Chemiluminescence Enzyme Immunoassay Using Bacterial Magnetic Particles

1996 ◽  
Vol 68 (20) ◽  
pp. 3551-3554 ◽  
Author(s):  
Tadashi Matsunaga ◽  
Masashi Kawasaki ◽  
Xie Yu ◽  
Noriyuki Tsujimura ◽  
Noriyuki Nakamura
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Particles ◽  
Bacterial Magnetic Particles ◽  
Chemiluminescence Enzyme Immunoassay

Evaluation of a magnetic particles-based chemiluminescence enzyme immunoassay for Golgi protein 73 in human serum

2015 ◽  
Vol 445 ◽  
pp. 54-59 ◽  
Author(s):  
Xiangyi Liu ◽  
Xiaohua Wan ◽  
Sheng Lu ◽  
Lijun Zhang ◽  
Shaohua Yu ◽  
...  
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
Magnetic Particles ◽  
Golgi Protein ◽  
Chemiluminescence Enzyme Immunoassay

Development of a sensitive magnetic particles chemiluminescence enzyme immunoassay for determination of aflatoxin B1 in corn

2011 ◽  
Vol 41 (7) ◽  
pp. 1177-1183 ◽  
Author(s):  
Hui CHEN ◽  
Jin-Ming LIN ◽  
XiTang YING ◽  
GuoMao HU ◽  
GuoJin ZHENG
Keyword(s):  
Enzyme Immunoassay ◽  
Aflatoxin B1 ◽  
Magnetic Particles ◽  
Chemiluminescence Enzyme Immunoassay

A magnetic particles-based chemiluminescence enzyme immunoassay for rapid detection of ovalbumin

2014 ◽  
Vol 459 ◽  
pp. 12-17 ◽  
Author(s):  
Xiao-Li Feng ◽  
Hong-Lin Ren ◽  
Yan-Song Li ◽  
Pan Hu ◽  
Yu Zhou ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Magnetic Particles ◽  
Chemiluminescence Enzyme Immunoassay

scholarly journals Assembly of G Protein-Coupled Receptors onto Nanosized Bacterial Magnetic Particles Using Mms16 as an Anchor Molecule

2004 ◽  
Vol 70 (5) ◽  
pp. 2880-2885 ◽  
Author(s):  
Tomoko Yoshino ◽  
Masayoshi Takahashi ◽  
Haruko Takeyama ◽  
Yoshiko Okamura ◽  
Fukuichi Kato ◽  
...  
Keyword(s):  
G Protein ◽  
Magnetic Particles ◽  
Lipid Membrane ◽  
Specific Protein ◽  
G Protein Coupled Receptors ◽  
Native Conformation ◽  
Wide Range ◽  
Bacterial Magnetic Particles ◽  
Magnetospirillum Magneticum ◽  
G Protein Coupled

ABSTRACT G protein-coupled receptors (GPCRs) play a central role in a wide range of biological processes and are prime targets for drug discovery. GPCRs have large hydrophobic domains, and therefore purification of GPCRs from cells is frequently time-consuming and typically results in loss of native conformation. In this work, GPCRs have been successfully assembled into the lipid membrane of nanosized bacterial magnetic particles (BMPs) produced by the magnetic bacterium Magnetospirillum magneticum AMB-1. A BMP-specific protein, Mms16, was used as an anchor molecule, and localization of heterologous Mms16 on BMPs was confirmed by luciferase fusion studies. Stable luminescence was obtained from BMPs bearing Mms16 fused with luciferase at the C-terminal region. D1 dopamine receptor (D1R), a GPCR, was also efficiently assembled onto BMPs by using Mms16 as an anchor molecule. D1R-BMP complexes were simply extracted by magnetic separation from ruptured AMB-1 transformants. After washing, the complexes were ready to use for analysis. This system conveniently refines the native conformation of GPCRs without the need for detergent solubilization, purification, and reconstitution after cell disruption.

Development of high-performance magnetic chemiluminescence enzyme immunoassay for α-fetoprotein (AFP) in human serum

2008 ◽  
Vol 393 (2) ◽  
pp. 90-94 ◽  
Author(s):  
Xu Wang ◽  
Qian-Yun Zhang ◽  
Zhen-Jia Li ◽  
Xi-Tang Ying ◽  
Jin-Ming Lin
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
High Performance ◽  
Chemiluminescence Enzyme Immunoassay
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