Unravelling Nonspecific Adsorption of Complex Protein Mixture on Surfaces with SPR and MS

2014 ◽  
Vol 86 (19) ◽  
pp. 9612-9619 ◽  
Author(s):  
Julien Breault-Turcot ◽  
Pierre Chaurand ◽  
Jean-Francois Masson
Keyword(s):  
Nonspecific Adsorption ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Fractionation of Complex Protein Mixture by Virtual Three-Dimensional Liquid Chromatography Based on Combined pH and Salt Steps

2008 ◽  
Vol 7 (10) ◽  
pp. 4525-4537 ◽  
Author(s):  
Zhi-Bin Ning ◽  
Qing-Run Li ◽  
Jie Dai ◽  
Rong-Xia Li ◽  
Chia-Hui Shieh ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

The role of proteomics in studies of protein moonlighting

2014 ◽  
Vol 42 (6) ◽  
pp. 1698-1703 ◽  
Author(s):  
Robert J. Beynon ◽  
Dean Hammond ◽  
Victoria Harman ◽  
Yvonne Woolerton
Keyword(s):  
Post Translational Modifications ◽  
Multiple Functions ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Quantitative Approaches

The increasing acceptance that proteins may exert multiple functions in the cell brings with it new analytical challenges that will have an impact on the field of proteomics. Many proteomics workflows begin by destroying information about the interactions between different proteins, and the reduction of a complex protein mixture to constituent peptides also scrambles information about the combinatorial potential of post-translational modifications. To bring the focus of proteomics on to the domain of protein moonlighting will require novel analytical and quantitative approaches.

Matrigel: A complex protein mixture required for optimal growth of cell culture

PROTEOMICS ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 1886-1890 ◽  
Author(s):  
Chris S. Hughes ◽  
Lynne M. Postovit ◽  
Gilles A. Lajoie
Keyword(s):  
Cell Culture ◽  
Optimal Growth ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Comparison of displacement versus gradient mode for separation of a complex protein mixture by anion-exchange chromatography

2012 ◽  
Vol 901 ◽  
pp. 34-40 ◽  
Author(s):  
Robert Ahrends ◽  
Björn Lichtner ◽  
Friedrich Buck ◽  
Diana Hildebrand ◽  
Marta Kotasinska ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Gradient Mode

Clickable Cellulosic Surfaces for Peptide-Based Bioassays

2019 ◽  
Author(s):  
Maria Teresa Odinolfi ◽  
Alessandro Romanato ◽  
Greta Bergamaschi ◽  
Alessandro Strada ◽  
Laura Sola ◽  
...  
Keyword(s):  
Target Recognition ◽  
Real Case ◽  
Polymeric Coating ◽  
Healthy Controls ◽  
Nonspecific Adsorption ◽  
Cellulosic Materials ◽  
Cellulose Substrates

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.

Clickable Cellulosic Surfaces for Peptide-Based Bioassays

2019 ◽  
Author(s):  
Maria Teresa Odinolfi ◽  
Alessandro Romanato ◽  
Greta Bergamaschi ◽  
Alessandro Strada ◽  
Laura Sola ◽  
...  
Keyword(s):  
Target Recognition ◽  
Real Case ◽  
Polymeric Coating ◽  
Healthy Controls ◽  
Nonspecific Adsorption ◽  
Cellulosic Materials ◽  
Cellulose Substrates

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.

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