Integration of Dialysis Membranes into a Poly(dimethylsiloxane) Microfluidic Chip for Isoelectric Focusing of Proteins Using Whole-Channel Imaging Detection

2008 ◽  
Vol 80 (19) ◽  
pp. 7401-7407 ◽  
Author(s):  
Junjie Ou ◽  
Tomasz Glawdel ◽  
Razim Samy ◽  
Shuwen Wang ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Dialysis Membranes ◽  
Imaging Detection

Free Flow Isoelectric Focusing in a Microfluidic Device

2014 ◽  
Author(s):  
Kisoo Yoo ◽  
Prashanta Dutta ◽  
Jin Liu
Keyword(s):  
Mathematical Model ◽  
Isoelectric Focusing ◽  
Microfluidic Device ◽  
Microfluidic Chip ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Free Flow ◽  
Charged Species ◽  
Simulation Results ◽  
Separation Of Proteins

In recent years, there are growing interests in the use of free flow isoelectric focusing (FFIEF). In FFIEF, a thin sheath of laminar flow is introduced perpendicular to the direction of the applied electric field for continuous separation of proteins and charged species. This technique is especially useful in microfluidic device since the electrophoretically separated bands do not have to be mobilized for detection or further analysis. In this study, a mathematical model is developed to simulate free flow isoelectric process in microfluidic devices considering electroneutrality and incompressibility of electrolytes. Our mathematical model is based on mass, momentum and charge conservation equations. A finite volume based numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results indicate that pH gradient forms as samples flow downstream and proteins can be separated effectively using this technique. A new design of microfluidic chip is proposed for separation for cardiac troponin I from serum albumin using FFIEF technique.

Multistage Isoelectric Focusing: A Novel On-Chip Bio-Separation Technique

2005 ◽  
Author(s):  
Prashanta Dutta ◽  
Keisuke Horiuchi ◽  
Huanchun Cui ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Fluorescent Protein ◽  
Ph Gradient ◽  
Resolving Power ◽  
Protein Species ◽  
Second Stage ◽  
On Chip ◽  
Bonding Technique

This experimental study reports a method to increase the resolving power of isoelectric focusing (IEF) on a polymeric microfluidic chip. Microfluidic chip is formed on poly-di-methyl siloxane (PDMS) using soft lithography and multilayer bonding technique. In this novel bioseparation technique, IEF is staged by first focusing protein species in a straight channel using broad-range ampholytes and then refocusing segments of that first channel into secondary channels that branch out from the first one. Experiments demonstrated that three fluorescent protein species within a segment of pH gradient in the first stage were refocused in the second stage with much higher resolution in a shallower pH gradient. A serially performed two-stage IEF was completed in less than 25 minutes under particularly small electric field strength up to 100 V/cm.

Fluorescence imaging detection for capillary isoelectric focusing

1995 ◽  
Vol 16 (1) ◽  
pp. 1474-1478 ◽  
Author(s):  
Xing-Zheng Wu ◽  
Jiaqi Wu ◽  
Janusz Pawliszyn
Keyword(s):  
Fluorescence Imaging ◽  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
Imaging Detection
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