Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures from Spatially Frequency Encoded1H NMR Spectra

2018 ◽  
Vol 90 (3) ◽  
pp. 1595-1600 ◽  
Author(s):  
Bertrand Plainchont ◽  
Daisy Pitoux ◽  
Mathieu Cyrille ◽  
Nicolas Giraud
Keyword(s):  
Quantitative Analysis ◽  
Nmr Spectra ◽  
Accurate Quantitative Analysis

Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

1982 ◽  
Vol 40 ◽  
pp. 332-333
Author(s):  
V. V. Damiano ◽  
R. P. Daniele ◽  
H. T. Tucker ◽  
J. H. Dauber
Keyword(s):  
Quantitative Analysis ◽  
Alveolar Macrophages ◽  
Bulk Sample ◽  
Silica Particles ◽  
Empirical Method ◽  
Phagocytic Cells ◽  
Calibration Standards ◽  
X Ray ◽  
Accurate Quantitative Analysis ◽  
Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

Biosensing Strategy for Simultaneous and Accurate Quantitative Analysis of Mycotoxins in Food Samples Using Unmodified Graphene Micromotors

2017 ◽  
Vol 89 (20) ◽  
pp. 10850-10857 ◽  
Author(s):  
Águeda Molinero-Fernández ◽  
María Moreno-Guzmán ◽  
Miguel Ángel López ◽  
Alberto Escarpa
Keyword(s):  
Quantitative Analysis ◽  
Food Samples ◽  
Accurate Quantitative Analysis

scholarly journals NmrLineGuru: Standalone and User-Friendly GUIs for Fast 1D NMR Lineshape Simulation and Analysis of Multi-State Equilibrium Binding Models

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chao Feng ◽  
Evgenii L. Kovrigin ◽  
Carol Beth Post
Keyword(s):  
Kinetic Model ◽  
Molecular Interactions ◽  
Rate Constants ◽  
Nmr Spectra ◽  
Equilibrium Constants ◽  
Software Tool ◽  
Biological Interactions ◽  
Equilibrium Binding ◽  
Accurate Quantitative Analysis ◽  
User Friendly

Abstract The ability of high-resolution NMR spectroscopy to readout the response of molecular interactions at multiple atomic sites presents a unique capability to define thermodynamic equilibrium constants and kinetic rate constants for complex, multiple-step biological interactions. Nonetheless, the extraction of the relevant equilibrium binding and rate constants requires the appropriate analysis of not only a readout that follows the equilibrium concentrations of typical binding titration curves, but also the lineshapes of NMR spectra. To best take advantage of NMR data for characterizing molecular interactions, we developed NmrLineGuru, a software tool with a user-friendly graphical user interface (GUI) to model two-state, three-state, and four-state binding processes. Application of NmrLineGuru is through stand-alone GUIs, with no dependency on other software and no scripted input. NMR spectra can be fitted or simulated starting with user-specified input parameters and a chosen kinetic model. The ability to both simulate and fit NMR spectra provides the user the opportunity to not only determine the binding parameters that best reproduce the measured NMR spectra for the selected kinetic model, but to also query the possibility that alternative models agree with the data. NmrLineGuru is shown to provide an accurate, quantitative analysis of complex molecular interactions.

Present and Emerging Techniques for Materials Microanalysis

1986 ◽  
Vol 69 ◽  
Author(s):  
C. R. Helms
Keyword(s):  
Quantitative Analysis ◽  
Spatial Resolution ◽  
High Sensitivity ◽  
Characterization Methods ◽  
Good Spatial Resolution ◽  
Accurate Quantitative Analysis ◽  
Significant Extension ◽  
Minimal Damage ◽  
A New Technique ◽  
The Cost

AbstractAlthough classical materials characterization methods have existed for many years, modern microanalytical techniques had their start just over twenty years ago. In this paper, I will discuss some of the common techniques available today including AES, XPS, or ESCA, RBS, SIMS, and EDAX. A comparison of the key capabilities and limitations will be given including sensitivity, spatial resolution, quantitative analysis, nondestructive testing, chemical state determination, and analysis speed. It is clear that the reason each of these techniques still exists as commercial instrumentation is that each provides a unique set of capabilities, but also a unique set of limitations. To become viable in the materials analysis arena, a new technique must offer a significant extension of the capabilities already available but not at the cost of too severe a set of limitations. Examples would be the development of tools that offer both high sensitivity with accurate quantitative analysis, or good spatial resolution with high sensitivity, or minimal damage but good spatial resolution, etc. A number of papers in this volume will describe the details of these emerging technologies which provide advances in these areas; and I will attempt here to put a number of these new developments in perspective with regard to the more commonplace techniques available.

Motor unit anatomy and physiology

2017 ◽  
Author(s):  
Matthew Pitt
Keyword(s):  
Quantitative Analysis ◽  
Motor Unit ◽  
Interference Pattern ◽  
Theoretical Explanation ◽  
Motor Unit Potential ◽  
Amplitude Analysis ◽  
Anatomy And Physiology ◽  
Needle Electromyography ◽  
Accurate Quantitative Analysis ◽  
Recruitment Order

This chapter focuses on the signals recorded with needle electromyography (EMG) and the measurement of their specific parameters. These parameters include duration, amplitude, number of phases, and stability. The concept of the electrophysiologic biopsy and the explanation of unusual findings seen on EMG are introduced. In relation to the interference pattern, discussions of the firing rate, recruitment order, and interference pattern are given. Moving from the theoretical explanation of the findings, the problems of the accurate quantitative analysis of the motor unit potential are discussed and measures to improve quantification, particularly in children, are highlighted. The importance of filter settings, the storage of signals, and the different ways of collecting and analysing the potentials are all covered. This section finishes with discussion of the normative range for motor unit duration, and concludes with the automatic analysis of the interference pattern, including turns/amplitude analysis, number of short segments measurement, and envelope analysis.

Quantitative Analysis Methods of Thiirane/Epoxy Resin of Bisphenol A

2006 ◽  
Vol 11-12 ◽  
pp. 379-382 ◽  
Author(s):  
Ya Feng Li ◽  
Jue Cheng
Keyword(s):  
Quantitative Analysis ◽  
Bisphenol A ◽  
Epoxy Resin ◽  
1H Nmr ◽  
Nmr Spectra ◽  
Epoxy Compound ◽  
Separation Method ◽  
The Poor ◽  
Epoxide Compound ◽  
Epoxy Groups

Thiirane is usually synthesized by epoxy resin, and there are considerable content of epoxy mixed in thiirane because of the poor yields. In this article, a novel separation method was used to get pure episulfide compound and epoxy compound from thiirane/epoxy resin synthesized in our lab. Furthermore, the perfect 1H NMR spectra of pure episulfide compound and epoxide compound were obtained. The quantitative analysis of content of epoxy groups in thiirane/epoxy resin was performed by using FTIR and was testified by 1H NMR. It was found that the analytical results of the conversion of epoxy groups by the two methods above coincided with each other well.

Quantitative analysis of hormones in cosmetics by LC-MS/MS combined with an advanced calibration model

2015 ◽  
Vol 7 (16) ◽  
pp. 6804-6809 ◽  
Author(s):  
Cai-Xia Shi ◽  
Zeng-Ping Chen ◽  
Yao Chen ◽  
Ru-Qin Yu
Keyword(s):  
Quantitative Analysis ◽  
Calibration Model ◽  
Signal Stability ◽  
Accurate Quantitative Analysis ◽  
Advanced Model

Accurate quantitative analysis of hormones in cosmetics was achieved through the combination of an advanced model with LC-MS/MS, even when significant variations occur in the sensitivity and signal stability of LC-MS/MS.

Sign in / Sign up

Export Citation Format

Share Document