New Approach for Development of Sensitive and Environmentally Friendly Immunoassay for Mycotoxin Fumonisin B1Based on Using Peptide-MBP Fusion Protein as Substitute for Coating Antigen

2014 ◽  
Vol 86 (16) ◽  
pp. 8433-8440 ◽  
Author(s):  
Yang Xu ◽  
Bo Chen ◽  
Qing-hua He ◽  
Yu-Lou Qiu ◽  
Xing Liu ◽  
...  
2018 ◽  
Vol 54 (59) ◽  
pp. 8265-8268 ◽  
Author(s):  
Hong-hua Zhang ◽  
Yong-qiang Wang ◽  
Long-tao Huang ◽  
Long-qing Zhu ◽  
Yi-yue Feng ◽  
...  

A new approach for the synthesis of isatins and isoindigoes by an inexpensive and environmentally friendly NaI-mediated transformation is disclosed.


BioResources ◽  
2015 ◽  
Vol 10 (4) ◽  
Author(s):  
María Cecilia Basso ◽  
Antonio Pizzi ◽  
Luc Delmotte

Coatings ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 492 ◽  
Author(s):  
Andreas Breitwieser ◽  
Philipp Siedlaczek ◽  
Helga Lichtenegger ◽  
Uwe B. Sleytr ◽  
Dietmar Pum

Carbon nanotubes (CNTs) have already been considered for medical applications due to their small diameter and ability to penetrate cells and tissues. However, since CNTs are chemically inert and non-dispersible in water, they have to be chemically functionalized or coated with biomolecules to carry payloads or interact with the environment. Proteins, although often only randomly bound to the CNT surface, are preferred because they provide a better biocompatibility and present functional groups for binding additional molecules. A new approach to functionalize CNTs with a closed and precisely ordered protein layer is offered by bacterial surface layer (S-layer) proteins, which have already attracted much attention in the functionalization of surfaces. We could demonstrate that bacterial S-layer proteins (SbpA of Lysinibacillus sphaericus CCM 2177 and the recombinant fusion protein rSbpA31-1068GG comprising the S-layer protein and two copies of the IgG binding region of Protein G) can be used to disperse and functionalize oxidized multi walled CNTs. Following a simple protocol, a complete surface coverage with a long-range crystalline S-layer lattice can be obtained. When rSbpA31-1068GG was used for coating, the introduced functionality could be confirmed by binding gold labeled antibodies via the IgG binding domain of the fusion protein. Since a great variety of functional S-layer fusion proteins has already been described, our new technology has the potential for a broad spectrum of functionalized CNTs.


2014 ◽  
Vol 62 (35) ◽  
pp. 8830-8836 ◽  
Author(s):  
Yang Xu ◽  
Zhenyun He ◽  
Qinghua He ◽  
Yulou Qiu ◽  
Bo Chen ◽  
...  

Energies ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 2492 ◽  
Author(s):  
Maurizio Albano ◽  
A. Haddad ◽  
Huw Griffiths ◽  
Paul Coventry

This paper investigates the possible options for achieving a substantial reduction in a substation footprint using air-insulated switchgear as a more environmentally-friendly alternative to gas-insulated substations that use SF6 gas. Adopting a new approach to surge arrester location and numbers, International Electrotechnical Commission (IEC) minimum clearances can be successfully selected instead of the maximum clearances as currently adopted by many utilities, as is the case in the UK. In addition, innovative alternative compact busbar arrangements using vertical and delta configurations have been proposed by the authors. A further opportunity for compaction is offered by the application of compact and integrated technology offered from several manufacturers. The full overvoltage control within the entire substation under any surge condition is a key aspect of the feasibility of this type of substation. This work demonstrates that the new design option can be an attractive alternative for future substation configuration with minimum footprint.


SAGE Open ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 215824402110066
Author(s):  
Rolandas Drejeris ◽  
Astrida Miceikiene ◽  
Jurgita Baranauskiene

This article suggests a new concept of entrepreneurship based on a modern approach about competition between agribusiness entities and according to the area of their commercial activity. The importance of entrepreneurship measurement is grounded and the correctness of the methodology is proven. Creating a model that covers all aspects of entrepreneurship measurement was determined as the aim of this article. This article presents a real, innovative methodology for measuring entrepreneurship in the agricultural sector based on the modification of TOPSIS method. The method chosen was recognized as the most appropriate for determining the level of entrepreneurship, which is expressed by quantitative parameters. Using the modification of multicriteria TOPSIS method for measuring entrepreneurship in agricultural entities, 12 specific criteria were selected. The model has been tested in the livestock farms of Lithuania to include entrepreneurship as a criterion in assessing the potential of these farms and their readiness to apply sustainable and environmentally friendly activities to organic farming. The applicability of the model has been directly confirmed. The use of the model makes it possible to rank agricultural businesses in a given region according to their degree of entrepreneurship.


2021 ◽  
Vol 4 (2) ◽  
pp. 269-275
Author(s):  
Belinda Sukapura Dewi ◽  
ARLETI M. APIN ◽  
Ariesa Pandanwangi ◽  
Nuning Damayanti

Batik is known not only for its beauty, but also for the values ​​it contains. This potential is very inspiring and gives an idea visualized into batik storytelling using the cold method of tamarin paste, a new approach that is more environmentally friendly. This technique has also been tested both domestically and abroad and has great potential for opportunities batik development with new ideas. An opportunity to preserve batik novelty that have no potential harass to traditional batik. This study applies the discussion of visual language to folklore theme batik works. In the earlier times, speech was common culture carried by elders to their children or grandchildren. Folktales in the form of legends, myths and fables are moral messages way conveyed in a friendly, fun but also educating with devotion. The theories and methods used to discuss two batik samples are taken from folklore which contains educational moral values. Based on a study of selected samples of batik works, it is known that the images or shapes on batik use the Space Time Flat (RWD) and Naturalist Perspective Moment Opname (NPM) systems simultaneously. Most of the depictions of motifs and shapes in batik still use the RWD system, although some forms also use NPM.


2020 ◽  
Vol 295 (10) ◽  
pp. 2913-2914 ◽  
Author(s):  
Philip M. M. Ruppert ◽  
Sander Kersten

The enzyme lipoprotein lipase (LPL) is responsible for breaking down triglycerides in the blood. Mutations in LPL cause a rare but debilitating disorder characterized by excessive plasma triglyceride levels for which treatment options are limited. Nimonkar et al. now present a fusion protein between LPL and its physiological transporter GBIHBP1 that is highly active and largely resistant to physiological inhibitors of LPL. Injecting this fusion protein effectively lowers plasma triglycerides in mice and represents a promising new approach for lowering triglycerides in patients with familial chylomicronemia syndrome.


2014 ◽  
Vol 25 (0) ◽  
pp. 59
Author(s):  
Kaloyan D. Georgiev ◽  
Dobrin D. Georgiev ◽  
Dobrin S. Georgiev
Keyword(s):  

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