suzuki ◽  
Chris Still ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Double Emulsion ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

scholarly journals Hydro-Seq enables contamination-free high-throughput single-cell RNA-sequencing for circulating tumor cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu-Heng Cheng ◽  
Yu-Chih Chen ◽  
Eric Lin ◽  
Riley Brien ◽  
Seungwon Jung ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Rna Sequencing ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Single Cell Rna Sequencing

High-Throughput Generation of Durable Droplet Arrays for Single-Cell Encapsulation, Culture, and Monitoring

2018 ◽  
Vol 90 (7) ◽  
pp. 4303-4309 ◽  
Author(s):  
Han Wu ◽  
Xinlian Chen ◽  
Xinghua Gao ◽  
Mengying Zhang ◽  
Jinbo Wu ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

scholarly journals Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

2020 ◽  
Author(s):  
Kara K. Brower ◽  
Margarita Khariton ◽  
Peter H. Suzuki ◽  
Chris Still ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Single Cells ◽  
Double Emulsion ◽  
Cell Encapsulation ◽  
Functional Screening ◽  
Cellular Phenotype ◽  
Cell Loading ◽  
Single Cell Encapsulation

ABSTRACTIn the past five years, droplet microfluidic techniques have unlocked new opportunities for the high-throughput genome-wide analysis of single cells, transforming our understanding of cellular diversity and function. However, the field lacks an accessible method to screen and sort droplets based on cellular phenotype upstream of genetic analysis, particularly for large and complex cells. To meet this need, we developed Dropception, a robust, easy-to-use workflow for precise single-cell encapsulation into picoliter-scale double emulsion droplets compatible with high-throughput phenotyping via fluorescence-activated cell sorting (FACS). We demonstrate the capabilities of this method by encapsulating five standardized mammalian cell lines of varying size and morphology as well as a heterogeneous cell mixture of a whole dissociated flatworm (5 - 25 μm in diameter) within highly monodisperse double emulsions (35 μm in diameter). We optimize for preferential encapsulation of single cells with extremely low multiple-cell loading events (<2% of cell-containing droplets), thereby allowing direct linkage of cellular phenotype to genotype. Across all cell lines, cell loading efficiency approaches the theoretical limit with no observable bias by cell size. FACS measurements reveal the ability to discriminate empty droplets from those containing cells with good agreement to single-cell occupancies quantified via microscopy, establishing robust droplet screening at single-cell resolution. High-throughput FACS phenotyping of cellular picoreactors has the potential to shift the landscape of single-cell droplet microfluidics by expanding the repertoire of current nucleic acid droplet assays to include functional screening.ABSTRACT FIGURE

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