Speciation of [PtIVCl6-nBrn]2- (n = 0-6) and some of their mono-aquated [PtIVCl5-nBrn(H2O)]- (n = 0-5) anions in solution at low concentrations by means of ion-pairing reversed-phase ultra-high-performance liquid chromatography coupled to electrospray ion

2014 ◽  
Vol 28 (5) ◽  
pp. 505-519 ◽  
Author(s):  
Pieter-Hugo van Wyk ◽  
Jacobus B. van Dyk ◽  
Wilhemus J. Gerber ◽  
Marietjie Stander ◽  
André de Villiers ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Low Concentrations

Development of a Method for the Determination of Pyrimethamine Concentrations in Feeds by Ion-Pairing High-Performance Liquid Chromatography

2002 ◽  
Vol 65 (4) ◽  
pp. 688-691 ◽  
Author(s):  
P. S. GONG ◽  
S. L. JENG ◽  
Y. F. HSU ◽  
C. C. LIN ◽  
S. Y. LIN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Hplc Method ◽  
Alumina Column ◽  
Tetramethylammonium Chloride ◽  
Array Detection

An ion-pairing reversed-phase high-performance liquid chromatography (HPLC) method with diode array detection at 280 nm was developed to determine pyrimethamine concentrations in feed for laying hens. Pyrimethamine was extracted with a mixture of 5% isobutanol and 95% benzene, and the extract was cleaned up on an alumina column. The drug was eluted from an Intersil ODS-3V column (250 by 4.6 mm) with a mixture of 25% acetonitrile and 75% water (vol/vol) containing 0.01 M tetramethylammonium chloride as an ion-pairing agent and adjusted with acetic acid to pH 3.5. The flow rate was 1.0 ml/min. Mean recovery of pyrimethamine from supplemented feeds at concentrations of 2, 4, and 5 μg/g of feed were 100.5, 103.5, and 100.8%, respectively. Precision within a day ranged from 4.3 to 7.0% for the three concentrations, and day-to-day precision was 5.3% for feed supplemented at a concentration of 4 μg/g. No chromatographic interference was detected from other 2,4-diaminopyrimidine compounds or other major drugs used in poultry.

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