Refractive index anomalies in stopped-flow measurements

1976 ◽  
Vol 48 (4) ◽  
pp. 778-779 ◽  
Author(s):  
Mark L. Miller ◽  
Gilbert. Gordon
Keyword(s):  
Refractive Index ◽  
Stopped Flow ◽  
Flow Measurements

Stopped-flow measurements of CO and O2 uptake by hemoglobin in sheep erythrocytes

1963 ◽  
Vol 18 (1) ◽  
pp. 158-165 ◽  
Author(s):  
John A. Sirs ◽  
F. J. W. Roughton
Keyword(s):  
Linear Relation ◽  
Flow System ◽  
Stopped Flow ◽  
Constant Flow ◽  
Dissolved Gas ◽  
O2 Uptake ◽  
Flow Measurements ◽  
Sheep Erythrocytes ◽  
Flow Method ◽  
Comparison Of The Results

A stopped-flow method is described to measure spectrophotometrically the rates of uptake of CO and O2 by the hemoglobin of intact sheep erythrocytes. A comparison of the results obtained with the constant flow system has led to further refinement of the latter procedure. A linear relation is found between the rate of uptake and the concentration of the dissolved gas, and the initial percentage rate of saturation is independent of the concentration of the erythrocytes. Submitted on September 21, 1961

The Origin of Aeolian Dunes – PIV measurements of flow structure over early stage protodunes in a refractive-index-matching flume

2020 ◽  
Author(s):  
Nathaniel Bristow ◽  
James Best ◽  
Kenneth Christensen ◽  
Matthew Baddock ◽  
Giles Wiggs ◽  
...  
Keyword(s):  
Refractive Index ◽  
Flow Field ◽  
Turbulent Flow ◽  
Fixed Bed ◽  
Field Measurements ◽  
Morphological Development ◽  
Flow Measurements ◽  
Early Stages ◽  
Aeolian Dunes ◽  
Turbulent Flow Field

<p>Understanding the initiation of aeolian dunes poses significant challenges due to the strong couplings between turbulent fluid flow, sediment transport, and bedform morphology. While much is known concerning the dynamics of more mature bedforms, open questions remain as to how protodunes are formed, as well as the mechanisms by which they continue to evolve. The structure of the turbulent flow field drives the mobilization or deposition of sediment, thus controlling the initial formation of sand patches, yet is also strongly influenced itself by local conditions such as surface roughness and moisture. Furthermore, an additional feedback on the flow and transport is exerted by the sand patches themselves once they begin to form.</p><p>As protodunes begin to develop from this initial deposition, their morphologies possess unique characteristics involving a reverse asymmetry of the stoss and lee sides, wherein the crest begins upstream, close to the toe, and gradually shifts downstream toward the "regular" asymmetric profile exhibited by more mature dunes. However, these early stages of development also involve very gentle slopes and low profiles which make field measurements of the associated flow particularly challenging.</p><p>The current research effort involves a combination of field measurements, documenting the initiation and morphological development of sand patches and protodunes, in concert with detailed measurements of the flow-form interactions in a laboratory flume. The work presented herein focuses primarily on experiments conducted in a unique flow facility wherein high-resolution measurements of the turbulent flow field associated with the early stages of protodune development are obtained utilizing particle-image velocimetry (PIV) in a refractive-index-matched (RIM) environment. The RIM technique facilitates flow measurements extremely close to model surfaces as well as unimpeded optical access which are critical to understanding the flow-form coupling. A series idealized, fixed-bed models are fabricated to mimic the key morphological characteristics of early protodune development observed in the field, and the flow measurements associated with them are analyzed to reveal the mechanisms controlling the bedform dynamics.</p>

Kinetics and Equilibrium of Binding of Fe3+ by a Fulvic Acid: A Study by Stopped Flow Methods

1975 ◽  
Vol 53 (20) ◽  
pp. 2979-2984 ◽  
Author(s):  
Cooper H. Langford ◽  
Tahir R. Khan
Keyword(s):  
Ionic Strength ◽  
Complex Formation ◽  
Fulvic Acid ◽  
Metal Ion ◽  
Equilibrium Constants ◽  
Acid Dissociation ◽  
Stopped Flow ◽  
Solution Ph ◽  
Sulfosalicylic Acid ◽  
Flow Measurements

The first report of a rate of binding of a metal ion (Fe3+) by a soluble fulvic acid is derived from stopped flow measurements. The rate of complex formation is normal in Wilkins' sense and similar to that for sulfosalicylic acid. Dissociation is slow (t1/2 > 10 s). The binding of Fe3+ by the fulvic acid in acid solution, pH = 1–2.5, was investigated by kinetic analysis in which the reaction of free Fe3+ with sulfosalicylic acid was followed by stopped flow spectrophotometry on a time scale short compared to release of Fe3+ by fulvic acid. Conditional equilibrium constants found were 1.5 ± 0.3 × 104 at pH = 1.5 and 2.5, and 2.8 ± 0.3 × 103 at pH = 1.0 at 25 °C (ionic strength 0.1).

scholarly journals Studies by electron-paramagnetic-resonance spectroscopy and stopped-flow spectrophotometry on the mechanism of action of turkey liver xanthine dehydrogenase

1976 ◽  
Vol 153 (2) ◽  
pp. 297-307 ◽  
Author(s):  
M J Barber ◽  
R C Bray ◽  
D J Lowe ◽  
M P Coughlan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Redox Potential ◽  
Active Sites ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Xanthine Dehydrogenase ◽  
Resonance Spectroscopy ◽  
Stopped Flow ◽  
Paramagnetic Resonance ◽  
Flow Measurements ◽  
Electron Paramagnetic

Studies by e.p.r. (electron-paramagnetic-resonance) spectroscopy and by stopped-flow spectrophotometry on turkey liver xanthine dehydrogenase revealed strong similarities to as well as important differences from the Veillonella alcalescens xanthine dehydrogenase and milk xanthine oxidase. The turkey enzyme is contaminated by up to three non-functional forms, giving molybdenum e.p.r. signals designated Resting I, Resting II and Slow. Slow and to a lesser extent Resting I signals are like those from the Veillonella enzyme, whereas Resting II is very like a resting signal described by K. V. Rajagopolan, P. Handler, G. Palmer & H. Beinert (1968) (J. Biol. Chem. 243, 3784-3796) for aldehyde oxidase. Another non-functional form that gives the Inhibited signal is produced on treatment of the enzyme with formaldehyde. Stopped-flow measurements at 450 nm show that, as for the milk enzyme, reduction by xanthine is rate-limiting in enzyme turnover. The active enzyme gives rise to Very Rapid and Rapid molybdenum(V) e.p.r. signals, as well as to an FADH signal. That these signals are almost indistinguishable from those of the milk enzyme, confirms the similarities between the active sites. There are two types of iron-sulphur centres that give signals like those in the milk enzyme, though with slightly different parameters. Quantitative reduction titration of the functional enzyme with xanthine revealed two important differences between the turkey and the milk enzymes. First, the turkey enzyme FADH/FADH2 system has a redox potential sufficiently low that xanthine is incapable of reducing the flavin completely. This finding presumably explains the very low oxidase activity. Secondly, whereas the Fe/S II chromophore in the milk enzyme has a relatively high redox potential, for the turkey enzyme the value of this potential is lower and similar to that of its Fe/S I chromophore.

scholarly journals Kinetic studies on the oxidation of nitrite by horseradish peroxidase and lactoperoxidase.

1999 ◽  
Vol 46 (4) ◽  
pp. 919-927 ◽  
Author(s):  
L Gebicka
Keyword(s):  
Horseradish Peroxidase ◽  
Nitrogen Dioxide ◽  
Rate Constants ◽  
Kinetic Studies ◽  
The Other ◽  
Stopped Flow ◽  
Nitrite Concentration ◽  
Flow Measurements ◽  
Activity Measurements ◽  
Compound Ii

The reaction of nitrite (NO2-) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing stopped-flow measurements gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 13.3 +/- 0.07 mol(-1) dm3 s(-1) and 3.5 +/- 0.05 x 10(4) mol(-1) dm3 s(-1), respectively. Nitrite, at neutral pH, influenced measurements of activity of lactoperoxidase with typical substrates like 2,2'-azino-bis[ethyl-benzothiazoline-(6)-sulphonic acid] (ABTS), guaiacol or thiocyanate (SCN-). The rate of ABTS and guaiacol oxidation increased linearly with nitrite concentration up to 2.5-5 mmol dm(-3). On the other hand, two-electron SCN- oxidation was inhibited in the presence of nitrite. Thus, nitrite competed with the investigated substrates of lactoperoxidase. The intermediate, most probably nitrogen dioxide (*NO2), reacted more rapidly with ABTS or guaiacol than did lactoperoxidase compound II. It did not, however, effectively oxidize SCN- to OSCN-. NO2- did not influence the activity measurements of horseradish peroxidase by ABTS or guaiacol method.

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