Site-Specific Attachment of Polyethylene Glycol-like Oligomers to Proteins and Peptides

2006 ◽  
Vol 17 (6) ◽  
pp. 1492-1498 ◽  
Author(s):  
Yoann Marsac ◽  
Janina Cramer ◽  
Diana Olschewski ◽  
Kirill Alexandrov ◽  
Christian F. W. Becker
Keyword(s):  
Polyethylene Glycol ◽  
Site Specific ◽  
Specific Attachment ◽  
Proteins And Peptides

The Impact of Bioconjugation on the Interfacial Activity of a Protein Biosurfactant

2018 ◽  
Author(s):  
Hossam H Tayeb ◽  
Marina Stienecker ◽  
Anton Middelberg ◽  
Frank Sainsbury
Keyword(s):  
Polyethylene Glycol ◽  
Colloidal Stability ◽  
Point Mutations ◽  
Pharmaceutical Formulations ◽  
Industrial Processes ◽  
Parent Molecule ◽  
Site Specific ◽  
Interfacial Activity ◽  
Surface Active ◽  
The Impact

Biosurfactants, are surface active molecules that can be produced by renewable, industrially scalable biologic processes. DAMP4, a designer biosurfactant, enables the modification of interfaces via genetic or chemical fusion to functional moieties. However, bioconjugation of addressable amines introduces heterogeneity that limits the precision of functionalization as well as the resolution of interfacial characterization. Here we designed DAMP4 variants with cysteine point mutations to allow for site-specific bioconjugation. The DAMP4 variants were shown to retain the structural stability and interfacial activity characteristic of the parent molecule, while permitting efficient and specific conjugation of polyethylene glycol (PEG). PEGylation results in a considerable reduction on the interfacial activity of both single and double mutants. Comparison of conjugates with one or two conjugation sites shows that both the number of conjugates as well as the mass of conjugated material impacts the interfacial activity of DAMP4. As a result, the ability of DAMP4 variants with multiple PEG conjugates to impart colloidal stability on peptide-stabilized emulsions is reduced. We suggest that this is due to constraints on the structure of amphiphilic helices at the interface. Specific and efficient bioconjugation permits the exploration and investigation of the interfacial properties of designer protein biosurfactants with molecular precision. Our findings should therefore inform the design and modification of biosurfactants for their increasing use in industrial processes, and nutritional and pharmaceutical formulations.

Site-Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone-Modified Phosphorothioate DNA

Small ◽  
2011 ◽  
Vol 7 (10) ◽  
pp. 1427-1430 ◽  
Author(s):  
Ngo Yin Wong ◽  
Chuan Zhang ◽  
Li Huey Tan ◽  
Yi Lu
Keyword(s):  
Site Specific ◽  
Specific Attachment ◽  
Dna Tetrahedron

Nano-Structured Beta-Gallia-Rutile Surfaces as Substrates for DNA Self-Assembly

2005 ◽  
Vol 901 ◽  
Author(s):  
Nathan Empie ◽  
Doreen Edwards
Keyword(s):  
Self Assembly ◽  
Divalent Cations ◽  
Dna Adsorption ◽  
Site Specific ◽  
Force Microscopy ◽  
Atomic Force ◽  
Site Selectivity ◽  
Afm Imaging ◽  
Specific Attachment ◽  
Mode Atomic Force Microscopy

AbstractA nano-structured beta-gallia-rutile (BGR) substrate capable of binding DNA was synthesized. Beta-gallia groups diffuse into [001] single crystal rutile along {210}r planes, generating hexagonally shaped tunnel sites between the beta-gallia subunits (repeating ∼1 nm). The tunnel sites, approximately 2.5 Å in diameter, are preferred regions for cation incorporation. Divalent cations have been used previously to adsorb DNA to mica surfaces. For the BGR system, the site selectivity of the cations for tunnel sites could lead to controllable / tailor-able DNA adsorption. DNA buffers containing Cu (II), Fe (II), and Ni (II) cations were deposited on BGR substrates. The DNA adsorption was investigated with tapping mode atomic force microscopy (AFM) to determine the suitability of using BGR substrates as a means to self assemble DNA constructs for nano-electronic applications. At the concentrations tested, only solutions containing Ni (II) ions were capable of binding DNA sufficiently for AFM imaging; there was no evidence of site specific attachment.

scholarly journals Structure-Function Studies of Interleukin 15 using Site-specific Mutagenesis, Polyethylene Glycol Conjugation, and Homology Modeling

1997 ◽  
Vol 272 (4) ◽  
pp. 2312-2318 ◽  
Author(s):  
Dean K. Pettit ◽  
Timothy P. Bonnert ◽  
June Eisenman ◽  
Subhashini Srinivasan ◽  
Ray Paxton ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Homology Modeling ◽  
Structure Function ◽  
Site Specific ◽  
Interleukin 15

Site-Specific Immunosuppression using a New Formulation of Topical Cyclosporine A with Polyethylene Glycol-8 Glyceryl Caprylate/Caprate

1999 ◽  
Vol 83 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Hoang S. Tran ◽  
Dipak Malli ◽  
Francis A. Chrzanowski ◽  
Matthew M. Puc ◽  
Martha S. Matthews ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Cyclosporine A ◽  
Site Specific ◽  
New Formulation ◽  
Specific Immunosuppression
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