Matrigel: A complex protein mixture required for optimal growth of cell culture

PROTEOMICS ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 1886-1890 ◽  
Author(s):  
Chris S. Hughes ◽  
Lynne M. Postovit ◽  
Gilles A. Lajoie
Keyword(s):  
Cell Culture ◽  
Optimal Growth ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Fractionation of Complex Protein Mixture by Virtual Three-Dimensional Liquid Chromatography Based on Combined pH and Salt Steps

2008 ◽  
Vol 7 (10) ◽  
pp. 4525-4537 ◽  
Author(s):  
Zhi-Bin Ning ◽  
Qing-Run Li ◽  
Jie Dai ◽  
Rong-Xia Li ◽  
Chia-Hui Shieh ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

The role of proteomics in studies of protein moonlighting

2014 ◽  
Vol 42 (6) ◽  
pp. 1698-1703 ◽  
Author(s):  
Robert J. Beynon ◽  
Dean Hammond ◽  
Victoria Harman ◽  
Yvonne Woolerton
Keyword(s):  
Post Translational Modifications ◽  
Multiple Functions ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Quantitative Approaches

The increasing acceptance that proteins may exert multiple functions in the cell brings with it new analytical challenges that will have an impact on the field of proteomics. Many proteomics workflows begin by destroying information about the interactions between different proteins, and the reduction of a complex protein mixture to constituent peptides also scrambles information about the combinatorial potential of post-translational modifications. To bring the focus of proteomics on to the domain of protein moonlighting will require novel analytical and quantitative approaches.

Unravelling Nonspecific Adsorption of Complex Protein Mixture on Surfaces with SPR and MS

2014 ◽  
Vol 86 (19) ◽  
pp. 9612-9619 ◽  
Author(s):  
Julien Breault-Turcot ◽  
Pierre Chaurand ◽  
Jean-Francois Masson
Keyword(s):  
Nonspecific Adsorption ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Comparison of displacement versus gradient mode for separation of a complex protein mixture by anion-exchange chromatography

2012 ◽  
Vol 901 ◽  
pp. 34-40 ◽  
Author(s):  
Robert Ahrends ◽  
Björn Lichtner ◽  
Friedrich Buck ◽  
Diana Hildebrand ◽  
Marta Kotasinska ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Gradient Mode

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

Sign in / Sign up

Export Citation Format

Share Document