scholarly journals Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

2020 ◽  
Vol 92 (19) ◽  
pp. 13262-13270
Author(s):  
Kara K. Brower ◽  
Margarita Khariton ◽  
Peter H. Suzuki ◽  
Chris Still ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Double Emulsion ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

scholarly journals Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS

2020 ◽  
Author(s):  
Kara K. Brower ◽  
Margarita Khariton ◽  
Peter H. Suzuki ◽  
Chris Still ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Single Cells ◽  
Double Emulsion ◽  
Cell Encapsulation ◽  
Functional Screening ◽  
Cellular Phenotype ◽  
Cell Loading ◽  
Single Cell Encapsulation

ABSTRACTIn the past five years, droplet microfluidic techniques have unlocked new opportunities for the high-throughput genome-wide analysis of single cells, transforming our understanding of cellular diversity and function. However, the field lacks an accessible method to screen and sort droplets based on cellular phenotype upstream of genetic analysis, particularly for large and complex cells. To meet this need, we developed Dropception, a robust, easy-to-use workflow for precise single-cell encapsulation into picoliter-scale double emulsion droplets compatible with high-throughput phenotyping via fluorescence-activated cell sorting (FACS). We demonstrate the capabilities of this method by encapsulating five standardized mammalian cell lines of varying size and morphology as well as a heterogeneous cell mixture of a whole dissociated flatworm (5 - 25 μm in diameter) within highly monodisperse double emulsions (35 μm in diameter). We optimize for preferential encapsulation of single cells with extremely low multiple-cell loading events (<2% of cell-containing droplets), thereby allowing direct linkage of cellular phenotype to genotype. Across all cell lines, cell loading efficiency approaches the theoretical limit with no observable bias by cell size. FACS measurements reveal the ability to discriminate empty droplets from those containing cells with good agreement to single-cell occupancies quantified via microscopy, establishing robust droplet screening at single-cell resolution. High-throughput FACS phenotyping of cellular picoreactors has the potential to shift the landscape of single-cell droplet microfluidics by expanding the repertoire of current nucleic acid droplet assays to include functional screening.ABSTRACT FIGURE

High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device

2013 ◽  
Vol 35 (2-3) ◽  
pp. 385-392 ◽  
Author(s):  
Rogier M. Schoeman ◽  
Evelien W.M. Kemna ◽  
Floor Wolbers ◽  
Albert van den Berg
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

High-Throughput Generation of Durable Droplet Arrays for Single-Cell Encapsulation, Culture, and Monitoring

2018 ◽  
Vol 90 (7) ◽  
pp. 4303-4309 ◽  
Author(s):  
Han Wu ◽  
Xinlian Chen ◽  
Xinghua Gao ◽  
Mengying Zhang ◽  
Jinbo Wu ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

SINGLE CELL ENCAPSULATION IMPROVES STEM CELL RETENTION AND CELL-MEDIATED CARDIAC REPAIR

2014 ◽  
Vol 30 (10) ◽  
pp. S58 ◽  
Author(s):  
A.E. Mayfield ◽  
E.L. Tilokee ◽  
N. Latham ◽  
F. Rubens ◽  
D.W. Courtman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Encapsulation ◽  
Cardiac Repair ◽  
Cell Retention ◽  
Single Cell Encapsulation

Micropillar array embedded system for single cell encapsulation in hydrogel

2015 ◽  
Vol 1724 ◽  
Author(s):  
Kyun Joo Park ◽  
Kyoung G. Lee ◽  
Seunghwan Seok ◽  
Bong Gill Choi ◽  
Seok Jae Lee ◽  
...  
Keyword(s):  
Embedded System ◽  
Single Cell ◽  
Single Cells ◽  
Cell Encapsulation ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Practical Applications ◽  
Cell Clusters ◽  
Single Cell Encapsulation ◽  
Micropillar Array

ABSTRACTA cylindrical-shaped micropillar array embedded microfluidic device was proposed to enhance the dispersion of cell clusters and the efficiency of single cell encapsulation in hydrogel. Different sizes of micropillar arrays act as a sieve to break Escherichia coli (E. coli) aggregates into single cells in polyethylene glycol diacrylate (PEGDA) solution. We applied the external force for the continuous breakup of cell clusters, resulting in the production of more than 70% of single cells into individual hydrogel particles. This proposed strategy and device will be a useful platform to utilize genetically modified microorganisms in practical applications.

Peptide-catalyzed, bioinspired silicification for single-cell encapsulation in the imidazole-buffered system

2015 ◽  
Vol 51 (25) ◽  
pp. 5523-5525 ◽  
Author(s):  
Ji Hun Park ◽  
Insung S. Choi ◽  
Sung Ho Yang
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Yeast Cells ◽  
Mild Conditions ◽  
System P ◽  
Glass Sponges ◽  
Silica Encapsulation ◽  
Single Cell Encapsulation

Cytoprotective silica encapsulation of individual yeast cells is achieved by a peptide-catalyzed silicification under mild conditions, inspired by glass sponges.

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