Simple Setup for Gas-Phase H/D Exchange Mass Spectrometry Coupled to Electron Transfer Dissociation and Ion Mobility for Analysis of Polypeptide Structure on a Liquid Chromatographic Time Scale

2014 ◽  
Vol 86 (23) ◽  
pp. 11868-11876 ◽  
Author(s):  
Ulrik H. Mistarz ◽  
Jeffery M. Brown ◽  
Kim F. Haselmann ◽  
Kasper D. Rand
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Gas Phase ◽  
Ion Mobility ◽  
Time Scale ◽  
Electron Transfer Dissociation ◽  
Exchange Mass Spectrometry ◽  
Polypeptide Structure ◽  
Liquid Chromatographic

scholarly journals Analysis of phosphoinositide 3-kinase inhibitors by bottom-up electron-transfer dissociation hydrogen/deuterium exchange mass spectrometry

2017 ◽  
Vol 474 (11) ◽  
pp. 1867-1877 ◽  
Author(s):  
Glenn R. Masson ◽  
Sarah L. Maslen ◽  
Roger L. Williams
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Electron Transfer Dissociation ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Phosphoinositide 3 Kinase ◽  
Hdx Ms

Until recently, one of the major limitations of hydrogen/deuterium exchange mass spectrometry (HDX-MS) was the peptide-level resolution afforded by proteolytic digestion. This limitation can be selectively overcome through the use of electron-transfer dissociation to fragment peptides in a manner that allows the retention of the deuterium signal to produce hydrogen/deuterium exchange tandem mass spectrometry (HDX-MS/MS). Here, we describe the application of HDX-MS/MS to structurally screen inhibitors of the oncogene phosphoinositide 3-kinase catalytic p110α subunit. HDX-MS/MS analysis is able to discern a conserved mechanism of inhibition common to a range of inhibitors. Owing to the relatively minor amounts of protein required, this technique may be utilised in pharmaceutical development for screening potential therapeutics.

scholarly journals A broader view on ion heating in traveling-wave devices using fragmentation of CsI clusters and extent of H˙ migration as molecular thermometers

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3388-3399 ◽  
Author(s):  
Frederik Lermyte ◽  
Frank Sobott
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Ion Mobility ◽  
Traveling Wave ◽  
Electron Transfer Dissociation ◽  
Ion Heating ◽  
Molecular Thermometers

Traveling-wave-dependent ion ‘heating’ is observed during mass spectrometry experiments under both ion mobility and electron transfer dissociation conditions and investigated using novel molecular ‘thermometers’.

scholarly journals Gas-Phase Rearrangement in Lysine Phosphorylated Peptides During Electron-Transfer Dissociation Tandem Mass Spectrometry

2015 ◽  
Vol 87 (14) ◽  
pp. 6990-6994 ◽  
Author(s):  
Jordi Bertran-Vicente ◽  
Michael Schümann ◽  
Christian P. R. Hackenberger ◽  
Eberhard Krause
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Tandem Mass Spectrometry ◽  
Gas Phase ◽  
Electron Transfer Dissociation ◽  
Tandem Mass ◽  
Phosphorylated Peptides

scholarly journals Application of electron transfer dissociation mass spectrometry in analyses of non-enzymatically glycated peptides

2007 ◽  
Vol 21 (5) ◽  
pp. 661-666 ◽  
Author(s):  
Qibin Zhang ◽  
Andrej Frolov ◽  
Ning Tang ◽  
Ralf Hoffmann ◽  
Tom van de Goor ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Electron Transfer Dissociation
Sign in / Sign up

Export Citation Format

Share Document