Quantitative Determination of Reducing Sugars after Separation by Paper Chromatography

1959 ◽  
Vol 31 (10) ◽  
pp. 1615-1616 ◽  
Author(s):  
A. J. Philippu
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Reducing Sugars

ESTIMATION OF REDUCING SUGARS IN STARCH HYDROLYZATES BY PAPER CHROMATOGRAPHY

1950 ◽  
Vol 28b (9) ◽  
pp. 527-534 ◽  
Author(s):  
Ping Shu
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Chromatographic Separation ◽  
Reducing Sugars ◽  
Reducing Power ◽  
Probable Error

A method for quantitative determination of glucose, maltose, and reducing dextrins in starch hydrolyzates is described. The components are separated by paper chromatography and determined colorimetrically by measurement of their reducing power with alkaline copper and arseno-molybdate reagents. The probable error of the determinations with the use of this method is about 2%. A compact apparatus was designed, suitable for handling a large number of samples in the chromatographic separation. It is particularly useful for compounds with low Rf values.

Determination of Glucose in Egg Solids by Paper Chromatography

1964 ◽  
Vol 47 (3) ◽  
pp. 594-596
Author(s):  
Charles H Coleman ◽  
John E Despaul ◽  
Santiago Ruiz
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Chromatographic Method ◽  
Reducing Sugars ◽  
Residual Glucose ◽  
Reduction Methods ◽  
Egg Albumen

Abstract Copper reduction methods for determining residual glucose in egg solids give high apparent values due to the presence of other reducing sugars. A paper chromatographic method specific for glucose in dried egg and egg albumen has been developed in which glucose migrates independently of other components. Micro-quantities of glucose in egg from which glucose has been removed can be readily detected and determined. Migration of the unknown and its comparison with a standard glucose spot on the finished chromatogram provides positive qualitative identification and accurate quantitative determination. Good recoveries were obtained in micro-concentration ranges.

Quantitative Untersuchungen über die Ommochromvorstufen in den Malpighischen Gefäßen verschiedener Drosophila-Mutanten

1968 ◽  
Vol 23 (4) ◽  
pp. 547-554 ◽  
Author(s):  
Dieter Eichelberg
Keyword(s):  
Sex Differences ◽  
Drosophila Melanogaster ◽  
Quantitative Determination ◽  
Paper Chromatography ◽  
Type A ◽  
Individual Development ◽  
Wild Type ◽  
Strong Reduction ◽  
The Individual

This paper concerns with the quantitative determination of ommochrome precursors in the Malpighian tubes of Drosophila melanogaster during the individual development. After separation by paper chromatography the amounts of tryptophane, kynurenine and 3-hydroxykynurenine have been estimated by a spectrophotometer. The concentrations of these three substances obtained from wild-type Malpighian tubes have been compared with the quantities of the mutants brown (bw) and red Malpighian tubes (red). During development there are significant variabilities in contents of tryptophane, kynurenine and 3-hydroxykynurenine in the Malpighian tubes. In the larval tubes large quantities of ommochrome precursors are accumulated. With the beginning of metamorphosis there is a distinct decrease in these substances. After hatching the amount increases steadily until reaching a constant level. In the Malpighian tubes there are also sex differences: in females the concentration of kynurenine and 3-hydroxykynurenine is higher than in males. The results obtained from the mutants brown and red Malpighian tubes are on principle the same as those obtained from wild-type. A strong reduction of kynurenine contents is found in the mutant red Malpighian tubes. Perhaps in this mutant the kynurenine-hydroxilase-activity is lower than in wild-type. The amounts of ommochrome precursors, accumulated in the larval Malpighian tubes, do not correspond in all cases to the contents of xanthommatine formed in the eyes of the adults.

Quantitative determination of serum triglycerides by glass-fiber paper chromatography

1964 ◽  
Vol 8 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Karoly G. Pinter ◽  
James G. Hamilton ◽  
O. Neal Miller
Keyword(s):  
Quantitative Determination ◽  
Glass Fiber ◽  
Paper Chromatography ◽  
Serum Triglycerides
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