Characterization of Visible Dyes for Four-Decay Fluorescence Detection in DNA Sequencing

1997 ◽  
Vol 69 (13) ◽  
pp. 2392-2397 ◽  
Author(s):  
Brian K. Nunnally ◽  
Hui He ◽  
Liang-Chi Li ◽  
Sheryl A. Tucker ◽  
Linda B. McGown
Keyword(s):  
Dna Sequencing ◽  
Fluorescence Detection

scholarly journals SPECTRUM OF BETA GLOBIN GENE MUTATIONS IN EGYPTIAN CHILDREN WITH β- THALASSEMIA

2014 ◽  
Vol 6 (1) ◽  
pp. e2014071 ◽  
Author(s):  
MR El-Shanshory ◽  
Adel Abd Elhaleim Hagag
Keyword(s):  
Dna Sequencing ◽  
Blood Count ◽  
Complete Blood Count ◽  
Globin Gene ◽  
Gene Mutations ◽  
Thalassemia Major ◽  
Direct Dna Sequencing ◽  
Rare Mutations ◽  
Egyptian Children

Background: The molecular defects resulting in β-thalassemia phenotype, in the Egyptian population show a clear heterogenic mutations pattern. PCR based techniques, including direct DNA sequencing are effective on the molecular detection and characterization of these mutations. The molecular characterization of β-thalassemia is absolutely necessary for carrier screening, for genetic counseling, and to offer prenatal diagnosis.The aim of the work: was to evaluate the different β-globin gene mutations in one hundred Egyptian children with β-thalassemia. Patients and Methods: One hundred of β-thalassemic Egyptian children, covering most Egyptian Governorates. All patients were subjected to meticulous history taking, clinical examinations, complete blood count, complete blood count, hemoglobin electrophoresis, serum ferritin and direct fluorescent DNA sequencing of β-globin gene to detect the frequency of different mutations in studied patients. Results: The most common mutations among patients were IVS I-110(G>A) 48%, IVS I-6(T>C) 40%, IVS I-1(G>A)19%,IVS I-5(G>C)10%, IVS II-848 (C>A) 9%, IVS II-745(C>G) 8%, IVS II-1(G>A) 7%, codon"Cd"39(C> T) 4%,-87(C>G) 3% and the rare mutations were: Cd37 (G>A), Cd8 (-AA), Cd29(-G), Cd5 (-CT), Cd6(-A), Cd8/9(+G), Cd 106/107(+G), Cd27(C>T), IVS II-16(G> C), Cd 28 (-C), Cap+1(A>C), -88(C>A), all of these rare mutations were present in 1%. There was considerable variation in phenotypic severity among patients resulting from interaction of different β° and β+mutations, 79(79%) patients were thalassemia major (TM) and 21(21%) were thassemia intermedia (TI), without genotype phenotype association. Conclusion: Direct DNA sequencing provides insights for the frequency of different mutations in β- thalassemic patients including rare and /or unknown ones.

scholarly journals Synthesis and characterization of MOFs constructed from 5-(benzimidazole-1-yl)isophthalic acid and highly selective fluorescence detection of Fe(iii) and Cr(vi) in water

RSC Advances ◽  
2020 ◽  
Vol 10 (57) ◽  
pp. 34943-34952
Author(s):  
Bin Zhu ◽  
Guimei Huang ◽  
Yanni He ◽  
Jisheng Xie ◽  
Tao He ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Isophthalic Acid ◽  
Synthesis And Characterization ◽  
Solvothermal Conditions

In this work, four novel MOFs [Cd(bipa)]n (1), {[Zn2(bipa)2]·2C2H5OH}n (2), {[Co(bipa)]·C2H5OH}n (3), {[Ni(bipa)2]·2DMA}n (4), (H2bipa = 5-(benzimidazole-1-yl)isophthalic acid) were successfully synthesized under solvothermal conditions.

Joining forces: integrating the mechanical and optical single molecule toolkits

2014 ◽  
Vol 5 (5) ◽  
pp. 1680-1697 ◽  
Author(s):  
Monique J. Jacobs ◽  
Kerstin Blank
Keyword(s):  
Fluorescence Detection ◽  
Single Molecule ◽  
Materials Science ◽  
Force Measurements

Combining single molecule force measurements with fluorescence detection opens up exciting new possibilities for the characterization of mechanoresponsive molecules in Biology and Materials Science.

Characterization of Hb djeifa [β98(FG5)Val→Ala] by DNA sequencing in a French caucasian family

Hemoglobin ◽  
1999 ◽  
Vol 23 (1) ◽  
pp. 73-77 ◽  
Author(s):  
P. Lacan ◽  
M. Aubry ◽  
A. Francina ◽  
N. Couprie ◽  
L. Dementhon ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Caucasian Family

scholarly journals Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation

2014 ◽  
Vol 13 (1) ◽  
pp. 1304-1313 ◽  
Author(s):  
C.S. Kodama ◽  
S. Cuadros-Orellana ◽  
C.H.M.M. Bandeira ◽  
D.A. Graças ◽  
A.S. Santos ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Fluorescence Detection ◽  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

scholarly journals Color-blind fluorescence detection for four-color DNA sequencing

2005 ◽  
Vol 102 (15) ◽  
pp. 5346-5351 ◽  
Author(s):  
E. K. Lewis ◽  
W. C. Haaland ◽  
F. Nguyen ◽  
D. A. Heller ◽  
M. J. Allen ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Fluorescence Detection ◽  
Color Blind
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