scholarly journals Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
João Bosco de Salles ◽  
Renato Matos Lopes ◽  
Cristiane M. C. de Salles ◽  
Vicente P. F. Cassano ◽  
Manildo Marcião de Oliveira ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Methyl Parathion ◽  
Tap Water ◽  
Freshwater Fishes ◽  
The Other ◽  
Organophosphate Pesticide ◽  
Ache Inhibition ◽  
Prochilodus Lineatus ◽  
Piaractus Mesopotamicus ◽  
Brain Ache

Three species of freshwater Brazilian fishes (pacu,Piaractus mesopotamicus; piavussu,Leporinus macrocephalus, and curimbatá,Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of allP. lineatusat 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

UV ability to inactivate microorganisms combined with factors affecting radiation

1997 ◽  
Vol 35 (11-12) ◽  
pp. 107-112 ◽  
Author(s):  
A. M. Shaban ◽  
G. E. El-Taweel ◽  
G. H. Ali
Keyword(s):  
Microbial Communities ◽  
Uv Radiation ◽  
Contact Time ◽  
Tap Water ◽  
Scenedesmus Obliquus ◽  
Morphological Characters ◽  
The Other ◽  
Bacterial Cells ◽  
Dark Repair ◽  
Nile Water

In the present study, the effect of UV radiation on the inactivation of a range of microorganisms was studied. Each organism was seeded into sterile tap water and exposed to UV in batch experiments with changing turbidities. In addition, the effect of UV on microbial communities in river Nile water was examined. It was found that 1min contact time (0.5L/min flow rate) was effective against vegetative cells levels almost reaching zero (except with Staphylococcus aureus). On the other hand, spore-forming bacteria, Candida albicans and coliphage were more resistant to UV. This contact time caused coenobia cells in single form with Scenedesmus obliquus while for Microcystis aeruginosa colonies broke into smaller groups. Exposure of Nile water microbial communities to UV showed that yeasts and Aeromonas survived better than the other organisms while in the phytoplankton partial fragmentation occurred in some algal groups. The protective effect of turbidity differed between organisms, with increased contact time under conditions of stable turbidity having no effect on the organisms. At 20 NTU the UV radiation had no effect on the morphological characters of algal cells. In reactivation experiments, it is clear that photoreactivation, and not dark repair, takes place with bacterial cells. Only coliphage had no photoreactivation and dark repair responses although with coliphage and host, both reactivation processes worked well. Moreover, the irradiated algae regained their normal shape after 3 days in suitable media and enough light.

scholarly journals Polyphenol Profiling of Chestnut Pericarp, Integument and Curing Water Extracts to Qualify These Food By-Products as a Source of Antioxidants

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2335
Author(s):  
Gabriella Pinto ◽  
Sabrina De Pascale ◽  
Maria Aponte ◽  
Andrea Scaloni ◽  
Francesco Addeo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Negative Impact ◽  
Tap Water ◽  
Time Of Flight ◽  
The Other ◽  
Curing Process ◽  
Flight Mass Spectrometry ◽  
Water Curing ◽  
The Impact ◽  
By Products

Plant polyphenols have beneficial antioxidant effects on human health; practices aimed at preserving their content in foods and/or reusing food by-products are encouraged. The impact of the traditional practice of the water curing procedure of chestnuts, which prevents insect/mould damage during storage, was studied to assess the release of polyphenols from the fruit. Metabolites extracted from pericarp and integument tissues or released in the medium from the water curing process were analyzed by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray-quadrupole-time of flight-mass spectrometry (ESI-qTOF-MS). This identified: (i) condensed and hydrolyzable tannins made of (epi)catechin (procyanidins) and acid ellagic units in pericarp tissues; (ii) polyphenols made of gallocatechin and catechin units condensed with gallate (prodelphinidins) in integument counterparts; (iii) metabolites resembling those reported above in the wastewater from the chestnut curing process. Comparative experiments were also performed on aqueous media recovered from fruits treated with processes involving: (i) tap water; (ii) tap water containing an antifungal Lb. pentosus strain; (iii) wastewater from a previous curing treatment. These analyses indicated that the former treatment determines a 6–7-fold higher release of polyphenols in the curing water with respect to the other ones. This event has a negative impact on the luster of treated fruits but qualifies the corresponding wastes as a source of antioxidants. Such a phenomenon does not occur in wastewater from the other curing processes, where the release of polyphenols was reduced, thus preserving the chestnut’s appearance. Polyphenol profiling measurements demonstrated that bacterial presence in water hampered the release of pericarp metabolites. This study provides a rationale to traditional processing practices on fruit appearance and qualifies the corresponding wastes as a source of bioactive compounds for other nutraceutical applications.

Example of Flame Photometric Analysis for Methyl Parathion in Rat Whole Blood and Brain Tissue.

1971 ◽  
Vol 43 (12) ◽  
pp. 1633-1633
Author(s):  
Joe. Gabica ◽  
Joe. Wyllie ◽  
Michael. Watson ◽  
W. W. Benson
Keyword(s):  
Brain Tissue ◽  
Whole Blood ◽  
Methyl Parathion ◽  
Photometric Analysis

Voluntary dehydration and alliesthesia for water

1984 ◽  
Vol 57 (3) ◽  
pp. 868-873 ◽  
Author(s):  
R. W. Hubbard ◽  
B. L. Sandick ◽  
W. T. Matthew ◽  
R. P. Francesconi ◽  
J. B. Sampson ◽  
...  
Keyword(s):  
Tap Water ◽  
The Other ◽  
Heat Illness ◽  
Complex Relationship ◽  
Fluid Consumption ◽  
Severe Dehydration ◽  
Apparent Paradox ◽  
Weight Losses ◽  
Voluntary Dehydration ◽  
Thirst Mechanism

The purpose of this experiment was to explore the complex relationship between fluid consumption and consumption factors (thirst, voluntary dehydration, water alliesthesia, palatability, work-rest cycle) during a simulated 14.5-km desert walk (treadmill, 1.34 m X s-1, 5% grade, 40 degrees C dry bulb/26 degrees C wet bulb, and wind speed of approximately 1.2 m X s-1). Twenty-nine subjects were tested (30 min X h-1, 6 h) on each of two nonconsecutive days. The subjects were randomly assigned to one of three groups: tap water (n = 8), iodine-treated tap water (n = 11), or iodine-treated flavored tap water (n = 10). The temperature of the water was 40 degrees C during one trial and 15 degrees C on the other. Mean sweat losses (6 h) varied between 1.4 kg (warm iodine-treated; 232 +/- 44 g X h-1) and 3.0 kg (cool iodine-treated flavored; 509 +/- 50 g X h-1). Warm drinks were consumed at a lower rate than cool drinks (negative and positive alliesthesia). This decreased consumption resulted in the highest percent body weight losses (2.8 and 3.2%). Cooling and flavoring effects on consumption were additive and increased the rate of intake by 120%. The apparent paradox between reduced consumption concomitant with severe dehydration and hyperthermia is attributed to negative alliesthesia for warm water rather than an apparent inadequacy of the thirst mechanism. The reluctance to drink warm iodine-treated water resulted in significant hyperthermia, dehydration, hypovolemia, and, in two cases, heat illness.

scholarly journals Technological, chemical, sensory, and microbiological examination of frozen chicken as affected by microwave thawing

2005 ◽  
Vol 21 (1-2) ◽  
pp. 99-108 ◽  
Author(s):  
M.A. Kenawi
Keyword(s):  
Ambient Temperature ◽  
Tap Water ◽  
Thiobarbituric Acid ◽  
Acid Value ◽  
Bacterial Count ◽  
The Other ◽  
Total Bacterial Count ◽  
Running Water ◽  
Thiobarbituric Acid Value ◽  
Microbiological Properties

The effect of microwave heating as a thawing method on physical, chemical sensory, and microbiological properties of frozen chicken was investigated in comparison with other thawing methods (at ambient temperature, in refrigerator, and in running tap water). Microwave thawed chicken had the highest taste panel scores and the lowest drip percentage loss compared with the other thawing methods. Thiobarbituric acid value (TBA) remarkably increased the samples thawed at ambient temperature or under running water. The data revealed that the retention of thiamin was the highest in the microwave thawed samples (97.33%), and the lowest in running water thawed samples (66.66%). The total bacterial count in frozen chicken remarkably decreased as a result of microwave thawing treatment while increased in the other treatments.

scholarly journals Potential of Postharvest Coatings to Maintain Freshness of Red-Fleshed Pitaya (Hylocereus costaricensis)

Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 892
Author(s):  
Nur Azlin Razali ◽  
Steven A. Sargent ◽  
Charles A. Sims ◽  
Jeffrey K. Brecht ◽  
Adrian D. Berry ◽  
...  
Keyword(s):  
Weight Loss ◽  
Tap Water ◽  
Titratable Acidity ◽  
Quality Parameters ◽  
The Other ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Quality Factors ◽  
Climacteric Fruit

Pitaya is a non-climacteric fruit that has white or red flesh with numerous small, black seeds. It has a high moisture content; however, water loss during handling and storage negatively affects the fresh weight, firmness and appearance of the fruit, decreasing market value. Application of compatible postharvest coatings has been shown to benefit postharvest quality of many crops. The objective of this study was to evaluate the effect of two commercial coatings on weight loss and quality of pitaya during storage. Pitaya fruit were commercially harvested and sorted for uniformity of size and freedom from defects. Fruit were briefly immersed in either a vegetable oil-based coating (VOC; Sta-Fresh® 2981) or a carnauba-based coating (CC; Endura-Fresh™ 6100) according to manufacturer’s recommendations. Fruit immersed in tap water served as a control. Fruit were fan-dried at room temperature for 20 min, then stored at 7 °C with 85% relative humidity (RH) and evaluated for selected physical quality parameters each 5 days during 20 days. After each evaluation, fruit were peeled and frozen for later analysis of soluble solids content (SSC), total titratable acidity (TTA); on day 15 fresh samples were evaluated by an untrained consumer sensory panel. CC prevented exocarp shriveling for 15 days of storage, compared to uncoated pitaya (16.3% area affected); shriveling in VOC was intermediate and not significantly different from the other treatments. Mesocarp firmness remained constant throughout 15 days of storage regardless of treatment. Fruit exocarp h* angle increased slightly by day 20, becoming slightly less red, and there were no negative treatment effects for the other quality factors measured: SSC (11.33%), TTA (0.25%), weight loss (5.5%) or sensory evaluations (appearance, flavor, texture, firmness, and juiciness). After 20 days storage, appearance for fruit from all treatments was rated unacceptable due to development of anthracnose lesions. It was concluded that both CC and VOC maintained quality of pitaya for 15 days at 7 °C and 85% RH by delaying exocarp shriveling.

Catalytic Chemosensing Assay for Selective Detection of Methyl Parathion Organophosphate Pesticide

2019 ◽  
Vol 25 (41) ◽  
pp. 9643-9649 ◽  
Author(s):  
Anxun Zheng ◽  
Chang Shen ◽  
Qian Tang ◽  
Cheng‐Bin Gong ◽  
Cheuk‐Fai Chow
Keyword(s):  
Methyl Parathion ◽  
Selective Detection ◽  
Organophosphate Pesticide

scholarly journals TOXICIDADE AGUDA DO INSETICIDA PARATION METÍLICO E DO BIOPESTICIDA AZADIRACTINA DE FOLHAS DE NEEM (Azadirachta indica) PARA ALEVINO E JUVENIL DE PACU (Piaractus mesopotamicus)

2004 ◽  
Vol 14 ◽  
Author(s):  
CLAUDINEI CRUZ ◽  
JOAQUIM G. MACHADO-NETO ◽  
MANOEL LIMA DE MENEZES
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Environmental Risk ◽  
Azadirachta Indica ◽  
Methyl Parathion ◽  
Juvenile Fish ◽  
Aquatic Environments ◽  
Piaractus Mesopotamicus ◽  
Neem Leaves

Os objetivos deste trabalho foram calcular a CL (I) 50-96h do inseticida organofosforado paration metílico e do biopesticida azadiractina para alevinos e juvenis de pacu ( P. mesopotamicus). Também pretendeu-se avaliar o efeito do peso corpóreo sobre a toxicidade aguda do paration metílico e do neem para o pacu, bem como classificar o risco ambiental do uso de paration metílico e do neem para o controle de parasitas e patógenos de pacu. Foram realizados dois experimentos, em condições laboratoriais, para a determinação da concentração letal (CL (I) 50-96h). A CL (I) 50-96h calculada do paration metílico foi de 3,97 mg/L para os alevinos e de 9,89 mg/L para os juvenis. Para a azadiractina foi de 1,20 mg/L para os alevinos e de 1,18 mg/L para os juvenis. As concentrações de 1,0 mg/L de paration metílico para os alevinos e de 7,5 mg/L para os juvenis e as de 0,29 e 0,59 mg/L de azadiractina não provocaram mortalidade nos animais expostos e podem ser utilizadas como referência em estudos de controle de parasitas em pacu . O paration metílico foi menos tóxico para os alevinos e para os juvenis de pacu do que a azadiractina, indicando a necessidade de cuidados com a utilização de extratos aquosos de neem no ambiente aquático. ACUTE TOXICITY OF THE INSECTICIDE METHYL PARATHION AND OF THE BIOPESTICIDE AZADIRACHTIN FROM NEEM LEAVES (Azadirachta indica) TO ALEVINE AND JUVENILE PACU (Piaractus mesopotamicus) Abstract The objectives of the present study were: to calculate the lethal concentration LC (I) 50-96 h of methyl parathion and of the biopesticide azadirachtin to alevine and juvenile pacu ( P. mesopotamicus). Also to determine the effect of body weight on acute toxicity of methyl parathion and of neem for pacu, as well as to classify the environmental risk of the methyl parathion and neem uses for the control of pacu parasites and pathogens. Two experiments were performed under laboratory conditions for the determination of the LC (I) 50-96 h. The LC (I) 50-96 h of methyl parathion was 3.97 mg/L for alevine and 9.89 mg/L for juvenile fish. The LC (I) 50-96 h of azadirachtin was 1.20 mg/L for alevine and 1.18 mg/L for juvenile pacu. Concentrations of 1.0 and 7.5 mg/L of methyl parathion and of 0.29 and 0.59 mg/L of azadirachtin for alevine and juvenile pacu, respectively, did not cause mortality in the exposed animals and can be used as references in studies on the control of parasites in pacu. Methyl parathion was less toxic to alevine and juvenile pacu than azadirachtin, indicating the need for careful utilization of aqueous neem extracts in aquatic environments.

scholarly journals Brain Tissue Acidosis and Changes of Energy Metabolism in Mild Incomplete Ischemia—Topographical Study

1985 ◽  
Vol 5 (3) ◽  
pp. 432-438 ◽  
Author(s):  
Soo-Ho Kim ◽  
Hajime Handa ◽  
Masatsune Ishikawa ◽  
Osamu Hirai ◽  
Shinzo Yoshida ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Brain Tissue ◽  
The Other ◽  
Neurological Deficits ◽  
Anaerobic Glycolysis ◽  
Mongolian Gerbils ◽  
Tissue Ph ◽  
Tissue Acidosis ◽  
Energy Failure ◽  
Incomplete Ischemia

Regional changes of brain tissue pH and its correlation to energy metabolism were studied in various degrees of incomplete ischemia for 5 and 60 min in the unilateral common carotid occlusion of normally fed mongolian gerbils. The degree of ischemia was evaluated by the severity of neurological deficits following 60 min of occlusion, and animals were divided into three groups: symptomatic, borderline, and asymptomatic. Changes of NADH and ATP distribution corresponded well to the degree of ischemia. On the other hand, acidosis developed more clearly and extended in wider areas than the changes of NADH and ATP distribution. These changes were already seen at 5 min of occlusion. From the results of this experiment, it was suspected that acidosis in mild incomplete ischemia was due to stimulated anaerobic glycolysis that might supplement NADH oxidation and ATP yields. Further, acidosis without energy failure was considered not to be detrimental to neuronal cells.

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