scholarly journals Metabolomic Analysis via Reversed-Phase Ion-Pairing Liquid Chromatography Coupled to a Stand Alone Orbitrap Mass Spectrometer

2010 ◽  
Vol 82 (8) ◽  
pp. 3212-3221 ◽  
Author(s):  
Wenyun Lu ◽  
Michelle F. Clasquin ◽  
Eugene Melamud ◽  
Daniel Amador-Noguez ◽  
Amy A. Caudy ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Mass Spectrometer ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Metabolomic Analysis ◽  
Orbitrap Mass Spectrometer

Development of a Method for the Determination of Pyrimethamine Concentrations in Feeds by Ion-Pairing High-Performance Liquid Chromatography

2002 ◽  
Vol 65 (4) ◽  
pp. 688-691 ◽  
Author(s):  
P. S. GONG ◽  
S. L. JENG ◽  
Y. F. HSU ◽  
C. C. LIN ◽  
S. Y. LIN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Hplc Method ◽  
Alumina Column ◽  
Tetramethylammonium Chloride ◽  
Array Detection

An ion-pairing reversed-phase high-performance liquid chromatography (HPLC) method with diode array detection at 280 nm was developed to determine pyrimethamine concentrations in feed for laying hens. Pyrimethamine was extracted with a mixture of 5% isobutanol and 95% benzene, and the extract was cleaned up on an alumina column. The drug was eluted from an Intersil ODS-3V column (250 by 4.6 mm) with a mixture of 25% acetonitrile and 75% water (vol/vol) containing 0.01 M tetramethylammonium chloride as an ion-pairing agent and adjusted with acetic acid to pH 3.5. The flow rate was 1.0 ml/min. Mean recovery of pyrimethamine from supplemented feeds at concentrations of 2, 4, and 5 μg/g of feed were 100.5, 103.5, and 100.8%, respectively. Precision within a day ranged from 4.3 to 7.0% for the three concentrations, and day-to-day precision was 5.3% for feed supplemented at a concentration of 4 μg/g. No chromatographic interference was detected from other 2,4-diaminopyrimidine compounds or other major drugs used in poultry.

scholarly journals Styryllactones and Acetogenins from the Fruits of Goniothalamus macrocalyx

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Quy Hung Trieu ◽  
Huong Doan Thi Mai ◽  
Van Cuong Pham ◽  
Marc Litaudon ◽  
Françoise Gueritte ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Cell Lines ◽  
Mass Spectrometer ◽  
Natural Resource ◽  
Absolute Configuration ◽  
Mcf7 Cell ◽  
Crystallographic Analysis ◽  
Kb Cells ◽  
X Ray ◽  
Orbitrap Mass Spectrometer

Two new styryllactones, macrocalactone (1) and 3-deoxycardiobutanolide (2), were isolated from the fruits of Goniothalamus macrocalyx Ban (Annonaceae), together with seven known compounds including four acetogenins, annonacin (3), solamin (4), isoannonacin (5), trans-murisolinone (6), and three other compounds, 7-acetylaltholactone (7), β-caryophyllene-8R,9R-oxide (8) and 2-(2′-hydroxytetracosanoylamino)-octadecane-l,3,4-triol (9). Their structures were determined by spectroscopic and MS analysis. The absolute configuration of I was determined by X-ray crystallographic analysis. The structures of the acetogenins were confirmed by liquid chromatography coupled to a hybrid quadrupole-time of flight mass spectrometer, using post-column lithium infusion. The results were compared with the fragmentation obtained with a hybrid linear trap-orbitrap mass spectrometer. Compound 7 had cytotoxicity against KB, HepG2, Lu, and MCF7 cell lines with IC50 values of 13.1, 23.7, 26.3 and 60.2 μM, respectively, whereas annonacin (3) was selectively active against KB cells (IC50 value of 6.5 μM). The discovery of 3-deoxycardiobutanolide (2) from the fruits of this plant revealed that G. macrocalyx could be a valuable natural resource to obtain this compound as it has been previously reported to have a significant cytotoxicity against different cancer cell lines, especially HL-60 cells.

Sign in / Sign up

Export Citation Format

Share Document