Example of flame photometric analysis for methyl parathion in rat whole blood and brain tissue

1971 ◽  
Vol 43 (8) ◽  
pp. 1102-1105 ◽  
Author(s):  
Joe. Gabica ◽  
Joe. Wyllie ◽  
Michael. Watson ◽  
W. W. Benson
Keyword(s):  
Brain Tissue ◽  
Whole Blood ◽  
Methyl Parathion ◽  
Photometric Analysis

Example of Flame Photometric Analysis for Methyl Parathion in Rat Whole Blood and Brain Tissue.

1971 ◽  
Vol 43 (12) ◽  
pp. 1633-1633
Author(s):  
Joe. Gabica ◽  
Joe. Wyllie ◽  
Michael. Watson ◽  
W. W. Benson
Keyword(s):  
Brain Tissue ◽  
Whole Blood ◽  
Methyl Parathion ◽  
Photometric Analysis

scholarly journals Arterio-venous differences of choline and choline lipids across the brain of rat and rabbit

1976 ◽  
Vol 154 (1) ◽  
pp. 133-140 ◽  
Author(s):  
S Spanner ◽  
R C Hall ◽  
G B Ansell
Keyword(s):  
Brain Tissue ◽  
Whole Blood ◽  
Blood Cells ◽  
Jugular Vein ◽  
Arterial Supply ◽  
The Brain

The concentration of unesterified choline in the plasma in the jugular vein of the rat (0.85 nmol/ml) was found to be three times that of the arterial supply to the brain (0.25 nmol/ml), indicating a higher efflux than uptake of unesterified choline by the brain. No such difference was found for the rabbit and no arterio-venous difference for phosphatidylcholine or lysophosphatidylcholine was observed in either species. No arterio-venous difference was found for choline in blood cells. The infusion of [Me-3H]choline into the circulation of the rat or rabbit indicated an uptake of radioactive choline by the brain and an efflux of non-radioactive choline. In the rabbit such an infusion produced a steady rise in the labelling of phosphatidylcholine and lysophosphatidylcholine in the plasma. When [14C2]ethanolamine was injected intraperitoneally into the rat there was a labelling of phosphatidylcholine, lysophosphatidylcholine and sphingomyelin in the plasma and cells of blood from the jugular vein and the arterial supply, as well as in the brain tissue. However, no labelling of unesterified choline in these tissues could be detected. Unesterified choline was shown to be liberated into the plasma when whole blood from the rat or man, but not the rabbit, was incubated for short periods at 30 degrees C.

scholarly journals Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
João Bosco de Salles ◽  
Renato Matos Lopes ◽  
Cristiane M. C. de Salles ◽  
Vicente P. F. Cassano ◽  
Manildo Marcião de Oliveira ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Methyl Parathion ◽  
Tap Water ◽  
Freshwater Fishes ◽  
The Other ◽  
Organophosphate Pesticide ◽  
Ache Inhibition ◽  
Prochilodus Lineatus ◽  
Piaractus Mesopotamicus ◽  
Brain Ache

Three species of freshwater Brazilian fishes (pacu,Piaractus mesopotamicus; piavussu,Leporinus macrocephalus, and curimbatá,Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of allP. lineatusat 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

Letter: Whole blood volume determinations

1974 ◽  
Vol 134 (1) ◽  
pp. 181b-181
Author(s):  
R. E. Willard
Keyword(s):  
Blood Volume ◽  
Whole Blood
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