Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics

2009 ◽  
Vol 81 (15) ◽  
pp. 6030-6037 ◽  
Author(s):  
Andreas Lenshof ◽  
Asilah Ahmad-Tajudin ◽  
Kerstin Järås ◽  
Ann-Margret Swärd-Nilsson ◽  
Lena Åberg ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Specific Antigen

scholarly journals Measurement of Prostate-specific Antigen by Use of a Novel Blood Collection and Analytical System

2002 ◽  
Vol 48 (8) ◽  
pp. 1272-1278 ◽  
Author(s):  
Barbara R Grzeda ◽  
Tuan Le Bui ◽  
Cheryl N Warner ◽  
Tracy L Pirucki ◽  
Lisa M Dewey ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Collection ◽  
Serum Samples ◽  
Stabilizing Solution ◽  
Professional Assistance ◽  
Comparison Of The Results

Abstract Background: Prostate-specific antigen (PSA) is widely used in the detection and monitoring of prostate cancer. We developed a system for the self-collection and transport of capillary whole blood for PSA analysis, with the goal of reducing phlebotomy visits and, thus, increasing the access and utilization of PSA in prostate cancer screening and monitoring. Methods: The blood collection device [BIOSAFE Blood Transport System (BTSTM)] collects 70 μL of blood through a heparin-coated material into 200 μL of stabilizing solution. The diluted whole blood is used for measurement of PSA by a modified version of the Hybritech® Tandem-MP PSA Assay. Results were compared for matched samples of professionally and self-collected BTS blood and for matched BTS samples sera from blood collected by venipuncture. Imprecision for the whole-blood PSA measurement was estimated from analysis of whole-blood controls in duplicate, twice per day, over 20 days. Results: BTS samples (n = 140) collected by a qualified healthcare professional compared with serum samples yielded the regression equation: y =1.02x + 0.04 (Sy|x = 0.35; r = 0.99). Comparison of the results for samples (n = 128) collected by the patient without professional assistance with serum samples yielded: y = 1.08x + 0.02 (Sy|x = 0.31; r = 0.99). The between-run CVs at 0.069, 0.53, 2.9, and 10.7 μg/L were 21%, 6.0%, 3.5%, and 3.8%, respectively. PSA was stable in BTS samples stored for 21 days at 18–24 °C and for 7 days at 37 °C. Conclusion: The BIOSAFE BTS system allows accurate and convenient measurement of circulating PSA by a precise method for diluted whole blood.

scholarly journals Assay of prostate-specific antigen from whole blood spotted on filter paper and application to prostate cancer screening

1996 ◽  
Vol 42 (4) ◽  
pp. 536-544 ◽  
Author(s):  
B R Hoffman ◽  
H Yu ◽  
E P Diamandis
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Whole Blood ◽  
Large Scale ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Concentrations ◽  
Hot Climate ◽  
Plasma Fraction

Abstract We report the measure of prostate-specific antigen (PSA) from extracts of blood dried on filter paper. Five 3-mm (diameter) paper discs containing approximately 25 microL of dried whole blood were punched from the filter paper and extracted with 500 microL of buffer. Recovery of PSA was > 92%. Imprecision of the filter paper procedure was <10% when corresponding whole-blood concentrations were >0.35 micrograms/L. PSA recovery was unaffected whether blood was applied to the filter as one 85-microL aliquots, two 43-microL aliquots, or three 28-microL aliquots. PSA is contained in the plasma fraction. Variation in hematocrit from 0.61 to 0.31 caused <+/-10% change in filter paper PSA. Regression analysis showed: filter paper PSA = 0.86 whole-blood PSA - 0.02; Sy/x = 0.44. Men (153) without prostate cancer gave a 95th percentile of 4.8 micrograms /L. PSA in filter paper dried blood was stable for >1 month at -20 to 37 degrees C and showed no loss of recovery after being mailed to a hot climate. We conclude that the filter paper procedure can reliably distinguish normal from increased concentrations of PSA and that it could facilitate screening to detect occult prostate cancer in large-scale mail-in programs to centralized laboratories.

Rapid one-step assay for prostate specific antigen in whole blood using an optical immunoassay technology

1995 ◽  
Author(s):  
Grenville Robinson ◽  
Timothy Bacarese-Hamilton ◽  
Paul M. O'Neill ◽  
Janys E. Fletcher ◽  
Phelim B. Daniels ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Specific Antigen ◽  
One Step

scholarly journals A novel split mode TFBAR device for quantitative measurements of prostate specific antigen in a small sample of whole blood

Nanoscale ◽  
2020 ◽  
Vol 12 (17) ◽  
pp. 9647-9652
Author(s):  
Ewelina Wajs ◽  
Girish Rughoobur ◽  
Keith Burling ◽  
Anne George ◽  
Andrew J. Flewitt ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Point Of Care ◽  
Effective Means ◽  
Specific Antigen ◽  
Cost Effective ◽  
Small Sample ◽  
Quantitative Measurements ◽  
Point Of Care Diagnostics ◽  
Split Mode

TFBAR technology demonstrates a cost-effective means for point-of-care diagnostics and monitoring of PSA.

scholarly journals Measurement of Circulating Forms of Prostate-specific Antigen in Whole Blood Immediately after Venipuncture: Implications for Point-of-Care Testing

2001 ◽  
Vol 47 (4) ◽  
pp. 703-711 ◽  
Author(s):  
Timo Piironen ◽  
Martti Nurmi ◽  
Kerttu Irjala ◽  
Olli Heinonen ◽  
Hans Lilja ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Point Of Care ◽  
Prostate Gland ◽  
Specific Antigen ◽  
Point Of Care Testing ◽  
Blood Samples ◽  
Whole Blood Samples

Abstract Background: The purpose of this study was to validate the use of whole-blood samples in the determination of circulating forms of prostate-specific antigen (PSA). Methods: Blood samples of hospitalized prostate cancer and benign prostatic hyperplasia patients were collected and processed to generate whole-blood and serum samples. Three different rapid two-site immunoassays were developed to measure the concentrations of total PSA (PSA-T), free PSA (PSA-F), and PSA-α1-antichymotrypsin complex (PSA-ACT) to detect in vitro changes in whole-blood samples immediately after venipuncture. The possible influence of muscle movement on the release of PSA from prostate gland was studied in healthy men by measuring the rapid in vitro whole-blood kinetics of PSA forms before and after 15 min of physical exercise on a stationary bicycle. Results: Rapid PSA-T, PSA-F, and PSA-ACT assays were designed using a 10-min sample incubation. No significant changes were detected in the concentrations of PSA-T, PSA-F, and PSA-ACT from the earliest time point of 12–16 min compared with measurements performed up to 4 h after venipuncture. Physical exercise did not influence the concentrations of the circulating forms of PSA. Hematocrit-corrected whole-blood values of PSA-T and PSA-F forms were comparable to the respective serum values. Calculation of the percentage of PSA-F (PSA F/T ratio × 100) was similar irrespective of the sample format used, i.e., whole blood or serum. Conclusions: We found that immunodetectable PSA forms are likely at steady state immediately after venipuncture, thus enabling the use of anticoagulated whole-blood samples in near-patient settings for point-of-care testing, whereas determinations of PSA (e.g., PSA-T, PSA-F, or PSA-ACT) performed within the time frame of the office visit would provide results equivalent to conventional analyses performed in serum.

A comparison of three fluorophores for use in an optical biosensor for the measurement of prostate-specific antigen in whole blood

1995 ◽  
Vol 27 (1-3) ◽  
pp. 447-451 ◽  
Author(s):  
P.B. Daniels ◽  
J.E. Fletcher ◽  
P.M. O'Neill ◽  
C.G. Stafford ◽  
T. Bacarese-Hamilton ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Specific Antigen ◽  
Optical Biosensor

Semi-Quantitative Immunochromatographic Test for Prostate Specific Antigen in Whole Blood: Tossing the Coin to Predict Prostate Cancer?

2003 ◽  
Vol 43 (5) ◽  
pp. 478-484 ◽  
Author(s):  
Frank Oberpenning ◽  
Stefan Hetzel ◽  
Christoph Weining ◽  
Burkhard Brandt ◽  
Gabriela De Angelis ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Specific Antigen ◽  
Immunochromatographic Test

scholarly journals A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood

Lab on a Chip ◽  
2014 ◽  
Vol 14 (16) ◽  
pp. 2918-2928 ◽  
Author(s):  
Ana I. Barbosa ◽  
Ana P. Castanheira ◽  
Alexander D. Edwards ◽  
Nuno M. Reis
Keyword(s):  
Prostate Specific Antigen ◽  
Human Blood ◽  
Whole Blood ◽  
Point Of Care ◽  
Specific Antigen ◽  
Psa Screening

A “lab-in-a-briefcase” for point-of-care PSA screening that can perform 80 measurements of PSA levels in human blood in 15 minutes.

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