A Label-Free Bioluminescent Sensor for Real-Time Monitoring Polynucleotide Kinase Activity

2014 ◽  
Vol 86 (16) ◽  
pp. 8481-8488 ◽  
Author(s):  
Jiao Du ◽  
Qinfeng Xu ◽  
Xiaoquan Lu ◽  
Chun-yang Zhang
Keyword(s):  
Real Time ◽  
Kinase Activity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Polynucleotide Kinase

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy

2013 ◽  
Vol 176 ◽  
pp. 1176-1182 ◽  
Author(s):  
Yuki Aonuma ◽  
Yasuhiko Kondo ◽  
Ayumi Hirano-Iwata ◽  
Atena Nishikawa ◽  
Yasuo Shinohara ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Adipocyte Differentiation ◽  
Label Free ◽  
Real Time Monitoring

Label-free optical biosensor for real-time monitoring the cytotoxicity of xenobiotics: A proof of principle study on glyphosate

2018 ◽  
Vol 351 ◽  
pp. 80-89 ◽  
Author(s):  
Eniko Farkas ◽  
Andras Szekacs ◽  
Boglarka Kovacs ◽  
Marianna Olah ◽  
Robert Horvath ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Biosensor ◽  
Label Free ◽  
Real Time Monitoring ◽  
Proof Of Principle

scholarly journals Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 478 ◽  
Author(s):  
Laura Pol ◽  
Chris Eckstein ◽  
Laura Acosta ◽  
Elisabet Xifré-Pérez ◽  
Josep Ferré-Borrull ◽  
...  
Keyword(s):  
Surface Modification ◽  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Real Time Monitoring ◽  
Helpful Tool ◽  
Nanoporous Anodic Alumina ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Molecule Model

The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.

Real time monitoring and label free creatinine detection with artificial receptors

2019 ◽  
Vol 244 ◽  
pp. 6-11 ◽  
Author(s):  
Aykut Arif Topçu ◽  
Erdoğan Özgür ◽  
Fatma Yılmaz ◽  
Nilay Bereli ◽  
Adil Denizli
Keyword(s):  
Real Time ◽  
Label Free ◽  
Real Time Monitoring ◽  
Artificial Receptors

Noninvasive label-free nanoplasmonic optical imaging for real-time monitoring of in vitro amyloid fibrogenesis

Nanoscale ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 3561-3565 ◽  
Author(s):  
Sung Sik Lee ◽  
Luke P. Lee
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Optical Imaging ◽  
Real Time ◽  
Label Free ◽  
Real Time Monitoring ◽  
Pathological Mechanism

We utilize nanoplasmonic optical imaging as the noninvasive and label-free method in order to monitorin vitroamyloid fibrogenesis in real-time, which is considered as the primary pathological mechanism of Alzheimer's disease.

Impedance spectroscopy based measurement system for quantitative and label-free real-time monitoring of tauopathy in hippocampal slice cultures

2012 ◽  
Vol 32 (1) ◽  
pp. 250-258 ◽  
Author(s):  
Heinz-Georg Jahnke ◽  
Annett Braesigk ◽  
Till G.A. Mack ◽  
Sarah Pönick ◽  
Frank Striggow ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Real Time ◽  
Measurement System ◽  
Hippocampal Slice ◽  
Label Free ◽  
Real Time Monitoring ◽  
Hippocampal Slice Cultures

Label-Free, Real-Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy

2010 ◽  
Vol 49 (32) ◽  
pp. 5476-5479 ◽  
Author(s):  
Brian G. Saar ◽  
Yining Zeng ◽  
Christian W. Freudiger ◽  
Yu-San Liu ◽  
Michael E. Himmel ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Real Time ◽  
Stimulated Raman Scattering ◽  
Label Free ◽  
Real Time Monitoring ◽  
Biomass Processing ◽  
Stimulated Raman
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