One-Step Label-Free Optical Genosensing System for Sequence-Specific DNA Related to the Human Immunodeficiency Virus Based on the Measurements of Light Scattering Signals of Gold Nanorods

2008 ◽  
Vol 80 (22) ◽  
pp. 8424-8430 ◽  
Author(s):  
Wei He ◽  
Cheng Zhi Huang ◽  
Yuan Fang Li ◽  
Jian Ping Xie ◽  
Rong Ge Yang ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Light Scattering ◽  
Gold Nanorods ◽  
Label Free ◽  
Immunodeficiency Virus ◽  
One Step

scholarly journals Dynamic Host Energetics and Cytoskeletal Proteomes in Human Immunodeficiency Virus Type 1-Infected Human Primary CD4 Cells: Analysis by Multiplexed Label-Free Mass Spectrometry

2009 ◽  
Vol 83 (18) ◽  
pp. 9283-9295 ◽  
Author(s):  
Eric Y. Chan ◽  
Jennifer N. Sutton ◽  
Jon M. Jacobs ◽  
Andrey Bondarenko ◽  
Richard D. Smith ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Immunodeficiency Virus ◽  
Virus Replication ◽  
Mass Spectrometry Analysis ◽  
Protein Abundance ◽  
Label Free ◽  
Cd4 Cells ◽  
Hiv Replication ◽  
Immunodeficiency Virus

ABSTRACT We report on a proteomic analysis of ex vivo human immunodeficiency virus (HIV) type 1 infection in human primary CD4 cells by shotgun liquid chromatography-tandem mass spectrometry analysis, revealing two distinct proteomic profiles at two phases of virus replication. Relative to mock-infected cells, 168 signature proteins exhibited abundance changes at the first sign of Gag p24 production (8 h postinfection [p.i.]) or the peak of virus replication (24 h p.i.); interestingly, most of the changes were exclusive to only one phase of virus replication. Based on characterization by functional ontology and known human-HIV protein interactions, we observed the enrichment for protein abundance increases pertaining to protein synthesis and nucleasomal reorganization amid an otherwise placid cellular proteome at the first sign of HIV replication. In contrast, we observed indications of decreased protein turnover, concomitant with heightened DNA repair activities and preludes to apoptosis, in the presence of robust virus replication. We also observed hints of disruptions in protein and small molecule trafficking. Our label-free proteomic strategy allowed us to perform multiplexed comparisons—we buttressed our detection specificity with the use of a reverse transcriptase inhibitor as a counterscreen, enabling highlighting of cellular protein abundance changes unique to robust virus replication as opposed to viral entry. In conjunction with complementary high-throughput screens for cellular partners of HIV, we put forth a model pinpointing specific rerouting of cellular biosynthetic, energetic, and trafficking pathways as HIV replication accelerates in human primary CD4 cells.

A new label-free fluorescent sensor for human immunodeficiency virus detection based on exonuclease III-assisted quadratic recycling amplification and DNA-scaffolded silver nanoclusters

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2998-3003 ◽  
Author(s):  
Wen Yang ◽  
Jianniao Tian ◽  
Lijun Wang ◽  
Shui Fu ◽  
Hongyun Huang ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Fluorescent Sensor ◽  
Dna Detection ◽  
Virus Detection ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Hiv Dna ◽  
Immunodeficiency Virus ◽  
Recycling Amplification

A label-free and sensitive fluorescence biosensing platform for HIV-DNA detection has been fabricated.

scholarly journals Proteome and Protein Network Analyses of Memory T Cells Find Altered Translation and Cell Stress Signaling in Treated Human Immunodeficiency Virus Patients Exhibiting Poor CD4 Recovery

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Sausan Azzam ◽  
Daniela Schlatzer ◽  
Sean Maxwell ◽  
Xiaolin Li ◽  
Douglas Bazdar ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Memory T Cells ◽  
Protein Translation ◽  
Selected Reaction Monitoring ◽  
Label Free ◽  
Cell Recovery ◽  
Network Analyses ◽  
Immunodeficiency Virus ◽  
Related Proteins

Abstract Background.  Human immunodeficiency virus (HIV) patients who experience poor CD4 T-cell recovery despite viral suppression during antiretroviral therapy (ART) are known as immunological nonresponders. The molecular mechanism(s) underlying incomplete immune restoration during ART is not fully understood. Methods.  Label-free quantitative proteomics on single-cell type central memory T cells were used to reveal relative protein abundance changes between nonresponder, responder (good CD4 recovery during ART), and healthy individuals. Proteome changes were analyzed by protein pathway and network analyses and verified by selected reaction monitoring mass spectrometry. Results.  Proteomic analysis across groups detected 155 significant proteins from 1500 nonredundant proteins. Pathway and network analyses revealed dysregulation in mammalian target of rapamycin and protein translation-related proteins and decreases in stress response-related proteins for nonresponder subjects compared with responders and controls. Actin cytoskeleton signaling was increased for HIV responders and nonresponders alike. Conclusions.  Memory T cells from immunologic nonresponders have increases in proteins related to motility and protein translation and decreases in proteins capable of responding to cellular stresses compared with responders and controls. The potential for T cells to manage stress and modulate metabolism may contribute to their capacity to reconstitute a lymphopenic host.

Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods

2020 ◽  
Author(s):  
Hong Zhang ◽  
Pengfei Liu ◽  
Huifang Wang ◽  
Xiaoming Ji ◽  
Mingqin Zhao ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Fluorescent Sensor ◽  
Label Free ◽  
One Step ◽  
Positively Charged
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