scholarly journals Dual-labeled chemiluminescence enzyme immunoassay for simultaneous measurement of total prostate specific antigen (TPSA) and free prostate specific antigen (FPSA)

Luminescence ◽  
2017 ◽  
Vol 32 (8) ◽  
pp. 1547-1553 ◽  
Author(s):  
Lixia Zhao ◽  
Dan Wang ◽  
Gen Shi ◽  
Ling Lin
Keyword(s):  
Enzyme Immunoassay ◽  
Prostate Specific Antigen ◽  
Simultaneous Measurement ◽  
Specific Antigen ◽  
Total Prostate Specific Antigen ◽  
Chemiluminescence Enzyme Immunoassay

Cumulative Prostate Cancer Risk Assessment with the Aid of the Free-to-Total Prostate Specific Antigen Ratio

2004 ◽  
Vol 45 (2) ◽  
pp. 160-165 ◽  
Author(s):  
Gunnar Aus ◽  
Charlotte Becker ◽  
Stefan Franzén ◽  
Hans Lilja ◽  
Pär Lodding ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Risk Assessment ◽  
Cancer Risk ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Prostate Cancer Risk ◽  
Cancer Risk Assessment ◽  
Total Prostate Specific Antigen

Five-Year Stability Study of Free and Total Prostate-Specific Antigen Concentrations in Serum Specimens Collected and Stored at – 70°C or Less

2007 ◽  
Vol 22 (3) ◽  
pp. 206-213 ◽  
Author(s):  
D.A. Scaramuzzino ◽  
K. Schulte ◽  
B.N. Mack ◽  
T.F. Soriano ◽  
H.A. Fritsche
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Stability Study ◽  
Regression Analyses ◽  
Altman Analysis ◽  
Time Period ◽  
Total Prostate Specific Antigen ◽  
Deming Regression ◽  
Lower Temperature ◽  
The Stability

The stability of total (t) and free (f) prostate-specific antigen (PSA) in male serum specimens stored at –70°C or lower temperature for 4.7 to 4.9 years was studied. Until now, the stability of these analytes in serum has not been evaluated systematically beyond 2 years of storage at –70°C. Aliquots of frozen serum were thawed in 2001 and 2006 and assayed for tPSA and fPSA using a Dade Behring Dimension(r) RxL analyzer and reagents. tPSA values ranged from 0.07 to 69.94 and 0.00 to 69.83 ng/mL in 2001 and 2006, respectively, whereas fPSA values for the tested specimens ranged from 0.02 to 5.72 and 0.00 to 5.92, respectively. Deming regression analyses showed agreement in assay values over time as tPSA values yielded a slope of 1.0112 and a y-intercept of 0.0195; fPSA values produced a slope 1.0538 and a y-intercept of –0.0442; f/tPSA values yielded a slope of 0.9631 and a y-intercept of 0.1195. A Bland-Altman analysis of the data demonstrated analyte and ratio stability over this time period. We conclude that serum, when collected properly and stored at –70°C or lower temperature, may be used for tPSA and fPSA clinical studies for at least 5 years after collection.

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