Eight Hundred-Base Sequencing in a Microfabricated Electrophoretic Device

2000 ◽  
Vol 72 (14) ◽  
pp. 3388-3391 ◽  
Author(s):  
Lance Koutny ◽  
Dieter Schmalzing ◽  
Oscar Salas-Solano ◽  
Sameh El-Difrawy ◽  
Aram Adourian ◽  
...  
Keyword(s):  
Base Sequencing

A Base Specific Single Heavy Atom Stain for Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 184-185
Author(s):  
J. P. Langmore ◽  
N. R. Cozzarelli ◽  
A. V. Crewe
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
High Resolution ◽  
Elastic Scattering ◽  
Heavy Atom ◽  
High Vacuum ◽  
Heavy Atoms ◽  
Large Movement ◽  
Base Sequencing ◽  
Scanning Electron

A system has been developed to allow highly specific derivatization of the thymine bases of DNA with mercurial compounds wich should be visible in the high resolution scanning electron microscope. Three problems must be completely solved before this staining system will be useful for base sequencing by electron microscopy: 1) the staining must be shown to be highly specific for one base, 2) the stained DNA must remain intact in a high vacuum on a thin support film suitable for microscopy, 3) the arrangement of heavy atoms on the DNA must be determined by the elastic scattering of electrons in the microscope without loss or large movement of heavy atoms.

Molecular cloning and base sequencing of bovine enterovirus

1986 ◽  
Vol 14 (2) ◽  
pp. 472-472
Author(s):  
J. A. P. EARLE ◽  
R. A. SKUCE ◽  
E. M. HOEY ◽  
S. J. MARTIN
Keyword(s):  
Molecular Cloning ◽  
Bovine Enterovirus ◽  
Base Sequencing

Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast

1999 ◽  
Vol 45 (8) ◽  
pp. 686-690 ◽  
Author(s):  
Wouter J Middelhoven ◽  
Gloria Scorzetti ◽  
Jack W Fell
Keyword(s):  
Aromatic Compounds ◽  
Yeast Species ◽  
Large Subunit ◽  
Sole Source ◽  
Basidiomycetous Yeast ◽  
Phylogenetic Position ◽  
Base Sequencing

A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nuclear base sequencing of the D1/D2 region of the large subunit of rDNA is discussed.Key words: basidiomycetes, taxonomy, Trichosporon guehoae sp.nov., yeasts.

Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA

1994 ◽  
Vol 1209 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Karen P. McCann ◽  
Mohammed T. Akbari ◽  
Adrian C. Williams ◽  
David B. Ramsden
Keyword(s):  
Primary Structure ◽  
Type I ◽  
Cysteine Dioxygenase ◽  
Base Sequencing

Detection of virulent Escherichia coli O157 strains using multiplex PCR and single base sequencing for SNP characterization

2011 ◽  
Vol 110 (6) ◽  
pp. 1592-1600 ◽  
Author(s):  
K. Haugum ◽  
L.T. Brandal ◽  
I. Løbersli ◽  
G. Kapperud ◽  
B.-A. Lindstedt
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Escherichia Coli O157 ◽  
Single Base ◽  
Base Sequencing

scholarly journals Private haplotype barcoding facilitates inexpensive high-resolution genotyping of multiparent crosses

2017 ◽  
Author(s):  
Daniel A. Skelly ◽  
John H. McCusker ◽  
Eric A. Stone ◽  
Paul M. Magwene
Keyword(s):  
Markov Model ◽  
Hidden Markov Model ◽  
High Throughput Sequencing ◽  
Hidden Markov ◽  
Model Systems ◽  
Model Organisms ◽  
Large Numbers ◽  
Private Haplotype ◽  
Individual Offspring ◽  
Base Sequencing

AbstractInexpensive, high-throughput sequencing has led to the generation of large numbers of sequenced genomes representing diverse lineages in both model and non-model organisms. Such resources are well suited for the creation of new multiparent populations to identify quantitative trait loci that contribute to variation in phenotypes of interest. However, despite significant drops in per-base sequencing costs, the costs of sample handling and library preparation remain high, particularly when many samples are sequenced. We describe a novel method for pooled genotyping of offspring from multiple genetic crosses, such as those that that make up multiparent populations. Our approach, which we call "private haplotype barcoding” (PHB), utilizes private haplotypes to deconvolve patterns of inheritance in individual offspring from mixed pools composed of multiple offspring. We demonstrate the efficacy of this approach by applying the PHB method to whole genome sequencing of 96 segregants from 12 yeast crosses, achieving over a 90% reduction in sample preparation costs relative to non-pooled sequencing. In addition, we implement a hidden Markov model to calculate genotype probabilities for a generic PHB run and a specialized hidden Markov model for the yeast crosses that improves genotyping accuracy by making use of tetrad information. Private haplotype barcoding holds particular promise for facilitating inexpensive genotyping of large pools of offspring in diverse non-model systems.

Single-Base Sequencing and Similarity Comparisons

Genomics ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 445-449
Author(s):  
Guochun Xie ◽  
Rebecca Lobb ◽  
William J. Bruno ◽  
David C. Torney ◽  
Joe M. Gatewood
Keyword(s):  
Single Base ◽  
Base Sequencing
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