Improving the Activity of Immobilized Subtilisin by Site-Specific Attachment to Surfaces

1997 ◽  
Vol 69 (22) ◽  
pp. 4601-4607 ◽  
Author(s):  
Wei Huang ◽  
Jianquan Wang ◽  
Dibakar Bhattacharyya ◽  
Leonidas G. Bachas
Keyword(s):  
Site Specific ◽  
Specific Attachment ◽  
Immobilized Subtilisin

Site-Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone-Modified Phosphorothioate DNA

Small ◽  
2011 ◽  
Vol 7 (10) ◽  
pp. 1427-1430 ◽  
Author(s):  
Ngo Yin Wong ◽  
Chuan Zhang ◽  
Li Huey Tan ◽  
Yi Lu
Keyword(s):  
Site Specific ◽  
Specific Attachment ◽  
Dna Tetrahedron

Nano-Structured Beta-Gallia-Rutile Surfaces as Substrates for DNA Self-Assembly

2005 ◽  
Vol 901 ◽  
Author(s):  
Nathan Empie ◽  
Doreen Edwards
Keyword(s):  
Self Assembly ◽  
Divalent Cations ◽  
Dna Adsorption ◽  
Site Specific ◽  
Force Microscopy ◽  
Atomic Force ◽  
Site Selectivity ◽  
Afm Imaging ◽  
Specific Attachment ◽  
Mode Atomic Force Microscopy

AbstractA nano-structured beta-gallia-rutile (BGR) substrate capable of binding DNA was synthesized. Beta-gallia groups diffuse into [001] single crystal rutile along {210}r planes, generating hexagonally shaped tunnel sites between the beta-gallia subunits (repeating ∼1 nm). The tunnel sites, approximately 2.5 Å in diameter, are preferred regions for cation incorporation. Divalent cations have been used previously to adsorb DNA to mica surfaces. For the BGR system, the site selectivity of the cations for tunnel sites could lead to controllable / tailor-able DNA adsorption. DNA buffers containing Cu (II), Fe (II), and Ni (II) cations were deposited on BGR substrates. The DNA adsorption was investigated with tapping mode atomic force microscopy (AFM) to determine the suitability of using BGR substrates as a means to self assemble DNA constructs for nano-electronic applications. At the concentrations tested, only solutions containing Ni (II) ions were capable of binding DNA sufficiently for AFM imaging; there was no evidence of site specific attachment.

Site-Specific Attachment of Polyethylene Glycol-like Oligomers to Proteins and Peptides

2006 ◽  
Vol 17 (6) ◽  
pp. 1492-1498 ◽  
Author(s):  
Yoann Marsac ◽  
Janina Cramer ◽  
Diana Olschewski ◽  
Kirill Alexandrov ◽  
Christian F. W. Becker
Keyword(s):  
Polyethylene Glycol ◽  
Site Specific ◽  
Specific Attachment ◽  
Proteins And Peptides

Evolution of genomic islands by deletion and tandem accretion by site-specific recombination: ICESt1-related elements from Streptococcus thermophilus

Microbiology ◽  
2004 ◽  
Vol 150 (4) ◽  
pp. 759-774 ◽  
Author(s):  
Guillaume Pavlovic ◽  
Vincent Burrus ◽  
Brigitte Gintz ◽  
Bernard Decaris ◽  
Gérard Guédon
Keyword(s):  
Streptococcus Thermophilus ◽  
Genomic Islands ◽  
Recombination Site ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Attachment Sites ◽  
Specific Attachment ◽  
Circular Molecule

The 34 734-bp integrative and potentially conjugative element (putative ICE) ICESt1 has been previously found to be site-specifically integrated in the 3′ end of the fda locus of Streptococcus thermophilus CNRZ368. Four types of genomic islands related to ICESt1 are integrated in the same position in seven other strains of S. thermophilus. One of these elements, ICESt3, harbours conjugation and recombination modules closely related to those of ICESt1 and excises by site-specific recombination. Two other types of elements, CIME19258 and CIME302, are flanked by site-specific attachment sites closely related to attL and attR of ICESt1 and ICESt3, whereas ΔCIME308 only possesses a putative attR site; none of these three elements carry complete conjugation and recombination modules. ICESt1 contains a functional internal recombination site, attL′, that is almost identical to attL of CIME19258. The recombination between attL′ and attR of ICESt1 leads to the excision of the expected circular molecule (putative ICE); a cis-mobilizable element (CIME) flanked by an attL site and an attB′ site remains integrated into the 3′ end of fda. Furthermore, sequences that could be truncated att sites were found within ICESt1, ICESt3 and CIME302. All together, these data suggest that these genomic islands evolved by deletion and tandem accretion of ICEs and CIMEs resulting from site-specific recombination. A model for this evolution is proposed and its application to other genomic islands is discussed.

Site-Specific Attachment of a Protein to a Carbon Nanotube End without Loss of Protein Function

2012 ◽  
Vol 23 (7) ◽  
pp. 1488-1493 ◽  
Author(s):  
Shige H. Yoshimura ◽  
Shahbaz Khan ◽  
Satoshi Ohno ◽  
Takashi Yokogawa ◽  
Kazuya Nishikawa ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Protein Function ◽  
Site Specific ◽  
Specific Attachment

Use of a thiol tether for the site-specific attachment of reporter groups to DNA

1992 ◽  
Vol 57 (8) ◽  
pp. 2340-2346 ◽  
Author(s):  
Jacqueline A. Fidanza ◽  
Larry W. McLaughlin
Keyword(s):  
Site Specific ◽  
Specific Attachment
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