Stationary-Phase Contribution of 1-Propanol Organic Modifier to Changes in Sorption of 1-Hexanol on an ODS-Bonded Phase

2000 ◽  
Vol 72 (5) ◽  
pp. 1031-1038 ◽  
Author(s):  
Natalia Felitsyn ◽  
Frederick F. Cantwell
Keyword(s):  
Stationary Phase ◽  
Organic Modifier ◽  
Bonded Phase

Sulfated Polyborate, a novel buffer for low pH mobile phase on a non-end capped stationary phase in reverse phase liquid chromatography

2021 ◽  
Vol 08 ◽  
Author(s):  
Purushottam Sutar ◽  
Pravin Khedkar ◽  
Ganesh Chaturbhuj
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Basic Drugs ◽  
Buffer Concentration ◽  
Organic Modifier ◽  
Reverse Phase ◽  
Sulfated Polyborate ◽  
Reverse Phase Liquid Chromatography ◽  
Phase Liquid

Background: Sulfated Polyborate, a novel inorganic material primarily designed as a catalyst, has shown properties such as high solubility in organic solvents, low U.V. cut-off, and pKa ≈2.0, which suggests its potential as a mobile phase buffer for reverse-phase liquid chromatography. Objective: This study aims to substantiate the role of Sulfated Polyborate as mobile phase buffer for reverse-phase liquid chromatographic analysis of basic drugs with high pKa values viz. Bisoprolol fumarate, Timolol maleate, Verapamil hydrochloride, and Carvedilol. Methods: Solubilities, U.V. cut-offs, and pKa of Sulfated Polyborate was first experimentally confirmed. The behaviour of Sulfated Polyborate as mobile phase buffer at pH 3.0 was ascertained by varying the buffer concentration, flow rates, and percent organic modifier for elution of the four basic drugs on a non-end capped octyl silyl (C8) column. Similarly, the study was performed with KH2PO4 as a reference buffer. The column performance and conductometric measurements ascertained the impact of Sulfated Polyborate on the stationary phase. Results: Sulfated Polyborate and KH2PO4 buffers showed correlation coefficients of 0.99 and 1.00 for analyte retention factors for variation of buffer concentration and organic modifier composition, respectively. Peak symmetries and the number of theoretical plates were improved from > 2.0 to < 2.0 and ≈1000 to ≈3000, respectively, for Variation in buffer concentrations. Similar Van Deemter plots indicated equivalency of Sulfated Polyborate and KH2PO4 buffers. The column performance and conductometric measurements depicted no adsorption on the stationary phase. Conclusion: The present study demonstrates Sulfated Polyborate as a novel buffer for analytes with higher pKa on reverse-phase liquid chromatography.

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