Influence of the Hapten Design on the Development of a Competitive ELISA for the Determination of the Antifouling Agent Irgarol 1051 at Trace Levels

1998 ◽  
Vol 70 (19) ◽  
pp. 4004-4014 ◽  
Author(s):  
Berta Ballesteros ◽  
Damià Barceló ◽  
Francisco Sanchez-Baeza ◽  
Francisco Camps ◽  
María-Pilar Marco
Keyword(s):  
Competitive Elisa ◽  
Irgarol 1051 ◽  
Hapten Design ◽  
Antifouling Agent

An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters

1998 ◽  
Vol 32 (21) ◽  
pp. 3442-3447 ◽  
Author(s):  
Miguel Angel González-MartÍnez ◽  
José Penalva ◽  
Rosa Puchades ◽  
Angel Maquieira ◽  
Berta Ballesteros ◽  
...  
Keyword(s):  
Natural Waters ◽  
Automatic Determination ◽  
Irgarol 1051 ◽  
Antifouling Agent

scholarly journals Competitive ELISA for N-terminal pro-brain natriuretic peptide (NT-proBNP) determination in human plasma

The Analyst ◽  
2020 ◽  
Vol 145 (20) ◽  
pp. 6719-6727
Author(s):  
Glòria Colom ◽  
J.-Pablo Salvador ◽  
Gerardo Acosta ◽  
Fernando Albericio ◽  
Miriam Royo ◽  
...  
Keyword(s):  
Brain Natriuretic Peptide ◽  
Human Plasma ◽  
Natriuretic Peptide ◽  
Antibody Production ◽  
Competitive Elisa ◽  
Hapten Design

A competitive ELISA has been developed for the determination of NT-proBNP using a rational hapten design for its antibody production and also, for the development of the ELISA.

scholarly journals Determination of Apolipoprotein B-48 in Plasma by a Competitive ELISA

2000 ◽  
Vol 46 (10) ◽  
pp. 1638-1642 ◽  
Author(s):  
Anne-Marie Lorec ◽  
Christine Juhel ◽  
Yan Pafumi ◽  
Henri Portugal ◽  
Anne-Marie Pauli ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Healthy Subjects ◽  
Microtiter Plate ◽  
Cross Reactivity ◽  
Competitive Elisa ◽  
Plasma Samples ◽  
Postprandial State ◽  
Triton X ◽  
Suitable Marker

Abstract Background: Apolipoprotein B-48 (apoB-48) is produced by the small intestine, as part of chylomicrons, and appears to be a suitable marker for clinical studies of postprandial lipoproteins and related cardiovascular risk. Our aim was to develop, for routine analysis, an assay to quantify apoB-48 in plasma samples. Methods: A microtiter plate was coated with a C-terminal apoB-48-specific heptapeptide. Plasma samples were incubated with appropriate detergent to allow competition between immobilized antigen and plasma apoB-48. Appropriate calibration curves were obtained in the ELISA, using calibrated lymph and chylomicrons. Results: Treatment of plasma samples with the mild detergent Triton X-100 allowed an efficient competition between immobilized antigen and plasma apoB-48. No cross-reactivity was found with apoB-100, as checked by ELISA and Western blot analysis. Intra- and interassay CVs were 5.4% and 5.5%, respectively. In healthy subjects, apoB-48 concentrations markedly increased in the postprandial state, in parallel with triglycerides. Conclusions: This new ELISA allows determination of the concentration of apoB-48 in normolipidemic plasma.

Detection of total bile acids in biological samples using an indirect competitive ELISA based on four monoclonal antibodies

2017 ◽  
Vol 9 (4) ◽  
pp. 625-633 ◽  
Author(s):  
Shuchen Liu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Gaofeng Qin ◽  
Jinjun Cheng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clinical Practice ◽  
Biological Samples ◽  
Competitive Elisa ◽  
Routine Clinical Practice ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Different Types ◽  
Total Bile Acids

We investigated a newly developed indirect competitive enzyme-linked immunosorbent assay for the determination of 5 major components of TBA, which works efficiently in different types of biological samples, and may be suitable for routine clinical practice.

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