Quantitative determination of riboxine (9-?-d-ribofuranosylhypoxanthine) by the method of paper chromatography

1980 ◽  
Vol 14 (6) ◽  
pp. 420-423
Author(s):  
T. M. Kroshilova ◽  
V. G. Krunchak ◽  
V. S. Nyn'
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography

Quantitative Untersuchungen über die Ommochromvorstufen in den Malpighischen Gefäßen verschiedener Drosophila-Mutanten

1968 ◽  
Vol 23 (4) ◽  
pp. 547-554 ◽  
Author(s):  
Dieter Eichelberg
Keyword(s):  
Sex Differences ◽  
Drosophila Melanogaster ◽  
Quantitative Determination ◽  
Paper Chromatography ◽  
Type A ◽  
Individual Development ◽  
Wild Type ◽  
Strong Reduction ◽  
The Individual

This paper concerns with the quantitative determination of ommochrome precursors in the Malpighian tubes of Drosophila melanogaster during the individual development. After separation by paper chromatography the amounts of tryptophane, kynurenine and 3-hydroxykynurenine have been estimated by a spectrophotometer. The concentrations of these three substances obtained from wild-type Malpighian tubes have been compared with the quantities of the mutants brown (bw) and red Malpighian tubes (red). During development there are significant variabilities in contents of tryptophane, kynurenine and 3-hydroxykynurenine in the Malpighian tubes. In the larval tubes large quantities of ommochrome precursors are accumulated. With the beginning of metamorphosis there is a distinct decrease in these substances. After hatching the amount increases steadily until reaching a constant level. In the Malpighian tubes there are also sex differences: in females the concentration of kynurenine and 3-hydroxykynurenine is higher than in males. The results obtained from the mutants brown and red Malpighian tubes are on principle the same as those obtained from wild-type. A strong reduction of kynurenine contents is found in the mutant red Malpighian tubes. Perhaps in this mutant the kynurenine-hydroxilase-activity is lower than in wild-type. The amounts of ommochrome precursors, accumulated in the larval Malpighian tubes, do not correspond in all cases to the contents of xanthommatine formed in the eyes of the adults.

Quantitative determination of serum triglycerides by glass-fiber paper chromatography

1964 ◽  
Vol 8 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Karoly G. Pinter ◽  
James G. Hamilton ◽  
O. Neal Miller
Keyword(s):  
Quantitative Determination ◽  
Glass Fiber ◽  
Paper Chromatography ◽  
Serum Triglycerides

A solvent for qualitative and quantitative determination of sugars using paper chromatography

1960 ◽  
Vol 3 ◽  
pp. 343-350 ◽  
Author(s):  
P. Colombo ◽  
D. Corbetta ◽  
A. Pirotta ◽  
G. Ruffini ◽  
A. Sartori
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Qualitative And Quantitative

The Quantitative Determination of Mixed Sulfonamides by Paper Chromatography

1963 ◽  
Vol 46 (5) ◽  
pp. 899-901
Author(s):  
Frieda M Kunze ◽  
Luis Espinoza B
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Paper Chromatography ◽  
Short Wavelength ◽  
Mean Deviation ◽  
Marshall Technique ◽  
Quantitative Procedure

Abstract A simplified quantitative procedure for the determination of mixed sulfonamides in pharmaceuticals is presented. The components are resolved by paper chromatography and located by their fluorescence under short wavelength ultraviolet light. They are then eluted and determined colorimetrically by a revised Bratton-Marshall technique. Total time of analysis is less than 4 hours. Recoveries averaged 98.8% with a range of 95.7 to 103.4%. Mean deviation from the average was 1.4%.

A DOUBLE ISOTOPIC DERIVATIVE METHOD FOR THE QUANTITATIVE DETERMINATION OF OESTRONE AND 17β-OESTRADIOL IN PLASMA

1960 ◽  
Vol XXXV (II) ◽  
pp. 161-187 ◽  
Author(s):  
Reiner Svendsen
Keyword(s):  
Quantitative Determination ◽  
Pregnant Women ◽  
Paper Chromatography ◽  
Secondary Amenorrhea ◽  
Derivative Method ◽  
Menstruating Women

ABSTRACT A method with a sensitivity of about 2 ng is evolved for the determination of oestrone and 17β-oestradiol in plasma. The method is based on the double isotopic derivative principle and makes use of the isotopes 35S and 131I. The method includes: Extraction of plasma, purification of the extract, esterification of oestrone and 17β-oestradiol by means of p-iodobenzenesulphonyl chloride, purification of the esters formed by paper chromatography, counting of the radioactivities and calculation of the results. The procedures are described in detail and discussed. Determinations in plasma gave the following ranges of concentrations: Normally menstruating women, oestrone and 17β-oestradiol: 0.1–0.75 μg/l, some patients with primary or secondary amenorrhea, oestrone and 17β-oestradiol: 0–0.45 μg/l and pregnant women, oestrone: 1–10 μg/l and 17β-oestradiol: 2–30 μg/l.

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